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[1]侯肖肖,孙丽范,樊明玥,等.DNA为模板的纳米银簇探针检测肿瘤细胞高表达microRNA-21[J].天津医科大学学报,2018,24(03):188-191.
 HOU Xiao-xiao,SUN Li-fan,FAN Ming-yue,et al.Detection of microRNA-21 up-expression in tumor cells with DNA-templated silver nanocluster probe[J].Journal of Tianjin Medical University,2018,24(03):188-191.
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DNA为模板的纳米银簇探针检测肿瘤细胞高表达microRNA-21(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
24
期数:
2018年03期
页码:
188-191
栏目:
论著(基础医学)
出版日期:
2018-05-20

文章信息/Info

Title:
Detection of microRNA-21 up-expression in tumor cells with DNA-templated silver nanocluster probe
作者:
侯肖肖1孙丽范1樊明玥2孙艳华1
天津医科大学1. 药学院;2.公共卫生学院,天津300070
Author(s):
HOU Xiao-xiao1 SUN Li-fan1 FAN Ming-yue2 SUN Yan-hua1
1. School of Pharmacy; 2. School of Public Health, Tianjin Medical University, Tianjin 300070, China
关键词:
肿瘤标志物纳米银簇microRNA-21荧光性质
Keywords:
tumor markers AgNCs microRNA-21 fluorescence properties
分类号:
R9
DOI:
-
文献标志码:
A
摘要:
目的:设计由DNA模板引导制备的纳米银簇探针,实现对肿瘤细胞中高表达的microRNA-21的定性和定量检测。方法:利用荧光光谱、紫外-可见吸收光谱、圆二色谱以及高分辨电镜表征技术,探究DNA引导合成纳米银簇的性质。通过计算血清回收率,验证探针对microRNA-21的检测效率。结果:以DNA为模板合成的纳米银簇探针能成功检测肿瘤细胞高表达的microRNA-21。结论:建立了microRNA-21的检测方法,并为临床检测肿瘤标志物提供了思路。
Abstract:
Objective: To detect the up-expressed microRNA-21 in tumor cells qualitatively and quantitatively using designed DNA-templated silver nanoclusters(DNA/AgNCs) probe. Methods: Fluorescence spectroscopy, UV-Vis absorption spectroscopy, circular dichroism spectroscopy and high-resolution electron microscopy were employed to study properties of DNA/AgNCs probes. The detection efficiency for microRNA-21 was tested by the calculating serum recovery. Results: The up-expressed microRNA-21 in tumor cells was detected by DNA/AgNCs probe successfully. Conclusion: A method for the detection of microRNA-21 is established and it could be used for clinical detection of tumor biomarkers.

参考文献/References:

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备注/Memo

备注/Memo:
文章编号 1006-8147(2018)03-0188-04
基金项目 国家自然科学基金资助项目(31200753)
作者简介 侯肖肖(1989-),女,硕士在读,研究方向:DNA调控纳米银簇应用于microRNA检测;通信作者:孙艳华,E-mail:sunyh@tmu.edu.cn。
更新日期/Last Update: 2018-05-20