|本期目录/Table of Contents|

[1]王鹏燕,崔杉杉,常 红,等.氧化低密度脂蛋白对体外血管内皮细胞损伤的剂量反应关系[J].天津医科大学学报,2015,21(02):105-108.
　WANG Peng-yan,CUI Shan-shan,CHANG Hong,et al.Dose-response relationship of Oxidized low density lipoprotein on human umbilical vein vascular endothelial cells damage in vitro [J].Journal of Tianjin Medical University,2015,21(02):105-108.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
21卷
期数:
2015年02期
页码:
105-108
栏目:
基础医学
出版日期:
2015-03-20

文章信息/Info

Title:
Dose-response relationship of Oxidized low density lipoprotein on human umbilical vein vascular endothelial cells damage in vitro 
文章编号:
1006-8147（2015）02-0105-04
作者:
王鹏燕1; 崔杉杉1; 常 红1; 2; 李 文1; 黄国伟1; 高玉霞3
(天津医科大学公共卫生学院营养与食品卫生学系,天津 300070;2. 天津医科大学康复与运动医学系,天津 300070;3.天津医科大学总医院心内科,天津 300052)
Author(s):
WANG Peng-yan1;  CUI Shan-shan1;  CHANG Hong1; 2;  LI Wen1;  HUANG Guo-wei1;  Gao Yu-xia3
(1.Department of Nutrition and Food Science, School of Pubic Health, Tianjin Medical University, Tianjin 300070, China; 2.Department of Rehabilitation and Sports Medicine, Tianjin Medical University, Tianjin 300070, China; 3.Department of Cardiology, General Hospital, Tianjin Medical University, Tianjin 300052, China)
关键词:

氧化低密度脂蛋白; 血管内皮细胞; 氧化损伤

Keywords:
oxidized low density lipoprotein;  human umbilical vein endothelial cells;  oxidized injury
分类号:
R543.5
DOI:
-
文献标志码:
A
摘要:

目的：研究氧化低密度脂蛋白(ox-LDL) 对体外培养的人脐静脉血管内皮细胞(HUVEC)造成的损伤效应。方法：分别用不同浓度的ox-LDL作用于对数生长期的HUVEC细胞24 h，进行细胞形态学观察比较，采用四唑盐(MTT)比色法测定细胞活力，计算细胞生长抑制率，并测定细胞中丙二醛(MDA)含量。结果：随着ox-LDL浓度的增加，细胞形态发生明显变化，变得结构松散，ox-LDL高浓度时甚至出现细胞破碎、死亡。MTT实验的吸光度值(OD)在ox-LDL浓度为50、100、200 ?g/mL时，与对照组相比差异均具有统计学意义（P<0.05）。不同浓度干预24 h后细胞中 MDA含量与MTT实验结果一致。当ox-LDL浓度为50、100、200 ?g/mL时，细胞抑制率分别达到23.2%、45.2%、62.0%。结论：当HUVEC细胞暴露于ox-LDL 24 h后，干预浓度越大细胞活力越低，细胞生长抑制率越大，细胞中MDA含量增加。ox-LDL浓度为200 ?g/mL以内时，ox-LDL对HUVEC细胞可造成较明显的氧化损伤且呈现剂量效应关系。

Abstract:
Objective: To explore the dose-response relationship of oxidized low density lipoprotein (ox-LDL) on human umbilical vein vascular endothelial cells (HUVEC) damage in vitro. Methods: HUVEC were treated with different concentrations of ox-LDL for 24 h. Morphological?change?was observed. And then cell viability was measured by MTT and growth inhibition ratios were calculated. The content of MDA in endothelium cells was measured. Results: With the increase of the concentration of ox-LDL, cells morphology of HUVEC was deteriorated. Cells became loose, broken and eventually dead. When cells were exposed to different doses of ox-LDL for 24 h, compared with control group, cell viabilities of 50, 100 and 200 ?g/mL groups were decreased respectively and the differences were statistically significant (P<0.05). The statistical result of MDA concentration in cells after 24 h was consistent with MTT. The growth inhibition ratios for 50, 100, 200 ?g/mL group were 23.2%, 45.2%, 62.0%. Conclusion: When HUVEC are exposed to ox-LDL for 24 h, the cell viability decreases with the rise of the ox-LDL concentration while the cell growth inhibition rate and MDA content in the cells increase. When the concentration of ox-LDL is below 200 ug/mL, the HUVEC suffer marked oxidation damage and present dose effect relationship.

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金资助项目（81373002）
作者简介 王鹏燕（1989-），女，硕士在读，研究方向：营养与疾病；通信作者：高玉霞，E-mai: gaoyuxia@medmail.com.cn。
更新日期/Last Update: 2015-03-24