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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

20卷

期数:

2014年03期

页码:

220-223

栏目:

临床医学

出版日期:

2014-05-20

文章信息/Info

Title:

Research on the preparation of silver nanoparticles and its antibacterial property on Streptococcus mutans

文章编号:

1006-8147(2014)03-0220-04

作者:

李长娥; 于丹妮; 韩育植; 李 娜

(天津医科大学第二医院口腔科,天津300211)

Author(s):

LI Chang- e;  YU Dan-ni;  HAN Yu-zhi;  LI Na

（Department of Stomatology, The Second Hospital ,Tianjin Medical University, Tianjin 300211, China）

关键词:

葡萄糖; 纳米银; 变异链球菌; 抗菌性

Keywords:

glucose; silver nanoparticles; Streptococcus mutans; antibacterial ;  property

分类号:

-

DOI:

-

文献标志码:

A

摘要:

目的：对纳米银的制备及其对变异链球菌标准株和耐氟株的抗菌性进行研究。方法：采用葡萄糖还原硝酸银制备纳米银；采用纸片扩散法和微稀释法测定该纳米银对标准株及耐氟株的抗菌效果。结果：制备的纳米银为分散性好、均粒径约为13.04 nm的类球形粒子，它对标准株及耐氟株的抑菌环直径分别为（10.87±0.67）mm、（9.68±0.86）mm，MIC为（2.08±0.90）?g/mL、（2.60±0.90） ?g/mL，MBC 为（4.16±1.81）?g/mL、（5.21±1.81）?g/mL。该纳米银对标准株和耐氟株均有抗菌性（P <0.05），且对两菌株抗菌性的差异无统计学意义（P >0.05）。结论：葡萄糖还原硝酸银可制备出对变异链球菌标准株及耐氟株有较好抗菌性的纳米银。

Abstract:

Objective：To explore the preparation of silver nanoparticles and its antibacterial property on Streptococcus mutans. Methods：Silver nanoparticles were prepared by reducing silver nitrate with glucose. Disk diffusion method and microdilution method were used to explore the antimicrobial properties of the silver nanoparticles on standard strain and fluoride–resistant strain of Streptococcus mutans. Results：The silver nanoparticles were spherical with 13.04 nm mean size and very well dispersed. The mean diameters of inhibition zone of the nanoparticles on test bacteria were(10.87±0.67) mm, (9.68±0.86) mm respectively while minimum inhibitory concentration were (2.08±0.90) ?g/mL, (2.60±0.90) ?g/mL and minimum bactericidal concentration were (4.16±1.81) ?g/mL, (5.21±1.81) ?g/mL. Silver nanoparticles had excellent antibacterial effect on the test bacteria, showing significant statistical differences（P ＜0.05）. While the antibacterial property of silver nanoparticles on standard strain and fluoride–resistant strain revealed no significant difference（P ＞0.05）. Conclusion：Glucose -reduced silver nitrate can be used prepare silver nanoparticles which has marked antibacterial on standard strain and fluoride–resistant strain of Streptococcus mutans.

参考文献/References:

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相似文献/References:

[1]史梦柔,娄建石,李光远,等.正交法制备羧甲基壳聚糖－纳米银纳米微球[J].天津医科大学学报,2018,24(06):496.
　SHI Meng-rou,LOU Jian-shi,LI Guang-yuan,et al.Preparation of carboxymethyl chitosan-nano sliver nanoparticles by orthogonal design[J].Journal of Tianjin Medical University,2018,24(03):496.

备注/Memo

备注/Memo:

作者简介 李长娥（1987-），女，硕士在读，研究方向：口腔内科学；通信作者：于丹妮，E-mail:danni2468@eyou.com。

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更新日期/Last Update: 2014-05-12