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[1]唐培源,戚 斌,陈 渊,等.shRNA干扰胸腺瘤细胞Wnt4基因后JNK基因表达下调[J].天津医科大学学报,2015,21(03):212-216.
　TANG Pei-yuan,QI Bin,CHEN Yuan,et al. Expression of JNK gene reduced by using shRNA interference Wnt4 gene in thymoma cells [J].Journal of Tianjin Medical University,2015,21(03):212-216.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
21卷
期数:
2015年03期
页码:
212-216
栏目:
基础医学
出版日期:
2015-05-20

文章信息/Info

Title:

Expression of JNK gene reduced by using shRNA interference Wnt4 gene in thymoma cells

 

文章编号:
1006-8147（2015）03-0212-05
作者:

唐培源 ; 戚 斌 ; 陈 渊 ; 王元国 ; 张 鹏

（天津医科大学总医院心胸外科，天津 300052）
Author(s):
TANG Pei-yuan;  QI Bin;  CHEN Yuan;  WANG Yuan-guo;  ZHANG Peng
(Department of Cardiothoracic Surgery, General Hospital, Tianjin Medical University, Tianjin 300052, China)
关键词:
胸腺瘤; Wnt4; JNK; 干扰; shRNA
Keywords:
Thymoma; Wnt4; JNK; interference; shRNA
分类号:
R736.3
DOI:
-
文献标志码:
A
摘要:
目的：研究shRNA干扰Wnt4基因对胸腺瘤细胞JNK基因表达的影响及Wnt4基因发挥作用可能依赖的信号通路。方法: 根据 shRNA 设计原则，设计针对Wnt4 基因的4个干扰靶点，构建 Wnt4-shRNA干扰质粒，经鉴定片段成功插入后，将空白质粒、TR001质粒和重组的4个干扰质粒分别转染体外培养的胸腺瘤细胞。采用RT-PCR检测转染后Wnt4基因表达，挑选对 Wnt4基因抑制效果最好的干扰质粒进行后续实验。将胸腺瘤细胞分3组：空白对照组；转染TR001质粒组；转染干扰质粒组；用LipofectamineTM2000 转染，采用RT-PCR 及Western blot 检测细胞 Wnt4和JNK的 mRNA 及蛋白表达。结果：shRNA- Wnt4-3质粒能显著抑制Wnt4基因表达，抑制率为52.37%（P＜0.05）。Wnt4基因下调后胸腺瘤细胞内JNK基因表达也显著下调（P＜0.01），差异有统计学意义。结论：采用shRNA干扰Wnt4基因后胸腺瘤细胞内JNK基因表达显著下调，说明Wnt4基因与JNK基因的表达有相关性, Wnt4基因可能依赖于非经典的Wnt/JNK信号通路发挥作用。
Abstract:
Objective: To explore the effect of Wnt4 gene on the expression of JNK gene and the signal pathway in thymoma cells. Methods: Four Wnt4-shRNA interference plasmids were constructed to target directly on Wnt4 gene. The blank plasmid, TR001 and recombinant plasmids were transfected into thymoma cells. The expression of Wnt4 was detected by RT-PCR while the best Wnt4 interference plasmid for subsequent experiments was selected. The thymoma cells were divided into three groups: control group, transfected TR001 plasmid, transfected interference plasmid. The expression of Wnt4 and JNK were detected by RT-PCR and Western blot. Results: Expression of Wnt4 could be significantly inhibited by could significantly inhibit.The ratio of inhibition was 52.37%（P＜0.05）.With down regulation of Wnt4, the expression of JNK were significantly down-regulated in thymoma cells (P＜0.01). Conclusion: After interfering Wnt4 gene by using shRNA , the expression of JNK gene is significantly reduced. This indicates that Wnt4 gene is correlated with the expression of JNK gene in thymoma cells.Which may depend on the non-canonical Wnt / JNK signaling pathway.

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相似文献/References:

[1]孟繁杰 综述,王广舜 审校.胸腺瘤与自身免疫性疾病的研究进展[J].天津医科大学学报,2021,27(02):195.

备注/Memo

备注/Memo:
基金项目 高等学校博士学科点专项科研基金（20121202110009）
作者简介 唐培源（1988- ），男，硕士在读，胸部肿瘤:

通信作者：张鹏，E-mail：zhp_tjgh@163.com。

更新日期/Last Update: 2015-05-27