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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

19卷

期数:

2013年06期

页码:

445-448

栏目:

基础医学

出版日期:

2013-11-20

文章信息/Info

Title:

Protective effects of hydrogen gas on acute lung injury induced by smoke inhalation in rats and its dose-effect relationship

作者:

刘 琪¹; 王大为¹; 陈 兴²; 刘 群²

1.天津医科大学研究生院，天津 300070；2.天津市第四医院烧伤科，天津 300222

Author(s):

LIU Qi¹;  WANG Da-wei¹;  CHEN Xing²;  LIU Qun²

１． Ｇｒａduate School, Tianjin Medical University, Tianjin 300070, China; 2. Department of Burns, The Fourth Hospital of Tianjin, Tianjin 300222, China

关键词:

吸入性损伤; 急性肺损伤; 氢气饱和生理盐水; 量效关系; 大鼠

Keywords:

inhalation injury;  acute lung injury;  hydrogen-rich saline;  dose-effect relationship;  rat

分类号:

R644

DOI:

-

文献标志码:

A

摘要:

目的：探讨不同剂量氢气饱和生理盐水（HS）对烟雾吸入性损伤大鼠急性肺损伤的保护作用。方法：30只SD大鼠随机分为5组：对照组（C）、吸入性损伤组（I）、氢气饱和生理盐水低剂量组（2.5 mL/kg，HSL）、中剂量组（5 mL/kg，HSM）、高剂量组（10 mL/kg，HSH），24 h后留取标本，透射电镜观察肺组织病理变化，检测血清中肿瘤坏死因子α（TNF-α），肺组织匀浆的丙二醛（MDA）含量，免疫组织化学法测定肺组织核因子-κBp65（NF-κBp65）的表达，TUNEL检测肺组织凋亡情况。结果：中、高剂量氢气饱和生理盐水能明显减轻吸入性损伤大鼠的急性肺损伤，降低炎性因子（TNF-α）的释放，减轻组织氧化损伤（MDA低于致伤组），抑制肺组织NF-κBp65的活化及细胞凋亡，且HSH优于HSM，HSM、HSH组间差异有统计学意义（P<0.05），而HSL组效果不明显。结论：氢气饱和生理盐水可减轻吸入性损伤大鼠的急性肺损伤，且存在一定的量效关系。

Abstract:

Objective: To discuss the therapeutical effect on smoke inhalation injury in rats under different doses of hydrogen-rich saline. Methods: Thirty rats were randomly divided into 5 groups: control group (C), inhalation group (I), low dose group (2.5 mL/kg, HSL), medium dose group (5 mL/kg, HSM), and high dose group (10 mL/kg, HSH). At 24 hours after injured, all rats were sacrificed and specimens were taken. The histopathological changes of lung tissue were observed using electron micrography. Tumor necrosis factor-α(TNF-α) in plasma was quantified, malondialdehyde (MDA) content of lung tissue was also detected, the NF-κBp65 in lung speciments was examined by immunohistochemistry technology, and the apoptosis of lung was monitored by terminal deoxynucleotidyl transfercase dUTP nik end labeling (TUNEL) assay. Results: When a comparison was made with the inhalation group, hydrogen-rich saline treatment could significantly relieve acute lung injury induced by inhalation injury as indicated by ultrastructural histopathologic changes under electron micrography and decrease TNF-α level. Furthermore, hydrogen-rich saline could inhibit lipid oxidation (MDA level was significantly decreased), the expression of NF-κBp65 and apoptosis of lung tissue, especially in medium and high dose group, while this change was not significant in low dose group. Conclusion: Hydrogen-rich saline treatment could significantly relieve acute lung injury induced by inhalation injury, with dose-effect relationship.

参考文献/References:

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备注/Memo

备注/Memo:

文章编号 １００６－８１４７（２０13）０6-0445-04 基金项目 天津市卫生局科技基金资助项目（2012KY05） 作者简介 刘琪（1986-），女，硕士在读，研究方向：吸入性损伤，重症烧伤，烧伤后整形；通信作者：刘群，E-mail：morningnewstar@ hotmail.com。

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更新日期/Last Update: 2013-12-20