|本期目录/Table of Contents|

[1]孙凤仙,徐淑梅.H102-BD经鼻腔给药后对双转基因AD小鼠行为学及脑内APP和Aβ蛋白表达的影响[J].天津医科大学学报,2017,23(05):385-388.
 SUN Feng-xian,XU Shu-mei.Effect of H102-BD on behavior and the expression of APP and Aβ in PAP double transgenic AD mice after intranasal administration[J].Journal of Tianjin Medical University,2017,23(05):385-388.
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H102-BD经鼻腔给药后对双转基因AD小鼠行为学及脑内APP和Aβ蛋白表达的影响(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
23
期数:
2017年05期
页码:
385-388
栏目:
基础医学
出版日期:
2017-09-20

文章信息/Info

Title:
Effect of H102-BD on behavior and the expression of APP and Aβ in PAP double transgenic AD mice after intranasal administration
文章编号:
1006-8147(2017)05-0385-04
作者:
孙凤仙徐淑梅
(天津医科大学生理学与病理生理学系,天津 300070)
Author(s):
SUN Feng-xian XU Shu-mei
( Department of Physiology and Pathophysiology, Tianjin Medical University, Tianjin 300070, China)
关键词:
H102-BD' target="_blank" rel="external">">H102-BD鼻腔给药Morris水迷宫阿尔茨海默病小鼠
Keywords:
H102-BD' target="_blank" rel="external">">H102-BDintranasal administrationmorris water mazeAlzheimer’s diseasemice
分类号:
R33
DOI:
-
文献标志码:
A
摘要:
?目的:观察β片层阻断肽H102-BD经鼻腔给药后对PAP双转基因AD小鼠行为学、脑内APP及Aβ蛋白表达的影响。方法:将PAP转基因小鼠随机分为模型组和H102-BD给药组,并设同月龄同背景C57BL/6J小鼠为正常对照组。鼻腔给药4周后行Morris水迷宫实验,利用免疫组织化学方法和Western blot方法测定小鼠脑内APP和Aβ蛋白的表达。结果:(1)Morris水迷宫结果显示鼻腔给予H102-BD后AD模型鼠的空间记忆能力有了明显的提高。(2)免疫组化及Western blot结果显示,H102-BD可显著降低AD模型鼠脑内APP及Aβ蛋白表达。结论:β片层阻断肽H102-BD经鼻腔给药后对AD有一定的治疗作用。
Abstract:

Objective: To investigate the effect of H102-BD on behavior and the expression of APP and Aβ in PAP transgenic AD mice after intranasal administration. Methods: The PAP transgenic mice were randomly divided into model group and H102-BD treatment group, and a group of C57BL/6J mice with the same age and background was set as normal. After intranasal administration for four weeks, the ability of spatial reference memory was tested by Morris Water Maze, and then the immunohistochemical stain and Western blot were carried out to detect the content of APP and Aβ in mice brain. Results: (1) The memory of H102-BD group was significantly improved compared with that in model group by the test of Morris Water Maze. (2) The contents of APP and Aβ were significantly decreased in H102-BD group compared with those in model group by the test of immunohistochemical stain and Western blot. Conclusion: β-sheet breaker H102-BD may be an effective therapeutic strategy for AD after intranasal administration.


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参考文献/References:

[1] Lalatsa A, Schatzlein A G, Uchegbu I F. Strategies to deliver peptide drugs to the brain.[J]. Mol Pharm, 2014,11(4):1081

[2] Gao H, Pang Z, Jiang X. Targeted delivery of nano-therapeutics for major disorders of the central nervous system[J]. Pharm Res, 2013,30(10):2485

[3] Lin L X, Bo X Y, Tan Y Z, et al. Feasibility of β-sheet breaker peptide-H102 treatment for Alzheimer’s disease based on β-amyloid hypothesis[J]. PloS One. 2014, 9(11):e112052

[4] Ghalamfarsa G, Hojjat-Farsangi M, Mohammadnia-Afrouzi M, et al. Application of nanomedicine for crossing the blood–brain barrier: theranostic opportunities in multiple sclerosis[J]. J Immunotoxicol, 2016,13(5):603

[5] Schaefer M L, B?ttger B, Silver W L, et al. Trigeminal collaterals in the nasal epithelium and olfactory bulb: a potential route for direct modulation of olfactory information by trigeminal stimuli[J]. J Comp Neurol, 2002, 444(3):221

[6] Hardy J, Selkoe D J. The amyloid hypothesis of Alzheimer’s disease: progress and

problems on the road to therapeutics[J]. Science, 2002, 297(5580):353

[7] 王冰艳,孙凤仙,林来祥,等. β片层阻断肽H102对PAP小鼠脑内ERK信号转导通路的影响[J]. 天津医药,2014, 42(7):650

[8] Graft C L, Pollack G M. Nasal drug administration: potential for targeted central nervous system delivery[J]. J Pharm Sci, 2005, 94(6): 1187

[9] Illum L. Nasal drug delivery-possibilities, problems and solutions[J]. J Control Release, 2003 ,87(1-3):187

[10] Mittal D, Ali A, Md S, et al. Insights into direct nose to brain delivery: currentstatus and future perspective[J]. Drug Deliv, 2014, 21(2):75

[11] Ryan S D, Whitehead S N, Swayne L A, et al. Amyloid-β42 signals tau hyperphosphorylation and compromises neuronal viability by disrupting alkylacylglycerophosphocholine metabolism[J]. Proc Natl Acad Sci, 2009, 106(49): 20936

[12] Huang J, Chen Y J, Bian W H, et al. Unilateral amyloid-beta25-35 injection into the rat amygdala increases the expressions of aberrant tau phosphorylation kinases[J]. Chin Med J (Engl), 2010, 123(10):1311

[13] Llorens-Marítin M, Jurado J, Hernández F, et al. GSK-3β, a pivotal kinase in

Alzheimer disease[J]. Front Mol Neurosci, 2014, 7(46):1

[14] De Felice F G, Wu D, Lambert M P, et al. Alzheimer’s disease-type neuronal tau hyperphosphorylation induced by A beta oligomers[J]. Neurobiol Aging, 2008, 29(9):1334

[15] Yu X, Wang L N, Du Q M, et al. Akebia Saponin D attenuates amyloid β-induced cognitive deficits and inflammatory response in rats: involvement of Akt/NF-κB pathway[J]. Behav Brain Res, 2012, 235(2):200

[16] Saha R N, Pahan K. Regulation of inducible nitric oxide synthase gene in glial cells[J]. Antioxid Redox Signal, 2006, 8(5/6):929

[17] Frenzel D, Glück J M, Brener O, et al. Immobilization of homogeneous monomeric, oligomeric and fibrillar Aβ species for reliable SPR measurements[J]. PLoS One, 2014, 9(3):e89490

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备注/Memo

备注/Memo:

基金项目 国家科技重大专项基金资助(2009ZX09103-029);天津市科技计划项目(16YFZCSY01000)

作者简介 孙凤仙(1984-),女,助理实验师,硕士,研究方向:生理学;

通信作者:徐淑梅,E-mail:xushm@tmu.edu.cn

更新日期/Last Update: 2017-09-20