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[1]李萌乾,王青松,郭正隆,等.诱导型狨猴肝纤维化模型的建立 [J].天津医科大学学报,2014,20(06):433-436.
　LI Meng-qian,WANG Qing-song,GUO Zheng-long,et al. Establishment of drug-induced common marmoset model for hepatic fibrosis[J].Journal of Tianjin Medical University,2014,20(06):433-436.
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诱导型狨猴肝纤维化模型的建立


 
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
20卷
期数:
2014年06期
页码:
433-436
栏目:
基础医学
出版日期:
2014-11-20

文章信息/Info

Title:
 Establishment of drug-induced common marmoset model for hepatic fibrosis
文章编号:
1006-8147（2014）06-0433-04
作者:
李萌乾; 王青松; 郭正隆; 尹海芳
（天津医科大学基础医学研究中心，天津医科大学细胞生物学系，天津 300070）
Author(s):
LI Meng-qian; WANG Qing-song; GUO Zheng-long; YIN Hai-fang
(Research Centre of Basic Medical Science，Department of Cell Biology, Tianjin Medical University, Tianjin 300070, China)
关键词:
肝纤维化; 普通狨猴; 二乙基亚硝胺; 肝切除
Keywords:
hepatic fibrosis;  common marmoset;  diethylnirtosamine; hepatectomy
分类号:
R575.2
DOI:
-
文献标志码:
A
摘要:
目的：建立诱导型狨猴肝纤维化模型。方法：手术切除普通狨猴部分肝脏，通过腹腔注射二乙基亚硝胺（DENA-2.5 mg/kg)，并利用体质量和肝功能检测、病理分析、免疫组化，胶原染色和B超小动物活体成像等手段进行监测。结果：手术切除肝脏促使狨猴肝脏再生，并伴随肥大和炎症反应；DENA药物诱导会引发与人类类似的肝脏炎症，肝脏胶原聚集和血管增生，最终导致狨猴肝脏纤维化。结论：手术切肝联合DENA化学诱导可以成功获得狨猴肝纤维化模型。
Abstract:
Objective: To establish the drug-induced common marmoset model for hepatic fibrosis. Methods: Hepatectomy and intraperitoneal injection of DENA at the dose of 2.5 mg/kg was used and various assays were performed to monitor the bodyweight, liver function, pathology, and collagen in common marmosets. Furthermore, non-invasive imaging systems were applied i.e. B ultrasound and live animal imaging. Results: The generation of newborn liver and inflammation was accelerated by hepatectomy. DENA was effective in inducing hepatitis, vascular proliferation, accumulation of collagen and fibrosis as observed in clinic. Conclusion: Successfully establishes the common marmoset model for hepatic fibrosis with the combination of hepatectomyand DENA.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目 国家 973 重大科学研究计划资助项目（2012CBA01305）

作者简介 李萌乾（1988-），男，硕士在读，研究方向：肝癌肝损伤模型；通信作者：尹海芳，E-mail：haifangyin@tmu.edu.cn。

更新日期/Last Update: 2014-11-21