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[1]曹楠,王倩,荆韧威,等.桑叶细胞外囊泡抑制酪氨酸酶活性及黑色素生成的研究[J].天津医科大学学报,2025,31(05):464-468.[doi:10.20135/j.issn.1006-8147.2025.05.0464]
　CAO Nan,WANG Qian,JING Renwei,et al.Study on the inhibition of tyrosinase activity and melanin production by extracellular vesicles derived from Morus alba[J].Journal of Tianjin Medical University,2025,31(05):464-468.[doi:10.20135/j.issn.1006-8147.2025.05.0464]
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桑叶细胞外囊泡抑制酪氨酸酶活性及黑色素生成的研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
31卷
期数:
2025年05期
页码:
464-468
栏目:
基础医学
出版日期:
2025-09-20

文章信息/Info

Title:
Study on the inhibition of tyrosinase activity and melanin production by extracellular vesicles derived from Morus alba
文章编号:
1006-8147(2025)05-0464-05
作者:
曹楠1; 王倩1; 荆韧威2; 尹海芳1
天津医科大学 1.医学技术学院；2.基础医学院，天津 300070
Author(s):
CAO Nan1; WANG Qian1;  JING Renwei2; YIN Haifang1
1.School of Medical Biotechnology； 2.School of Basic Medical Sciences，Tianjin Medical University， Tianjin 300070， China
关键词:
桑叶细胞外囊泡; 酪氨酸酶; 黑色素; 美白
Keywords:
extracellular vesicles derived from Morus alba;  tyrosinase;  melanin;  skin whitening
分类号:
R931.6
DOI:
10.20135/j.issn.1006-8147.2025.05.0464
文献标志码:
A
摘要:
目的：探讨桑叶细胞外囊泡（EVMA）对酪氨酸酶活性及黑色素生成的作用。方法：使用差速离心法从桑叶中提取EVMA；将实验分为正常对照组（NC组）、EVMA组和桑皮苷F组，荧光标记EVMA并与黑色素瘤细胞B16-F10细胞共培养，观察细胞对EVMA的摄取效率，并检测EVMA与酪氨酸酶的共定位程度，同时测定EVMA对B16-F10细胞酪氨酸酶活性的抑制效果，定量分析黑色素合成量；EVMA局部处理C57BL/6小鼠，观察小鼠皮肤毛囊色素沉积程度，评估其美白效果。结果：经分离纯化后可得粒径均一的EVMA，且在B16-F10细胞摄取实验中，与桑皮苷F组相比，EVMA组可更好地被B16-F10细胞摄取（t=103.3，P<0.001）；在B16-F10细胞的体外实验中，与桑皮苷F组相比，EVMA组能更精准地靶向酪氨酸酶区域并抑制其催化活性，从而使黑色素合成减少（t=7.796，P<0.05）；在动物模型中，EVMA抑制了黑色素的合成，使黑色素含量显著下调（t=4.641，P<0.05）。结论：EVMA兼具抑制酪氨酸酶活性与减少黑色素生成的双重功效。
Abstract:
Objective： To explore the effect of extracellular vesicles derived from Morus alba（EVMA） on tyrosinase activity and me-lanin production. Methods： EVMA were isolated from Morus alba via differential centrifugation. The experiments were divided into the negative control （NC） group， the EVMA-treated（EVMA） group， and the Mulberroside F group. Fluorescently labeled EVMA were cocultured with B16-F10 melanoma cells to assess cellular uptake efficiency， analyze co-localization with tyrosinase， determine the inhibitory effect on tyrosinase activity in B16-F10 cells， and quantify melanin synthesis levels. Additionally， topical application of EVMA on C57BL/6 mice was performed to evaluate the degree of follicular pigmentation in skin and assess whitening efficacy. Results： Following isolation and purification， EVMA exhibited uniform particle size distribution and demonstrated superior cellular uptake efficiency in B16-F10 melanoma cells uptake experiment compared to the Mulberroside F group（t=103.3，P<0.001）. In vitro experiments of B16-F10 cells further revealed that EVMA， in contrast to Mulberroside F， precisely targeted tyrosinase-containing regions and inhibited its catalytic activity， thereby significantly reducing melanin synthesis（t=7.796，P<0.05）. In vivo studies showed that EVMA treatment markedly suppressed melanin production， leading to significant downregulation of melanin content （t=4.641，P<0.05）. Conclusion： EVMA exhibits dual functionality in inhibiting tyrosinase activity and suppressing melanin production.

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备注/Memo

备注/Memo:
基金项目:双一流学科建设经费（116019-XK010301）
作者简介:曹楠（1999-），女，硕士在读，研究方向：植物细胞外囊泡相关研究；通信作者：尹海芳，E-mail：haifangyin@tmu.edu.cn。
更新日期/Last Update: 2025-10-01