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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

24卷

期数:

2018年03期

页码:

185-187

栏目:

出版日期:

2018-05-20

文章信息/Info

Title:

Using DNA tetrahedron as nanocarrier to study the synergistic effects of CpG on Melan-A peptide

作者:

和晨辰¹; 尹泽清²; 孙丽范¹; 于露³; 洪恩律³; 石文琦³; 李海东²; 沈万秋¹

天津医科大学1.药学院；2. 基础医学院；3.公共卫生学院，天津 300070

Author(s):

HE Chen-chen¹;  YIN Ze-qing²;  SUN Li-fan¹;  YU Lu³;  HONG En-lü³;  SHI Wen-qi³;  LI Hai-dong²;  SHEN Wan-qiu¹

1.School of Pharmacy; 2. School of Basic Medical Sciences; 3. School of Public Health, Tianjin Medical University , Tianjin 300070，China

关键词:

DNA四面体; Melan-A抗原肽; CpG; 协同作用; 小鼠

Keywords:

DNA tetrahedron;  Melan-A antigen peptide;  CpG;  synergistic effects;  mouse

分类号:

R9

DOI:

-

文献标志码:

A

摘要:

目的：通过将Melan-A抗原肽与CpG连接在DNA四面体上，并控制肽链与CpG之间的距离和CpG的个数，研究CpG对具有免疫原性的Melan-A抗原肽的协同作用。方法：将特定设计合成好的四面体-肽、四面体-肽-CpG（Ｉ）、四面体-肽-CpG（ＩＩ）注射到小鼠体内，运用ELISA法检测小鼠血清中IL-6的浓度。结果：相应产生的IL-6的浓度为9.238 pg/mL、17.150 pg/mL、18.865 pg/mL。结论：CpG增强了Melan-A抗原肽的免疫作用，并且CpG个数越多增强效果越好。

Abstract:

Objective: To study the synergistic effects of CpG oligodeoxynucleotides and Melan-A antigen peptide by adding a Melan-A peptide and varied numbers of CpGs to the specified positions of a DNA tetrahedron nanostructure. Methods: Special designed DNA tetrahedron-peptide, DNA tetrahedron-peptide-CpG(I) and DNA tetrahedron-peptide-CpG (II) nanostructures were prepared and injected into mice. ELISA method was then used to detect the level of cytokine interleukin(IL) -6 in mice serums. Results: The concentrations of IL-6 were 9.238 pg/mL, 17.150 pg/mL, and 18.865 pg/mL, respectively. Conclusion: CpG could enhance the immunization effect of the Melan-A antigen peptide, and the effect becomes greater with increased number of CpGs added to the DNA tetrahedron nanostructure.

参考文献/References:

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相似文献/References:

备注/Memo

备注/Memo:

文章编号 1006-8147(2018)03-0185-03
基金项目 国家自然科学基金面上项目基金资助（21373151）
作者简介 和晨辰（1992-），女，硕士在读， 研究方向： DNA纳米结构及其在生物医学方面的应用；通信作者：沈万秋，E-mail：wshen@tmu.edu.com。

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更新日期/Last Update: 2018-05-20