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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

29卷

期数:

2023年03期

页码:

235-242

栏目:

生物信息学专题

出版日期:

2023-05-20

文章信息/Info

Title:

Regulatory networks of miRNA，lncRNA，circRNA，transcription factors and target genes in asthma

文章编号:

1006－8147（2023）03-0235-08

作者:

李昱剑; 阚璇

（天津医科大学总医院儿科，天津 300070）

Author(s):

LI Yu-jian; KAN Xuan

（Department of Pediatrics，General Hospital，Tianjin Medical University，Tianjin 300070，China）

关键词:

哮喘; ceRNA调控网络; lncRNA; circRNA; 转录因子; 靶向药物

Keywords:

asthma; ceRNA regulatory network; lncRNA; circRNA; transcription factor; targeted drug

分类号:

R562.2+5

DOI:

-

文献标志码:

A

摘要:

目的：筛选支气管哮喘的差异表达基因（DEGs）并构建相应的ceRNA调控网络。方法：从GEO数据库中筛选出两个数据集：GSE43696和GSE64913。用R软件的Limma包筛选DEGs并用ClusterProfiler包完成富集分析。利用Cytoscape、Tarbase、ENCORI、LncBase、Circad、AnimalTFDB、DGIdb等数据库构建miRNA、lncRNA、circRNA、转录因子和靶向药物与靶基因的调控网络。结果：共得到76个DEGs，其中44个基因上调，32个基因下调。富集分析显示，DEGs的功能和通路主要富集在宿主免疫系统和炎症方面，如组织特异性免疫反应、细胞杀伤、白细胞介素（IL）-17信号通路、抑制一氧化氮产生等。最后，预测并筛选出了2个miRNA、7个lncRNA、1个circRNA、8个转录因子和91种靶向药物，并构建了相应的调控网络。结论：在哮喘中，SNX13和7个lncRNA通过hsa-miR-19b-3p和hsa-miR-218-5p参与CLCA1等基因的表达调控。此外，SPI1等转录因子和他尼氟酯等药物也可能干预哮喘相关基因的表达和调控。

Abstract:

Objective： Screening for differentially expressed genes（DEGs） in bronchial asthma and construction of the corresponding ceRNA regulatory network. Methods：Two data sets from the Gene Expression Omnibus（GEO） database：GSE43696 and GSE64913 were screened. The DEGs were studied by Limma package of R software，and enrichment analysis was completed by ClusterProfiler package of R software. Subsequently，Cytoscape，Tarbase，ENCORI，LncBase，Circad，AnimalTFDB，DGIdb and other databases were used to construct regulatory networks of miRNA，lncRNA，circRNA，transcription factors（TFs），targeted drugs and target genes. Results：A total of 76 DEGs were obtained，among which 44 genes were up-regulated and 32 genes were down-regulated. Enrichment analysis showed that the functions and pathways of DEGs were mainly concentrated in the host immune system and inflammatory response，such as tissue specific immune response，cell killing，IL-17 signaling pathway，inhibition of nitric oxide production，etc. Finally，two miRNAs，seven lncRNAs，one circRNA，eight TFs and ninety-one targeted drugs were predicted and screened out，and the corresponding regulatory networks were constructed. Conclusion：In asthma，SNX13 and 7 lncRNAs were involved in gene expression and regulation through hsa-miR-19b-3p and hsa-miR-218-5p. In addition，transcription factors such as SPI1 and drugs such as talniflumate may also interfere with the expression and regulation of asthma-related genes.

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备注/Memo

备注/Memo:

基金项目 天津市医学重点学科（专科）建设项目（TJYX2DXK-068C）
作者简介 李昱剑（1997-），男，硕士在读，研究方向：呼吸、消化方向；通信作者：阚璇，E-mail：ashelykx@126.com。

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更新日期/Last Update: 1900-01-01