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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

21卷

期数:

2015年04期

页码:

287-290

栏目:

基础医学

出版日期:

2015-07-15

文章信息/Info

Title:

Expressions of RASSF6 in gastric cancer and its association with clinicopathologic feature

文章编号:

1006-8147（2015）04-0287-04

作者:

刘新兰¹ ;  李淑璟² ;  张海霞²

  (1.宁夏医科大学总医院肿瘤医院肿瘤内科,银川750004;2.宁夏医科大学研究生院， 银川 750004)

Author(s):

LIU Xin -lan¹ ;  LI Shu -jing² ;  ZHANG Hai -xia²

 

( 1.Department of Oncology, Cancer Hospital,General Hospital of Ningxia Medical University, Yinchuan 750004,China; 2. Graduate School, Ningxia Medical University, Yinchuan 750004,China)

关键词:

胃癌; RASSF6;  临床病理特征

Keywords:

gastric cancer;  RASSF6 ;  ;  clinicopathologic feature

分类号:

R735.2

DOI:

-

文献标志码:

A

摘要:

 目的 ：探讨候选抑癌基因RASSF6在胃癌组织中的表达及其与临床病理特征的关系。方法 ：采用组织微阵列技术，通过免疫组化SP法检测100例胃癌组织和癌旁正常组织中RASSF6的表达，实时荧光定量RT-PCR和Western blot法检测20例新鲜胃癌组织和癌旁正常组织中RASSF6mRNA及蛋白的表达情况。结果: （1）胃癌组织中RASSF6阳性表达率明显低于癌旁正常组织（ 31.0% vs 76.0%，P＜0.05），胃癌组织中RASSF6mRNA和蛋白表达水平明显低于癌旁正常组织（3.949±0.816 vs 4.522±0.685和1.589±2.237 vs 5.723±1.939 ，P均＜0.05）。（2）RASSF6在胃癌组织中的表达与胃癌分化程度、浸润深度、淋巴结转移及胃癌分期差异均有统计学意义（P均<0.05）。结论:　RASSF6可能是胃癌中新的抑癌基因，其表达缺失与胃癌的发生和进展有关。

Abstract:

 Objective： To detect the expression of RASSF6 in gastric cancer, and to explore its association with clinicopathologic feature. Methods：Using tissue microarray technology, the expressions of RASSF6 in the gastric cancers and the adjacent normal tissue were analyzed by immunohistochemistry(n=100). Using real-time fluorescence quantitative PCR and Western blot ，the expressions of RASSF6 mRNA and protein levels in the gastric cancers and the adjacent normal tissues were analyzed(n=20) . Results：(1) RASSF6 was decreased in gastric cancer tissues as compared to the adjacent normal tissues(31.0% vs 76.0%，p<0.05). RASSF6 mRNA and protein were decreased in gastric cancer tissues as compared to the adjacent normal tissues (3.949±0.816 vs 4.522±0.685 and 1.589±2.237 vs 5.723±1.939,all of p<0.05). (2)The expression of RASSF6 was associated with the differentiation degree, infiltration depth, lymph node metastasis and gastric cancer points , and significant differences were found (p<0.05). Conclusion： RASSF6 may be a new tumor suppressor gene in gastric cancer tissue. The loss expression of RASSF6 is associated with occurrence and development of gastric cancer.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目 宁夏自然科学基金项目资助（NZ11271）

作者简介 刘新兰（1968-），女，主任医师，教授，硕士，研究方向：实体瘤的化疗和基础研究; E-mail:liuxinlan@csco.org.com。

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更新日期/Last Update: 2015-07-16