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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

19卷

期数:

2013年06期

页码:

449-451

栏目:

基础医学

出版日期:

2013-11-20

文章信息/Info

Title:

Protective effects of hydrogen gas on survival rate and liver injury in septic mice

作者:

高秋文; 谢克亮; 王卫娜; 陈红光; 王国林; 于泳浩

天津医科大学总医院麻醉科，天津市麻醉学研究所，天津 300052

Author(s):

GAO Qiu-wen;  XIE Ke-liang;  WANG Wei-na;  CHEN Hong-guang;  WANG Guo-lin;  YU Yong-hao

Department of anesthesiology, Tianjin Institute of Anesthesiology, General Hospital, Tianjin Medical University, Tianjin 300052, China

关键词:

氢气; 脓毒症; 肝损伤; 氧化应激; 小鼠

Keywords:

hydrogen gas;  sepsis;  liver injury;  oxidative stress;  mice

分类号:

R730.231+R735.7

DOI:

-

文献标志码:

A

摘要:

目的：探讨氢气对酵母多糖（ZY）诱导的脓毒症小鼠存活率和肝脏损伤的影响及相关机制。方法：雄性ICR野生型小鼠120只，体质量20～25 g，5周龄，采用随机数字表法分为4组（n=30）：正常对照组（A组）、氢气组（B组）、ZY组（C组）、ZY＋氢气组（D组）。C和D组小鼠腹腔注射ZY制备脓毒症模型；B和D组小鼠在腹腔注射后的1 h和6 h时分别吸入2％氢气1 h。每组取20只观察7 d内小鼠生存情况。另40只动物，实验后24 h下腔静脉采血，取肝脏组织，检测血清丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）水平及进行肝脏组织病理评分，测定血清和肝脏超氧化物歧化酶（SOD）及8-异前列腺素F2α（8-iso-PGF2α）水平。结果：与A组相比，C组动物存活率降低，血清AST、ALT水平及肝脏病理评分升高，血清和肝脏组织SOD活性降低，而8-iso-PGF2α水平明显升高（P<0.05）；与C组相比，D组存活率提高，血清AST和ALT水平及肝脏病理评分降低，血清和肝脏组织中SOD活性升高，而 8-iso-PGF2α水平降低（P<0.05）。结论：氢气可改善脓毒症小鼠存活率，减轻肝脏损伤，其机制可能与抑制氧化应激反应有关。

Abstract:

Objective: To investigate the protective effects of hydrogen gas (H2) on survival rate and liver injury in zymosan (ZY)-induced septic mice. Methods: One hundred and twenty male ICR mice, aged 5 weeks, weighing 20-25 g, were randomly divided into 4 groups (n =30 each): control group (group A), H2 group (group B), ZY group (group C) and ZY + H2 group (group D). Septic mice were produced by peritoneal injection of ZY in groups C and D. Mice in groups B and D received 1 h inhalation of 2％ H2 at 1 and 6 h after normal saline or ZY injection. Twenty animals in each group were selected and observed for the 7 d survival rates. The left 10 animals in each group were sacrificed at 24 h after injection. Venous blood samples and liver tissues were obtained to determine the levels of superoxide dismutase (SOD) and 8-iso-prostaglandin F2alpha (8-iso-PGF2α). Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were tested, meanwhile liver histopathology were examined. Results: Compared with group A, the animals in group C showed a significant decrease of 7 d survival rate, liver injury (the increase of serum ALT, AST and histopathological scores), the decrease of SOD activities and increase of 8-iso-PGF2α levels in serum and liver tissues (P<0.05). Compared with group C, the animals in group D showed a significant increase of 7 d survival rate, improvement of liver injury (the increase of serum ALT, AST and histopathological scores), increase of SOD activities and decrease of 8-iso-PGF2α levels in serum and liver tissues (P<0.05). Conclusion: Hydrogen can improve the survival rate and liver injury in septic mice via inhibiting oxidative stress.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金资助项目（81071533,81101409）；天津市应用基础及前沿技术研究计划资助（11JCYBJC12900,13JCQNJC11400）；天津市卫生局资助项目（2011KZ108） 作者简介：髙秋文（1968-），女，主管护师，研究方向：麻醉护理；通信作者：于泳浩，E-mail: yuyonghao@163.com。

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更新日期/Last Update: 2013-12-20