子查询返回的值不止一个。当子查询跟随在 =、!=、<、<=、>、>= 之后，或子查询用作表达式时，这种情况是不允许的。 体外实验中缺氧对中性粒细胞凋亡的影响-《天津医科大学学报》
|本期目录/Table of Contents|

[1]呼沛然,李萌璐,杨 欢,等.体外实验中缺氧对中性粒细胞凋亡的影响[J].天津医科大学学报,2014,20(06):0.
　HU Pei-ran,LI Meng-lu,YANG Huan,et al. Effects of hypoxia on apoptosis of neutrophils in vitro [J].Journal of Tianjin Medical University,2014,20(06):0.
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体外实验中缺氧对中性粒细胞凋亡的影响(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
20卷
期数:
2014年06期
页码:
0
栏目:
基础医学
出版日期:
2014-11-20

文章信息/Info

Title:
 Effects of hypoxia on apoptosis of neutrophils in vitro
作者:
呼沛然; 李萌璐; 杨 欢; 姚 智; 高 颖
 

 
Author(s):
HU Pei-ran;  LI Meng-lu;  YANG Huan;  YAO Zhi;  GAO Ying

（Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin Key Laboratory of Cellular and Molecular Immunology, Key Laboratory of Educational Ministry, Tianjin 300070, China）
关键词:
中性粒细胞; 缺氧; 凋亡; TNFR1
Keywords:
 ; neutrophils;  hypoxia;  apoptosis;  TNFR1
分类号:
-
DOI:
-
文献标志码:
-
摘要:
目的：通过在缺氧条件下体外培养中性粒细胞，探讨缺氧对中性粒细胞凋亡的影响。方法：用全反式维甲酸诱导NB-4细胞分化获得中性粒细胞并建立缺氧模型，用流式细胞技术检测缺氧对中性粒细胞凋亡和坏死的影响以及缺氧后CD11b阳性细胞百分率的变化，用免疫荧光技术检测缺氧对中性粒细胞的细胞膜TNFR1表达强度的影响。结果：缺氧后中性粒细胞增殖减缓，凋亡和坏死受到抑制，细胞膜TNFR1表达下降，CD11b+细胞百分率稍有增高。结论：缺氧可以减缓中性粒细胞增殖，同时抑制中性粒细胞的凋亡。
Abstract:
Objective: To investigate the effects of hypoxia on apoptosis of neutrophils by maintaining neutrophils in hypoxia conditions in vitro. Methods: Neutrophils were prepared by all-trans retinoic acid （ATRA）-induced differentiation of NB-4 cells, and neutrophil hypoxia model was made in vitro. Flow cytometry was used to detect the effects of hypoxia on apoptosis and necrosis of neutrophils as well as the percentage of CD11b positive cells. Immunofluorescence assay was used to detect the effects of hypoxia on the cell membrane expression of TNFR1 of neutrophils. Results: In hypoxia conditions, proliferation of neutrophils was suppressed, apoptosis and necrosis of neutroplils and TNFR1 expression of cell membrane were down-regulated , and the proportion of CD11b positive cells was slightly increased. Conclusion: Both proliferation and apoptosis of neutrophils are down-regulated in hypoxia conditions.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金资助项目（31071217）；教育部留学回国人员科研启动基金（2008-890）作者简介 呼沛然（1988-），女，硕士在读，研究方向：中性粒细胞凋亡与溶酶体蛋白的关系；通信作者：高颖，E-mail:gaoying@tijmu.edu.cn。
更新日期/Last Update: 2014-11-20