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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

27卷

期数:

2021年06期

页码:

623-627

栏目:

临床医学

出版日期:

2021-11-15

文章信息/Info

Title:

The effect of R848 on the expression of IFN-γ, IL-33 and IgE in the peripheral blood of children with asthma and its clinical significance

文章编号:

1006-8147（2021）06-0623-05

作者:

熊蕾蕾; 张松林; 张向峰; 张志英; 靳秀红

（郑州大学附属儿童医院、河南省儿童医院、郑州儿童医院南院区呼吸科，郑州 450000）

Author(s):

XIONG Lei-lei; ZHANG Song-lin; ZHANG Xiang-feng; ZHANG Zhi-ying; JIN Xiu-hong

（Department of Respiratory Diseases，Zhengzhou University Children′s Hospital，Henan Children′s Hospital，Zhengzhou Children′s Hospital，South District，Zhengzhou 450000，China）

关键词:

哮喘; 急性发作期; Toll样配体; R848

Keywords:

asthma; acute attack period; toll-like ligand; R848

分类号:

R725.6

DOI:

-

文献标志码:

A

摘要:

目的：检测R848对哮喘患儿外周血中干扰素-γ（IFN-γ）、白细胞介素-33（IL-33）和IgE表达的影响及临床意义。方法：收集2018年6月—2020年5月在我院接受治疗的急性发作期哮喘患者125例，临床缓解期患者40例，分离培养外周血单个核细胞（PBMC）。ELISA实验检测PBMC分泌IFN-γ、IL-33和IgE的水平，qRT-PCR检测急性发作期哮喘患者PBMC中Toll样受体（TLR）7/8 mRNA的相对表达水平，Pearson分析急性发作期哮喘患者IFN-γ、IL-33和IgE与TLR7/8 mRNA的相对表达水平的相关性。ELISA实验检测不同浓度R848（0 mg/L、0.1 mg/L、1 mg/L、10 mg/L、100 mg/L）刺激急性发作期哮喘患者PBMC 24 h后IFN-γ、IL-33和IgE的水平。分析R848 10 mg/L刺激急性发作期哮喘患者PBMC细胞6 h、12 h、24 h、36 h、48 h后IFN-γ、IL-33和IgE水平的变化。结果：急性发作期哮喘患者外周血中PBMC分泌IFN-γ的水平低于临床缓解期，分泌IL-33和IgE的水平高于临床缓解期（均P<0.05）；IL-33水平与TLR7/8 mRNA相对表达水平呈负相关（r=-0.887，P<0.001；r=-0.910，P<0.001），IFN-γ水平与TLR7/8 mRNA相对表达水平呈正相关（r=0.818，P<0.001；r=0.808，P<0.001），IgE水平与TLR7 /8 mRNA相对表达水平呈负相关（r=-0.850，P<0.001；r=-0.838，P<0.001）。R848刺激PBMC细胞24 h后，IL-33和IgE水平在R848 1 mg/L时降低至R848 10 mg/L后趋于平稳（F=56.231、42.310，均P<0.05）。IFN-γ水平在R848 0.1 mg/L时逐渐升高至R848 10 mg/L后趋于平稳（F=62.352，P<0.05）。R848 10 mg/L刺激PBMC 6~48 h，IL-33和IgE水平在12 h时逐渐降低至36 h后趋于平稳（F=32.658、36.521，均P<0.05），IFN-γ在12 h时逐渐增加至36 h后趋于平稳（F=42.321，P<0.05）。结论：R848可抑制急性发作期患者外周血中PBMC分泌IL-33和IgE，促进IFN-γ的分泌，具有一定的抗炎作用，有望应用于哮喘的治疗。

Abstract:

Objective： To detect the effect of R848 on the expression of gamma interferon（IFN-γ），Interleukin-33（IL-33） and IgE in the peripheral blood of children with asthma and its clinical significance. Methods: A total of 125 patients with acute-onset asthma and 40 patients with clinical remission who received treatment in our hospital from June 2018 to May 2020 were collected，and peripheral blood mononuclear cells（PBMC） were isolated and cultured.ELISA test detected the levels of IFN-γ，IL-33 and IgE secreted by PBMC，qRT-PCR detected the relative expression level of toll-like receptor（TLR）7/8 mRNA in PBMC of acute-onset asthma patients，and Pearson detected the correlation between IFN-γ，IL-33 and IgE with the relative expression levels of TLR7/8 mRNA in patients with acute asthma.ELISA experiments were used to detect the levels of IFN-γ，IL-33 and IgE after stimulating PBMC of acute-onset asthma patients with different concentrations of R848（0 mg/L，0.1 mg/L，1 mg/L，10 mg/L，100 mg/L）. The changes of IFN-γ，IL-33 and IgE levels after 6 h，12 h，24 h，36 h，48 h stimulating PBMC by R848 10 mg/L in acute asthma patients were determinated. Results: The level of IFN-γ secreted by PBMC in peripheral blood of patients with acute asthma was lower than that in clinical remission，and the level of IL-33 and IgE secreted was higher than that of clinical remission（all P<0.05）. The level of IL-33 was negatively correlated with the relative expression level of TLR7/8 mRNA（r=-0.887，P<0.001; r=-0.910，P<0.001），and the level of IFN-γ was positively correlated with the relative expression level of TLR7/8 mRNA（r=0.818，P<0.001；r=0.808，P<0.001），the IgE level was negatively correlated with the relative expression level of TLR7/8 mRNA（r=-0.850，P<0.001；r=-0.838，P<0.001）.After R848 stimulated PBMC cells for 24 h，IL-33 and IgE gradually decreased from R848 1 mg/L to R848 10 mg/L and then stabilized（F=56.231，42.310，both P<0.05）. The level of IFN-γ gradually increased from R848 0.1 mg/L to R848 10 mg/L and then stabilized（F=62.352，P<0.05）. R848 10 mg/L stimulated PBMC for 6~48 h，IL-33 and IgE levels gradually decreased from12 h to 36 h and then stabilized（F=32.658，36.521，both P<0.05），and IFN-γ gradually increased from 12 h to 36 h and then stabilized（F=42.321，P<0.05）. Conclusion: R848 can inhibit the secretion of IL-33 and IgE from PBMC in the peripheral blood of patients with acute attacks，promote the secretion of IFN-γ，and has a certain anti-inflammatory effect. R848 is expected to be used in the treatment of asthma.

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备注/Memo

备注/Memo:

基金项目 河南省医学科技攻关计划联合共建项目（LHGJ20190977） 作者简介 熊蕾蕾（1989-），女，主治医师，硕士，研究方向：小儿呼吸疾病；通信作者: 靳秀红，E-mail：glgmyy01234@126.com。

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更新日期/Last Update: 2021-11-15