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[1]周 彤,赵 倩.整合多组学数据筛选胰腺癌进展中的关键基因[J].天津医科大学学报,2024,30(02):132-137,156.[doi:10.20135/j.issn.1006-8147.2024.02.0132]
　ZHOU Tong,ZHAO Qian.Integrating multi-omics data to screen key genes in pancreatic cancer progression[J].Journal of Tianjin Medical University,2024,30(02):132-137,156.[doi:10.20135/j.issn.1006-8147.2024.02.0132]
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整合多组学数据筛选胰腺癌进展中的关键基因(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
30卷
期数:
2024年02期
页码:
132-137,156
栏目:
肿瘤疾病专题
出版日期:
2024-03-20

文章信息/Info

Title:
Integrating multi-omics data to screen key genes in pancreatic cancer progression
文章编号:
1006-8147（2024）02-0132-07
作者:
周 彤; 赵 倩
（天津医科大学基础医学院细胞生物学系，天津 300070）
Author(s):
ZHOU Tong; ZHAO Qian
（Department of Cell Biology，School of Basic Medical Sciences，Tianjin Medical University，Tianjin 300070，China）
关键词:
胰腺癌; 生物信息学分析; 枢纽基因
Keywords:
pancreas cancer; bioinformatics analysis; key genes
分类号:
R735.9
DOI:
10.20135/j.issn.1006-8147.2024.02.0132
文献标志码:
A
摘要:
目的：整合多组学数据筛选胰腺癌进展中的关键基因。方法：在GEO数据库中下载GSE149103数据集，对正常胰腺导管细胞系（HPNE）、原发性胰腺癌细胞系（PANC）以及转移胰腺癌细胞系（Capan）的基因表达及染色质开放性测序数据进行差异分析，并对差异基因进行GO和KEGG富集分析、PPI网络构建以及Hub基因筛选。结果：HPNE与PANC组筛选出1 640个差异表达基因、1 826个差异峰值区域关联基因；PANC与Capan之间筛选出4 286个差异表达基因、4 489个差异峰值区域关联基因。GO和KEGG富集分析显示两组差异基因均在轴突形成功能、MAPK信号通路和Hippo信号通路富集。最后通过PPI网络的构建与分析得到HPNE与PANC组关键基因集FN1、SOX2、SELPLG、GREM1、MEF2C、 CCR7、FMN1、COL6A3、NR5A2、CTNNA2；PANC与Capan组关键基因集KDR、IL6、MMP2、CXCR4、FLT1、PPARG、 SOX2、ETS1、GRIN2A、NTRK2。其中SOX2为两组共有关键基因。结论：SOX2可能在胰腺癌发生、发展及转移过程中发挥着重要作用。
Abstract:
Objective：To screen key genes in pancreatic cancer progression by integrating multi-omics data. Methods：The GSE149103 data set in the GEO database was downloaded，and differential analysis on the gene expression and chromatin access sequencing data of normal pancreatic cells（HPNE），primary pancreatic cancer cells（PANC） and metastatic pancreatic cancer cells（Capan） was performed. GO and KEGG enrichment analysis of differential genes were processed. The PPI network was constructed and Hub genes were screened. Results：Totally，1 640 differentially expressed genes and 1 826 genes associated with differential peak were screened out in HPNE and PANC group； 4 286 differentially expressed genes and 4 489 genes associated with differential peak were screened out in PANC and Capan group. The GO and KEGG enrichment analyses revealed that both groups of differentially expressed genes are enriched in axon morphogenesis，MAPK signaling pathway，and Hippo signaling pathway. Finally，through the construction and analysis of the PPI network，the key gene sets for the HPNE and PANC groups including FN1，SOX2，SELPLG，GREM1，MEF2C，CCR7，FMN1，COL6A3，NR5A2，and CTNNA2 were identified； and the key genes for the PANC and Capan groups including KDR，IL6，MMP2，CXCR4，FLT1，PPARG，SOX2，ETS1，GRIN2A，and NTRK2 were obtained. SOX2 was a common key gene in both groups. Conclusion：SOX2 may plays an important role in the occurrence，development，and metastasis of pancreatic cancer.

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备注/Memo

备注/Memo:
作者简介 周彤（1999-），女，硕士在读， 研究方向：肿瘤的表观基因组学；通信作者：赵倩， E-mail：zhaoq@tmu.edu.cn。
更新日期/Last Update: 2024-03-20