«上一篇/Previous Article|本期目录/Table of Contents|下一篇/Next Article»

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

21卷

期数:

2015年06期

页码:

484-487

栏目:

基础医学

出版日期:

2015-11-20

文章信息/Info

Title:

Effect of rosiglitazone on the expression of CMKLR1 and Chemerin mRNA in the adipose tissue of diabetic rat

文章编号:

1006-8147（2015）06-0484-04

作者:

李 冰¹; 冯 凭²

(1.天津市儿童医院ICU,天津300134;2.天津医科大学总医院代谢病科,天津300070)

Author(s):

?LI Bing¹;  FENG Ping²

（1.Department of Intensive Care Unit, Tianjin Children Hospital,Tianjin300074，China;2.Department of Metabolism,General Hospital,Tianjin Medical University,Tianjin 300070,China）

关键词:

罗格列酮' target="_blank" rel="external">">罗格列酮; 糖尿病; 大鼠; Chemerin-CMKLR1; 基因表达

Keywords:

rosiglitazone;  diabetes;  rat;  Chemerin-CMKLR1;  gene expression

分类号:

R587.1

DOI:

-

文献标志码:

A

摘要:

目的：通过检测罗格列酮干预后CMKLR1及Chemerin在自发性2型糖尿病大鼠脂肪组织中表达水平，探讨Chemerin-CMKLR1通路在肥胖、胰岛素抵抗及2型糖尿病发病机制中的作用。方法：4周龄糖尿病大鼠30只，经相关实验至30周证实造模成功2型糖尿病大鼠20只，再随机分为罗格列酮干预组和模型组各10只，进行相关实验室指标检测，以实时荧光定量法检测脂肪组织CMKLR1及Chemerin基因的表达水平。结果：罗格列酮干预后大鼠各实验室指标明显改善，干预组大鼠网膜组织CMKLR1的表达明显低于模型组（P <0.01），干预后网膜及皮下脂肪组织中Chemerin的表达明显低于模型组（P <0.01）。罗格列酮干预后网膜脂肪细胞平均最大直径明显缩小（P<0.01），而皮下脂肪细胞平均最大直径无明显变化（P >0.05）。结论：Chemerin-CMKLR1通路成为治疗肥胖、胰岛素抵抗和2型糖尿病的新靶点。

Abstract:

Objective: To investigate the expression of CMKLR1 and Chemerin mRNA in subcutaneous and visceral adipose tissue of rats and discuss the intervention of Chemerin-CMKLR1 to involve in the pathogenesis of obesity, insulin resistance and type 2 diabetes. Methods: Thirty male rats of 4 weeks were selected. Twenty rats were proved with diabetes through experimental methods after 30 weeks. Models were separated into rosiglitazone group and diabetes group with ten rats in each group. Experiment dates were detected. The mRNA levels of CMKLR1 and Chemerin in adipose tissue by real-time PCR. Results: Experiments dates were improved after the intervention of rosiglitazone. The mRNA levels of CMKLR1 in visceral adipose tissue of rats in rosiglitazone group were lower than those in diabetes group(P<0.01).The mRNA levels of Chemerin of subcutaneous and visceral adipose tissue of rats in rosiglitazone group were lower than those in diabetes group (P<0.01). Maximum diameter in visceral adipocyte in rosiglitazone group decreased compared with that in diabetes group (p<0.01). Maximum diameter was of no difference in subcutaneous tissue between two groups (P >0.05). Conclusion: The intervention of the expression of CMKLR1 and Chemerin mRNA become new treatment target of obesity and insulin resistance and type 2 diabetes by rosiglitazone.

?

参考文献/References:

[1]Owman C, Nilsson C, Lolait S J. Cloning of cDNA encoding a putative chemoattractant receptor[J]. Genomics, 1996,37(2):187
[2]Zabel B A, Allen S J, Kulig P, et al. Chemerin activation by serine proteases of the coagulation, fibrinolytic, and inflammatory cascades[J]. J Biol Chem, 2005,280(41):34661
[3]Mori S, Kawano K, Hirashima T, et al. Relationships between diet control and the development of spontaneous type II diabetes and diabetic nephropathy in OLETF rats[J]. Diabetes Res Clin Pract, 1996,33(3):145
[4]Goralski K B, Mccarthy T C, Hanniman E A, et al. Chemerin, a novel adipokine that regulates adipogenesis and adipocyte metabolism[J]. J Biol Chem, 2007,282(38):28175
[5]Bozaoglu K, Bolton K, Mcmillan J, et al. Chemerin is a novel adipokine associated with obesity and metabolic syndrome[J]. Endocrinology, 2007,148(10):4687
[6]Ernst M C, Issa M, Goralski K B, et al. Chemerin exacerbates glucose intolerance in mouse models of obesity and diabetes[J]. Endocrinology, 2010,151(5):1998
[7]Roh S G, Song S H, Choi K C, et al. Chemerin--a new adipokine that modulates adipogenesis via its own receptor[J]. Biochem Biophys Res Commun, 2007,362(4):1013
[8]Yoshikawa H, Kihara Y, Taguchi M, et al. Role of TGF-beta1 in the development of pancreatic fibrosis in Otsuka Long-Evans Tokushima Fatty rats[J]. Am J Physiol Gastrointest Liver Physiol, 2002,282(3):G549
[9]Lee D H, Park D B, Lee Y K, et al. The effects of thiazolidinedione treatment on the regulations of aquaglyceroporins and glycerol kinase in OLETF rats[J]. Metabolism, 2005,54(10):1282
[10]Han S J, Kang E S, Hur K Y, et al. Rosiglitazone inhibits early stage of glucolipotoxicity-induced beta-cell apoptosis[J]. Horm Res, 2008,70(3):165
[11]Wittamer V, Grégoire F, Robberecht P, et al. The C-terminal nonapeptide of mature chemerin activates the chemerin receptor with low nanomolar potency[J]. J Biol Chem, 2004,279(11):9956
[12]Zabel B A, Silverio A M, Butcher E C. Chemokine-like receptor 1 expression and chemerin-directed chemotaxis distinguish plasmacytoid from myeloid dendritic cells in human blood[J]. J Immunol, 2005,174(1):244
[13]Hao F, Mu J W, Zhang H J, et al. Damage to vascular endothelial cells by high insulin levels is associated with increased expression of ChemR23, and attenuated by PPAR-gamma agonist, rosiglitazone[J]. Neuro Endocrinol Lett, 2015,36(1):
[14]Esteghamati A, Ghasemiesfe M, Mousavizadeh M, et al. Pioglitazone and metformin are equally effective in reduction of chemerin in patients with type?2 diabetes[J]. J Diabetes Investig, 2014,5(3):327
[15]Lefterova M I, Lazar M A. New developments in adipogenesis[J]. Trends Endocrinol Metab, 2009,20(3):107

