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[1]余 冬,冯 霜,李 俊,等.MiR-199a-3p在胰腺癌中的表达水平及生物信息学分析[J].天津医科大学学报,2019,25(02):119-123.
 YU Dong,FENG Shuang,LI Jun,et al.The expression of miR-199a-3p in pancreatic cancer and bioinformatics analysis[J].Journal of Tianjin Medical University,2019,25(02):119-123.
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MiR-199a-3p在胰腺癌中的表达水平及生物信息学分析(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
25
期数:
2019年02期
页码:
119-123
栏目:
基础医学
出版日期:
2019-03-20

文章信息/Info

Title:
The expression of miR-199a-3p in pancreatic cancer and bioinformatics analysis
文章编号:
1006-8147(2019)02-0119-05
作者:
余 冬冯 霜李 俊孙晋津
(天津医科大学第二医院肝胆胰外科,天津 300211)
Author(s):
YU DongFENG ShuangLI JunSUN Jin-jin
(Department of Hepatobiliary and pancreatic surgery Surgery, The Second Hospital,Tianjin Medical University,Tianjin 300211,China)
关键词:
miR-199a-3p胰腺癌生物信息学
Keywords:
miR-199a-3p pancreatic cancer Bioinformatics
分类号:
R735.9
DOI:
-
文献标志码:
A
摘要:
目的:探讨miR-199a-3p在胰腺癌中的表达水平及作为胰腺癌的诊断指标的意义,并进行生物信息学分析。方法:从GEO数据库收集胰腺癌和正常胰腺组织中miR-199a-3p的数据。汇总数据绘制受试者工作特征曲线,进行Meta分析,采用生物信息学方法预测miR-199a-3p的靶基因,并对靶基因进行GO功能富集分析和KEGG通路富集分析。结果:收集到6个GEO数据,4个数据的ROC曲线表现出显著的诊断意义,Meta分析结果显示miR-199a-3p在胰腺癌中表达水平高于正常胰腺组织,差异有统计学意义(P<0.001)。预测到221个miR-199a-3p潜在的靶基因,其功能主要富集在基因表达的调控,细胞代谢的调控,蛋白质域特异性结合等生物学过程(P<0.001),KEGG分析结果显示主要集中在PI3K-Akt通路、黏着斑、肌动蛋白细胞骨架调控、癌症通路、Hippo通路、鞘脂通路、ErbB通路、MAPK通路等通路(P<0.05)。结论:相比正常胰腺组织,miR-199a-3p在胰腺癌为高表达,可作为胰腺癌的一个潜在诊断指标,并可能在胰腺癌的发生发展中起到重要作用,为胰腺癌的研究和治疗提供了新的方向。
Abstract:
Objective: To investigate miR-199a-3p’s expression levels and diagnostic value in pancreatic cancer, and to conduct bioinformatics analysis. Methods:We gathered miR-199a-3p expression data of pancreatic cancer and normal pancreatic tissues by analysis of a public dataset from Gene Expression Omnibus (GEO) databases. We plotted ROC curve and conducted Meta-analysis using all collected data. Furthermore, the bioinformatics analysis of miR-199a-3p involved target prediction analysis, gene ontology enrichment and KEGG pathway enrichment. Results: A total of six GEO data were finally collected in this study, four of these data showed significant diagnostic value by conducting ROC curve,and Meta-analysis indicated that miR-199a-3p expression was significantly higher in pancreatic cancer by GEO data compared with normal pancreatic tissue (P<0.001). The 221 target genes were predicted as potential target genes for miR-199a-3p. The function of target genes was mostly enriched in the regulation of cell metabolism, regulation of gene expression, protein domain specific binding and other biological processes(P<0.001). KEGG analysis was mostly observed in enrichment pathway, such as PI3K-Akt signaling pathway, focal adhesion, actin cytoskeleton regulation, cancer pathway, Hippo signaling pathway, sphingolipid signaling pathways, ErbB signaling pathway, MAPK signaling pathway and other Signal pathways (P<0.05). Conclusion: MiR-199a-3p expression is significantly higher in pancreatic cancer compared with normal pancreatic tissues. It can be used as a potential diagnostic biomarker of pancreatic cancer, and it may play a vital role in the development of pancreatic cancer. Meanwhile, it could provide a research direction for the treatment of pancreatic cancer in the future.

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备注/Memo

备注/Memo:
作者简介 余冬(1993-),男,硕士在读,研究方向:普通外科;通信作者:孙晋津,E-mail:sun.jinjin@126.com。
更新日期/Last Update: 2019-04-25