|本期目录/Table of Contents|

[1]安蓬蓬,汤 华,郭军飞.?血浆miRNA-372/373在正常献血者和HBV早期感染献血者中的表达差异[J].天津医科大学学报,2015,21(06):503-506.
　AN Peng-peng,TANG Hua,GUO Jun-fei.Differential expression of miRNA-372/373 in plasma between the normal donors and the HBV donors at early stage[J].Journal of Tianjin Medical University,2015,21(06):503-506.
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?血浆miRNA-372/373在正常献血者和HBV早期感染献血者中的表达差异(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
21卷
期数:
2015年06期
页码:
503-506
栏目:
临床医学
出版日期:
2015-11-20

文章信息/Info

Title:
Differential expression of miRNA-372/373 in plasma between the normal donors and the HBV donors at early stage
文章编号:
1006-8147（2015）06-0503-04
作者:
安蓬蓬1 ; 汤 华2 ; 郭军飞2
（1.天津市血液中心质管科，天津300110 ；2.天津医科大学天津市生命科学中心实验室 天津300070）
Author(s):
?AN Peng-peng1; TANG Hua2;  GUO Jun-fei2

（1. Department of Quality Control ,Tianjin Blood Center, Tianjin 300110，China ；2.Tianjin Life Science Research Center, Tianjin Medical University, Tianjin 300070，China）

关键词:
微小RNA; miRNA-372; miRNA-373;  HBV早期感染
Keywords:
microRNA ;  miRNA-372 ; miRNA-373 ; the early-stage infection of HBV
分类号:
R457.1
DOI:
-
文献标志码:
A
摘要:
目的： 研究miRNA-372/373与HBV早期感染的相关性，分析其在HBV早期感染诊断中的潜在应用价值。方法： 收集来源于2013年3月至9月天津市内无偿献血者所献血液的血浆标本留样，共54份，分为正常组、酶免单阳组(E单阳组)和酶免核酸双阳组（EN双阳组）。提取此3组共54份血浆标本中的miRNA-372和miRNA-373，用实时荧光定量PCR方法进行检测。最后，分别比较miRNA-372和miRNA-373在3组血浆标本之间的表达差异，并对各组间的miRNA表达趋势进行统计学分析；同时，对miRNA-372、miRNA-373的相对表达水平分别与核酸检测HBV核酸阳性血浆标本中的HBV DNA拷贝数进行相关性分析。结果： 经实时荧光定量PCR检测，与正常献血者相比，miRNA-372、miRNA-373在:E单阳组和EN双阳组中均表达上调，其中，miRNA-372、miRNA-373在EN双阳组中表达上调更为显著。进一步分析发现，在EN双阳组中，miRNA-372、miRNA-373均与相应的HBV DNA拷贝数呈正相关性，其中，与miRNA-372相比，miRNA-373与相应的HBV DNA拷贝数的正相关性更为明显。结论： miRNA-372、miRNA-373与HBV早期感染密切相关，提示miRNA-372、miRNA-373在HBV感染的早期诊断中具有潜在价值。
Abstract:

Objective: To explore the relationship between the miRNA-372/373 levels in plasma and the early-stage infection of HBV, to uncover the potential

of miRNA-372/373 in the diagnosis of the early-stage infection of HBV. Methods: Blood samples for miRNA detecting were collected from 54 donors who donated blood from 2013.3 to 2013.9 in Tianjin. All the samples were divided into 3 groups: eighteen samples were Normal; 18 samples were ELISA testing (+) and NAT (-) (HBV-S); the last 18 samples were ELISA testing (+) and NAT(+) (HBV-D). Quantization of miRNA-372 and miRNA-373 in all the 54 plasma samples were performed using quantitative RT-PCR. At last, compared thederegulated miRNA(miRNA-372/-373) in the plasma of the three groups respectively, and the expression patterns of miRNA-372 andmiRNA-373 were analyzed using statistical methods; the correlation between the expression level of miRNA-372/-373 and the copy number of HBV DNA in NAT(+) plasma was statistically analyzed. Results: Compared with the Normal, miRNA-372 and miRNA-373 in
?both HBV-S and HBV-D were up-regulated. Furthermore, both miRNA-372 and miRNA-373 were up-regulated more significantly in HBV-D than in HBV-S. Moreover, the levels of both miRNA-372 and miRNA-373 were positively correlated with the copy number of HBV DNA in HBV-D. Compared with miRNA-372, miRNA-373’s positive correlation with the copy number of HBV DNA was significantly higher. Conclusion: The correlation between miRNA-372/373 in plasma
and the early-stage Infection of HBV is high , indicating their potential values in the early-stage diagnosis of HBV infection.

参考文献/References:

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备注/Memo

备注/Memo:

作者简介：安蓬蓬（1979-），女，主治医师，硕士在读，研究方向：输血；

通信作者：汤华，E-mail：htang2002@yahoo.com。

更新日期/Last Update: 2015-11-27