相似文献/References:

[1]王 凊,车绪春.调节性T细胞获得及其免疫抑制作用的实验研究[J].天津医科大学学报,2015,21(05):393.
　WANG Qing,CHE Xu-chun.Study on iTreg cells obtaining method and its immunosuppression function [J].Journal of Tianjin Medical University,2015,21(06):393.
[2]陈晓鹏,胡永成,方 成,等.血红素氧化酶1在ADSCs增殖、凋亡和成骨分化的作用[J].天津医科大学学报,2015,21(05):379.
　CHEN Xiao-peng,HU Yong-cheng,FANG Cheng,et al. Effect of proliferation, apoptosis and osteogenic differentiation of ADSCs transduced with heme oxygenase-1? [J].Journal of Tianjin Medical University,2015,21(06):379.
[3]刘 磊,李彦生,俞浩楠,等.11C左旋间羟基麻黄素制备方法的优化及改进[J].天津医科大学学报,2015,21(05):441.
　LIU Lei,LI Yan-sheng,YU Hao-nan,et al.Optimization and improvement of production of (-)-[11C]meta-Hydroxyephedrine[J].Journal of Tianjin Medical University,2015,21(06):441.
[4]LI Yan-ping,CHAI Yan-fen.?连续血液滤过对严重脓毒症患者部分炎性指标及器官功能的影响 [J].天津医科大学学报,2015,21(06):491.
　LI Yan-ping,CHAI Yan-fen.?Effect of continuous hemofiltration on inflammatory mediators and organ function in severe sepsis[J].Journal of Tianjin Medical University,2015,21(06):491.
[5]孟昀,张华,于泳浩.右美托咪定对老年腹腔镜手术患者脑氧饱和度及术后认知功能的影响[J].天津医科大学学报,2016,22(01):66.
　MENG Yun,ZHANG Hua,YU Yong-hao?.Effect of dexmedetomidine on cerebral oxygen saturation and cognitive function in elder patients during laparoscopic surgery [J].Journal of Tianjin Medical University,2016,22(06):66.
[6]李宏亮,刘 彤,许 纲,等.红细胞分布宽度与阵发性非瓣膜性心房颤动射频消融复发的关系[J].天津医科大学学报,2016,22(02):104.
　LI Hong-liang,LIU Tong,XU Gang,et al.Association between RDW and atrial fibrillation recurrence after radiorequency catheter ablation in patients with paroxysmal non-valvular atrial fibrillation[J].Journal of Tianjin Medical University,2016,22(06):104.
[7]刘超,韩雅婷,杨 冰,等.基础医学模式生物酿酒酵母接合型的鉴定—PCR法[J].天津医科大学学报,2016,22(03):256.
　LIU Chao,HAN Ya-ting,YANG Bing,et al.Identification of the mating-type of model organism S. cerevisiae in basic medical science by PCR[J].Journal of Tianjin Medical University,2016,22(06):256.
[8]李立楠,李杉杉,刘 悦,等.神经酰胺诱导LX-2细胞凋亡抑制肝纤维发生的作用[J].天津医科大学学报,2016,22(04):283.
　LI Li-nan,LI Shan-shan,LIU Yue,et al.Effects of ceramide on promoted LX-2 cell apoptosis and inhibitting hepatic fibrosis[J].Journal of Tianjin Medical University,2016,22(06):283.
[9]王 锐,高靖达,张 卫,等.索利那新联合三联药物膀胱灌注治疗间质性膀胱炎/膀胱疼痛综合征疗效分析[J].天津医科大学学报,2016,22(04):341.
[10]陈妍,邢正英,李继彬,等.HPLC法测定黄丹兰颗粒剂中苦参碱和氧化苦参碱含量[J].天津医科大学学报,2016,22(05):452.

备注/Memo

备注/Memo:

作者简介 李冰（1983-），女，医师，硕士，研究方向：儿内科学；通信作者：冯凭，E-mail：duaiyong@sina.cn。

?

常用功能

更新日期/Last Update: 2015-11-27