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[1]彭武建,陈文标.Alport综合征患者尿肾状杆细胞诱导成多能干细胞系的建立[J].天津医科大学学报,2017,23(02):128-131.
　PENG Wu-jian,CHEN Wen-biao.Establishment of pluripotent stem cells line induced by? renal tubular cells?in patient with Alport syndrome[J].Journal of Tianjin Medical University,2017,23(02):128-131.
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Alport综合征患者尿肾状杆细胞诱导成多能干细胞系的建立(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
23卷
期数:
2017年02期
页码:
128-131
栏目:
基础医学
出版日期:
2017-03-20

文章信息/Info

Title:
Establishment of pluripotent stem cells line induced by? renal tubular cells?in patient with Alport syndrome
文章编号:
1006-8147(2017)02-0128-04
作者:
彭武建1; 陈文标2
（1.深圳市第三人民医院肾脏内科，深圳 518112；2.深圳市光明新区人民医院肾脏内科，深圳 518107）
Author(s):
PENG Wu-jian1;  CHEN Wen-biao2
(1.Department of Nephrology, The Third People′Hospital of Shenzhen, Shenzhen 518112，China; 2. Department of Nephrology, Shenzhen Guangming New District People′Hospital, Shenzhen 518107，China)
关键词:
Alport综合征; 诱导多能干细胞; 尿肾状杆细胞
Keywords:
Alport syndrome;  induced pluripotent stem cells;  renal tubular cells

分类号:
Q343
DOI:
-
文献标志码:
A
摘要:
目的：从Alport综合征（AS）患者尿肾状杆细胞成功诱导成多能干细胞（iPSCs）。掌握建立iPSCs细胞系的技术与方法。方法：利用慢性病毒介导4种转录因子（Oct4，SOX2，Klf4，c-Myc）诱导从尿液中分离出的尿肾状杆细胞，建立诱导成干细胞样的克隆。挑选成功诱导的iPSCs克隆进行扩大培养，并对克隆进行免疫荧光检测，克隆形态观察，RT-PCR分析iPSCs细胞相关基因，体外三胚层分析及核型鉴定。结果：成功地从尿肾状杆细胞诱导成iPSCs，所获得的iPSCs可以表达人胚胎干细胞多能性分子标记，具有体外分化3个胚层的能力。在培养过程中能始终保持核型的稳定，并且能自我增殖、更新、分化。结论：Alport综合征患者尿液中的尿肾状杆细胞成功诱导为iPSCs，为今后研究AS提供了良好的细胞模型。
Abstract:
Objective: To generate Induced Pluripotent Stem Cells (iPSCs) from renal tubular cells of patient with Alport syndrome (AS), and to explore the technology and methods of establishment of iPSCs line. Methods: IPSCs were generated from renal tubular cells via ectopic of four transcription factors (Oct4 ， SOX2 ， Klf4 ， c-Myc ). The iPSCs were picked up from colonies to further enlarge cultivation after the successful establishment of iPSCs. Typical colonies were identified by immunofluorescence, colony morphology, RT-PCR analysis, differentiation of three germs layer and karyotype analysis. Results: IPSCs were successfully generated from renal tubular cells. The iPSCs showed characteristics similar to those of human embryonic stem cell (HESc), such as the molecule markers of HESc, differentiation into cells types of the three germ layer, the stability of karyotype and the ability of self-renewal, proliferation and differentiation. Conclusion: iPSCs could be generated from renal tubular cells of patent of AS, which could be used as the cellular model for further research of AS.

参考文献/References:

[1]彭静, 黄聪, 蒋思文. 诱导多潜能干细胞(iPSCs)的临床应用进展 [J]. 科学通报, 2014（9）: 763

[2]褚海涛，李晓蕾，贾心善．诱导多能干细胞的研究现状及展望**★[J]．中国组织工程研究，2013，23（23）：4340

[3]Zhou T , Benda C, Duzinger S, et al. Generation of induced pluripotent stem cells from urine[J]. J Am Soc Nephrol, 2011, 22(7): 1221

[4]董程, 陈斯琦, 张焱琴, et al. X-连锁Alport综合征的听力表现与皮肤α5(Ⅳ)胶原表达的关系 [J]. 中华耳科学杂志, 2014,11 （2）: 275

[5]李玉峰, 卫敏江, 吴伟岚, et al. 儿童肾活检977例病理及临床分析 [J]. 上海交通大学学报(医学版), 2014, 34（3）: 333

[6]陈楠. 重视遗传性肾脏疾病的筛查与诊治 [J]. 中国实用内科杂志, 2014, 34（3）: 217

[7]Takahashi K, Yamanaka S. A developmental framework for induced pluripotency[J]. Development, 2015, 142(19): 3274

[8]Bahadur G, Morrison M, Machin L. Beyond the ’embryo question’: human embryonic stem cell ethics in the context of biomaterial donation in the UK[J]. Reprod Biomed Online, 2010, 21(7): 868

[9]Gauthier M, Maury Y, Peschanski M, et al. Human pluripotent stem cells for genetic disease modeling and drug screening[J]. Regen Med, 2011, 6(5): 607

[10]Telliam G, Féraud O, Griscelli F, et al. Generation of an induced pluripotent stem cell line from a patient with chronic myeloid leukemia (CML) resistant to targeted therapies[J]. Stem Cell Res, 2016, 17(2): 235

[11]Wan W, Cao L, Kalionis B, et al. Applications of induced pluripotent stem cells in studying the neurodegenerative diseases[J]. Stem Cells Int, 2015，2015: 382530

[12]Inoue H, Nagata N, Kurokawa H, et al. iPS cells: a game changer for future medicine[J]. EMBO J, 2014, 33(5): 409

[13]Song B, Niclis J, Alikhan M, et al. Generation of induced pluripotent stem cells from human kidney mesangial cells[J]. J Am Soc Nephrol, 2011, 22(7): 1213

[14]Chen Y, Luo R, Xu Y, et al. Generation of systemic lupus erythematosus-specific induced pluripotent stem cells from urine[J]. Rheumatol Int, 2013, 33(8): 2127

相似文献/References:

[1]王欣,张碧丽.误诊为薄基底膜肾病的Alport综合征1例[J].天津医科大学学报,2022,28(06):675.

备注/Memo

备注/Memo:
基金项目 广东省深圳市科技计划基金资助项目（JXY20140416122812045）

作者简介 彭武建（1976-），男，主治医师， 博士，研究方向：主要从事肾脏病学基础及临床研究；通信作者：陈文标 E-mail：chanwenbiao@sina.com 。



更新日期/Last Update: 2017-03-28