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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

31卷

期数:

2025年05期

页码:

441-446

栏目:

基础医学

出版日期:

2025-09-20

文章信息/Info

Title:

Study on the regulation of lens epithelial cell biological behavior by IQGAP2 gene

文章编号:

1006-8147(2025)05-0441-06

作者:

刘明; 温凯; 梁景黎; 孙靖

天津医科大学眼科医院、眼视光学院、眼科研究所，国家眼耳鼻喉疾病临床医学研究中心天津市分中心，天津市视网膜功能与疾病重点实验室，天津300384

Author(s):

LIU Ming;  WEN Kai;  LIANG Jingli;  SUN Jing

Tianjin Key Laboratory of Retinal Functions and Diseases， Tianjin Branch of National Clinical Research Center for Ocular Disease， Eye Institute and School of Optometry， Tianjin Medical University Eye Hospital， Tianjin 300384， China

关键词:

细胞迁移; IQGAP2; 晶状体上皮细胞; 后发性白内障; 细胞增殖

Keywords:

cell migration;  IQGAP2;  lens epithelial cells;  posterior capsule opacification;  cell proliferation

分类号:

R77

DOI:

10.20135/j.issn.1006-8147.2025.05.0441

文献标志码:

A

摘要:

目的：探索IQGAP2基因对晶状体上皮细胞（LECs）生物学行为的影响。方法：选用人晶状体上皮细胞系（HLE-B3），采用小干扰RNA技术敲低HLE-B3中IQGAP2基因的表达。实验分2组：对照组（NC组）、Si-1组，经转染48 h后，检测HLE-B3中IQGAP2基因的mRNA和蛋白质表达水平。通过CCK-8实验和平板克隆实验等功能性实验，评估敲低IQGAP2基因后HLE-B3的增殖、迁移和侵袭等变化。结果：与NC组相比，Si-1组IQGAP2基因表达降低（t=6.437、10.05，均P<0.05），HLE-B3的增殖能力升高（t=3.321，P<0.01），细胞克隆数量以及迁移和侵袭细胞量增多（t=6.03、3.867、4.184，均P<0.01），而细胞黏附数量减少（t=3.316，P<0.01）。结论：敲低IQGAP2基因可促进HLE-B3的增殖、迁移、侵袭及克隆能力。

Abstract:

Objective： To investigate the effect of the IQGAP2 gene on the biological behavior of lens epithelial cells （LECs）. Methods： The human lens epithelial cell line（HLE-B3） was selected， and small interfering RNA （siRNA） technology was employed to knock down IQGAP2 gene expression in HLE-B3 cells. The experiment was divided into two groups： the NC group and the Si-1 group. After 48 hours of transfection， the mRNA and protein expression levels of the IQGAP2 gene in HLE-B3 cells were detected. Functional experiments， including the CCK-8 assay and plate colony formation assay， were conducted to evaluate changes in the proliferation， migration， and invasion of HLE-B3 cells following IQGAP2 gene knockdown. Results： Compared with the NC group， the Si-1 group exhibited significantly reduced IQGAP2 gene expression（t=6.437， 10.05， both P<0.05）， accompanied by increased pro-liferation capacity of HLE-B3 cells （t=3.321， P<0.01）， elevated the number of cell clones， migrating and invading cells （t=6.03， 3.867， 4.184， all P<0.01）. Conversely， the number of adherent cells decreased in the Si-1 group（t=3.316， P<0.01）. Conclusion： Knocking down the IQGAP2 gene can promote the proliferation， migration， invasion， and clonal ability of HLE-B3 cells.

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备注/Memo

备注/Memo:

基金项目:天津医科大学眼科医院高层次创新人才计划（YDYYRCXM-E2023-04）
作者简介:刘明（1999-），女，硕士在读，研究方向：眼科学；通信作者：孙靖，E-mail：iiitcs@tmu.edu.cn。

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更新日期/Last Update: 2025-10-01