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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

27卷

期数:

2021年05期

页码:

439-445,481

栏目:

网络药理学专题

出版日期:

2021-09-10

文章信息/Info

Title:

Study on antidepressant mechanism of Ziziphi Spinosae Semen alkaloid based on network pharmacology

文章编号:

1006-8147(2021)05-0439-08

作者:

孙丹晨; 王志慧; 乔卫

（天津医科大学药学院天然药物学系，天津³000⁷0）

Author(s):

SUN Dan-chen; WANG Zhi-hui; QIAO Wei

（Department of Natural Medicines，College of Pharmacy，Tianjin Medical University，Tianjin 300070，China）

关键词:

酸枣仁生物碱; 网络药理学; 抗抑郁; GO分析; KEGG分析

Keywords:

ZSS alkaloids; network pharmacology; antidepressant; GO analysis; KEGG analysis

分类号:

R961+R749.4

DOI:

-

文献标志码:

A

摘要:

目的：采用网络药理学方法预测酸枣仁生物碱抗抑郁的作用机制。方法：首先挖掘相关的数据库如TCMSP、TCMDatabase@Taiwan、Drug Bank、Pharm Mapper、DAVID等，获得酸枣仁生物碱的主要成分和生物碱抗抑郁的潜在作用靶点，并对潜在靶点进行KEGG通路和GO分析。进一步采用软件Cytoscape构建并分析“成分-靶点-通路”生物网络图，最终从整体角度预测酸枣仁生物碱抗抑郁的作用机制。结果：酸枣仁生物碱抗抑郁的主要成分包括荷叶碱（nuciferine）、原荷叶碱（nornuciferine）、右旋衡州乌药碱（coclaurine）、木兰花碱（magnoflorine），主要作用于钙调蛋白（CALM1）、表皮生长因子受体（EGFR）、糖原合成酶激酶-3β（GSK3B）、视黄酸受体（RXRA）等靶点，涉及并影响磷脂酰肌醇3激酶-蛋白激酶B（PI3K-Akt）、钙离子等信号通路以及丝裂原活化蛋白激酶（MAPK）级联、蛋白结合等生物进程，发挥抗抑郁作用。结论：初步预测获得酸枣仁生物碱抗抑郁作用机制，多成分-多靶点-多通路的特点符合中医整体综合作用理念。

Abstract:

Objective： To explore the anti-depression mechanism of total alkaloids from Ziziphi Spinosae Semen（ZSS） based on network pharmacology. Methods: First，major components and potential anti-depressant targets of ZSS alkaloids were obtained by mining relevant databases such as TCMSP，TCMDatabase@Taiwan，Drug Bank，Pharm Mapper，DAVID，etc. KEGG pathway and GO analysis were performed on the potential targets.Furthermore，the software Cytoscape was used to construct and analyze the biological network diagram of "component-target-pathway". Finally，the anti-depressive mechanism of ZSS alkaloids was predicted from an overall perspective. Results: It was predicted that the main anti-depressant components of ZSS alkaloids included nuciferine，nornuciferine，coclaurine and magnoflorine，which played an anti-depressant role mainly by acting on the targets of calmodulin-1（CALM1），epidermal growth factor receptor（EGFR），glycogen synthase kinase-3 beta（GSK3B） and retinoic acid receptor RXR-alpha（RXRA），influencing phosphatidylinositol 3-kinase-protein kinase B（PI3K-Akt） and calcium signaling pathways as well as mitogen-activated protein kinase（MAPK）cascade and protein binding biological processes. Conclusion: The antidepressant mechanism of ZSS alkaloids is preliminarily predicted，and the characteristics of multi-component，multi-target and multi-pathway are consistent with the concept of integrated action of traditional Chinese medicine.

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备注/Memo

备注/Memo:

基金项目 天津市自然科学基金重点项目（17JCZDJC33200）
作者简介 孙丹晨（1997-），女，硕士在读，研究方向：中药药效物质机制研究；通信作者：乔卫，E-mail：qiaowei@tmu.edu.cn。

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更新日期/Last Update: 2021-09-01