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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

19卷

期数:

2013年06期

页码:

452-455

栏目:

临床医学

出版日期:

2013-11-20

文章信息/Info

Title:

Regulation of IL-8 and RANKL gene expression by lipopolysaccharide in human periodontal ligament fibroblasts

作者:

李新月

天津市口腔医院牙周病科，天津300041

Author(s):

LI Xin-yue

Ｄｅpartment of Periodontology, Tianjin Stomatological Hospital, Tianjin 300041, Chiｎａ

关键词:

牙龈卟啉单胞菌; 脂多糖; 牙周膜成纤维细胞; 白细胞介素8; NF-κB 受体活化因子配体

Keywords:

Porphyromonas gingivalis;  lipopolysaccharide;  human periodontal ligament fibroblasts;  interleukin 8;  receptor activator of NF-κB ligand

分类号:

R781.4

DOI:

-

文献标志码:

A

摘要:

目的：研究牙龈卟啉单胞菌（P. g.）脂多糖（LPS）对人牙周膜成纤维细胞（hPDLFs）中白细胞介素８（ＩＬ－８）和NF-κB 受体活化因子配体 (RANKL)基因表达的调节。 方法：取第4代hPDLFs细胞在含有10%胎牛血清（ＦＣＳ）的DMEM培养基中培养至完全融合后，分别用0.01、0.1、1、10 、100 mg/L P.g. LPS 作用hPDLFs 10 h，实时荧光定量PCR检测IL-8和RANKL mRNA的表达水平。 结果：P.g. LPS 浓度依赖性地刺激hPDLFs中IL-8 和RANKL mRNA的表达水平升高，10 mg/L P.g. LPS上调IL-8 mRNA水平，达到峰值（P＜0.01）；100 mg/L P.g. LPS显著增强RANKL mRNA水平（P＜0.01）。结论：高浓度的 P.g. LPS明显刺激hPDLFs中IL-8和RANKL的基因表达增强。

Abstract:

Objective: To study the regulation of gene expression of interleukin 8 (IL-8) and receptor activator of NF-κB ligand (RANKL) by Porphyromonas gingivalis lipopolysaccharide (P.g.LPS) in human periodontal ligament fibroblasts (hPDLFs). Methods: After the 4th generation hPDLFs cells were cultured in DMEM containing 10% fetal calf serum (FCS) to full fusion and hPDLFs were treated by 0.01,0.1,1,10, 100 mg/L P.g. LPS was applied for 10 h and real-time PCR was performed to measure IL-8 and RANKL mRNA expression levels. Results: P.g. LPS dose-dependent stimulated IL-8 and RANKL mRNA expression in hPDLFs, 10 mg/L P.g. LPS increased IL-8 mRNA levels to peak (P<0.01); RANKL mRNA levels were increased by 100 mg/L P.g. The result was statistically significantly (P<0.01). Conclusion: High concentration of P.g. LPS can stimulate IL-8 and RANKL gene expression increases significantly in hPDLFs.

参考文献/References:

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[1１］ Ｗａng Q P, Yang L, Manokawinchoke J, et al. Effects of 17β-estradiol on adiponectin regulation of the expression of osteoprotegerin and receptor activator of nuclear factor-κB ligand [J]. Bone, 2012, 51(3):515
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[2]薛 栋,蒋少云*,邓嘉胤,等.牙龈卟啉单胞菌脂多糖对人牙周膜细胞成骨分化能力的影响[J].天津医科大学学报,2015,21(04):299.
　XUE Dong,JIANG Shao-yun,DENG Jia-yin,et al.Effects of Porphyromonas gingivalis lipopolysaccharide on osteogenic differentiation of human periodontal ligament cells[J].Journal of Tianjin Medical University,2015,21(06):299.

备注/Memo

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更新日期/Last Update: 2013-12-20