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[1]陈文博,寇亚芬,张引国,等.贝沙罗汀拮抗Aβ25-35诱导的海马CA1区锥体神经元谷氨酸能突触传递的抑制效应[J].天津医科大学学报,2017,23(04):285-289.
　CHEN Wen-bo,KOU Ya-fen,ZHANG Yin-guo,et al.Antagonistic effect of bexarotene on Aβ25-35 -Induced inhibiting action in glutamatergic synaptic transmission of hippocampal CA1 pyramidal neurons[J].Journal of Tianjin Medical University,2017,23(04):285-289.
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贝沙罗汀拮抗Aβ25-35诱导的海马CA1区锥体神经元谷氨酸能突触传递的抑制效应(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
23卷
期数:
2017年04期
页码:
285-289
栏目:
基础医学
出版日期:
2017-07-02

文章信息/Info

Title:
Antagonistic effect of bexarotene on Aβ25-35 -Induced inhibiting action in glutamatergic synaptic transmission of hippocampal CA1 pyramidal neurons
文章编号:
1006-8147(2017)04-0285-05
作者:
陈文博1; 寇亚芬2; 张引国3; 赵景霞1; 张 玲1
（1.天津医科大学生理学与病理生理学系，天津 300070；2.天津医科大学一中心临床学院放射科，天津 300192；3.武警后勤学院生理学与病理生理学教研室，天津300309）
Author(s):
CHEN Wen-bo1;  KOU Ya-fen2;  ZHANG Yin-guo3;  ZHAO Jing-xia1;  ZHANG Ling1
（1. Department of Physiology and Pathophysiology, Tianjin Medical University, Tianjin 300070, China; 2. Department of Radiology, The First Center Clinic College, Tianjin Medical University, Tianjin 300192, China; 3. Department of Physiology and Pathophysiology, Logistics University of Chinese People’s Armed Police Force, Tianjin 300309, China）
关键词:
阿尔茨海默病; β淀粉样蛋白; 贝沙罗汀; 自发性兴奋性突触后电流; 微小性兴奋性突触后电流
Keywords:
Alzheimer’s disease;  amyloid beta-protein;  Bexarotene;  spontaneous excitatory postsynaptic currents;  miniature excitatory postsynaptic currents
分类号:
R338.8
DOI:
-
文献标志码:
A
摘要:
目的:初步探讨β淀粉样蛋白Aβ25-35损伤海马CA1区兴奋性突触的靶点及贝沙罗汀的可能拮抗效应。方法 以出生7～14 d Wistar大鼠海马脑片为研究对象，采用全细胞膜片钳技术，在电压钳模式下记录大鼠海马脑片CA1区锥体细胞自发兴奋性突触后电流（sEPSCs）和微小兴奋性突触后电流（mEPSCs），并分析不同组神经元sEPSCs和mEPSCs幅度及频率的差异。结果:与对照组相比，经Aβ25-35（1 μmol/L）处理后，海马神经元sEPSCs与mEPSCs平均幅度和平均频率都显著降低（均P< 0.05）。向Aβ25-35处理过的海马脑片中加入贝沙罗汀（5μmol/L）后，sEPSCs与mEPSCs平均幅度和平均频率较Aβ25-35组都显著提高（均P< 0.05）。贝沙罗汀处理组sEPSCs与mEPSCs平均频率和平均幅度与对照组水平已无显著性差异（均P> 0.05）。结论:Aβ25-35可作用于CA1区，导致海马锥体神经元兴奋性突触后电位降低，造成突触功能损伤，而贝沙罗汀通过作用于突触前和突触后位点拮抗Aβ25-35的损伤效应。
Abstract:
Objective: To explore the damage effect target of?? Aβ25-35??on hippocampal CA1 excitatory synapses and the possible antagonistic effect of bexarotene. Methods: Postnatal 7-14-day-old Wistar rats were used as the research subjects. Using a whole-cell patch clamp technique, and in voltage-clamp mode, we recorded spontaneous excitatory postsynaptic currents (sEPSCs) and miniature excitatory postsynaptic currents (mEPSCs) of CA1 pyramidal neurons in hippocampal slices. The changes of the amplitude and frequency of the sEPSCS and mEPSCs in each group were analyzed. Results: After Aβ25–35 (1 umol/L) application, the average amplitude and frequency of sEPSCs and mEPSCs were significantly reduced compared with the control group (P< 0.05). Following application of bexarotene (5 umol/L) to Aβ25-35 group, the average amplitude and frequency of sEPSCs and mEPSCs were significantly increased compared with the Aβ25–35 group(P< 0.05); and compared with the control group, the difference was not statistically significant (P> 0.05). Conclusion: Aβ25-35 has a damage effect on CA1 pyramidal neurons, resulting in the decrease of excitatory postsynaptic potential and synaptic function damage, while bexarotene has a presynaptic and postsynaptic site of action to antagonize Aβ25-35-induced synaptic dysfunction in hippocampal CA1 pyramidal neurons.

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金资助项目（81271224）；天津市自然科学基金资助项目（15JCYBJC25400）；教育部留学回国人员科研启动基金资助项目

作者简介 陈文博（1991-），男，硕士在读，研究方向：生理学；

通信作者： 张玲，E-mail： zhanglsl@tmu.edu.cn; 赵景霞，zhaojingxia@tmu.edu.cn。
更新日期/Last Update: 2017-06-30