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[1]张英,李豫峰,王婷,等.不同时期胚胎线粒体拷贝数与胚胎自身形态学分型的关系研究[J].天津医科大学学报,2023,29(01):55-58.
 ZHANG Ying,LI Yu-feng,WANG Ting,et al.Study on the relationship between the mitochondrial copy number of embryos at different stages and the morphological types of embryos[J].Journal of Tianjin Medical University,2023,29(01):55-58.
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不同时期胚胎线粒体拷贝数与胚胎自身形态学分型的关系研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
29卷
期数:
2023年01期
页码:
55-58
栏目:
临床医学
出版日期:
2023-01-20

文章信息/Info

Title:
Study on the relationship between the mitochondrial copy number of embryos at different stages and the morphological types of embryos
文章编号:
1006-8147(2023)01-0055-04
作者:
张英1李豫峰2王婷3赵华3
(1.河南省人民医院检验科,郑州450000;2.华中科技大学同济医学院附属同济医院生殖中心,武汉430000;3.河南省人民医院生殖医学中心,郑州450000)
Author(s):
ZHANG Ying1LI Yu-feng2WANG Ting3ZHAO Hua3
(1.Department of Clinical Laboratory,Henan Provincial People′s Hospital,Zhengzhou 450000,China;2.Reproductive Center,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430000,China;3.Reproductive Medicine Center,Henan Provincial People′s Hospital,Zhengzhou 450000,China )
关键词:
关键词 体外受精-胚胎移植胚胎线粒体拷贝数囊胚
Keywords:
in vitro fertilization-embryo transferembryomitochondriacopy number blastocyst
分类号:
R781.4
DOI:
-
文献标志码:
A
摘要:
目的:探讨人类不同形态学分型的卵裂期胚胎和囊胚与自身线粒体拷贝数的关系。方法:选择在生殖中心因女性双侧输卵管梗阻行体外受精-胚胎移植(IVF-ET)助孕顺利娩出健康胎儿后,自愿丢弃冷冻的卵裂期胚胎和囊胚用于科学研究。将冷冻的胚胎解冻复苏后,依据胚胎发育的时期,将所有胚胎分为卵裂期胚胎冷冻组(D3冷冻胚胎组,n=63)和囊胚冷冻组(D5冷冻胚胎,n=82),再根据冷冻胚胎的形态学评分标准,将冷冻卵裂期胚胎分为优质胚胎组(n=38)和较差胚胎组(n=25),将冷冻囊胚分为优质囊胚组(n=52)和较差囊胚组(n=30)。采用实时PCR检测胚胎线粒体拷贝数,比较不同阶段胚胎和相同阶段不同质量的胚胎与自身线粒体拷贝数的差异。结果:D3冷冻胚胎组线粒体拷贝数明显低于D5冷冻胚胎组(t=9.34,P<0.02),D3冷冻胚组中优质胚胎mtDNA拷贝数高于质量较差胚胎组(t=6.62,P<0.01),D5冷冻胚组中优质胚胎mt DNA拷贝数明显高于质量较差胚胎组(t=8.03,P<0.04)。结论:不同时期胚胎mt DNA拷贝数对胚胎的发育潜能有重要影响,mtDNA拷贝数越高,胚胎质量越好。
Abstract:
Objective: To study the relationship between mitochondrial copy number and embryos of different forms and types in different periods of human beings. Methods:Frozencleavage stage embryos and blastocysts voluntarily discarded for scientific research after successful pregnancy assisted by in vitro fertilization -embryo transfer (IVF-ET)in the reproductive center due to female bilateral fallopian tube obstructionwere selected. After thawing and recovery of frozen embryos,according to the different embryonic development period,all the embryos points D3 frozen embryos (cleavage stage embryo,n=63) and D5 frozen embryos group (blastocysts,n=82),and then based on the criteria of frozen embryos,frozen cleavage stage embryos can be divided into high quality embryos(n=38) and poor embryo group(n=25),frozen blastula were divided into poor quality blastocyst groups(n=52) and blastocyst groups (n=30). Real-time PCR was used to detect the mitochondrial copy number of single embryo,and the differences of mitochondrial copy number between embryos at different stages and embryos of different quality at the same stage and their own mitochondria were compared. Results:D3 frozen embryos group of mt DNA copy number was lower than that in group D5 frozen embryos,the difference was statistically significant(t=9.34,P< 0.02),D3 group of frozen embryo quality embryos mtDNA copy number higher than that of poor quality embryos group,difference was statistically significant(t=6.62,P< 0.01),D5 group of frozen embryo quality embryos mtDNA copy number significantly higher than the poor quality embryos group,difference was statistically significant(t=8.03,P< 0.04). Conclusion:The mtDNA copy number of embryos at different stages has an important effect on embryo development potential,and the higher the mtDNA copy number,the better the embryo quality.

参考文献/References:

[1] SCHATTEN H,SUN Q Y,PRATHER R. The impact of mitochondrial function/dysfunction on IVF and new treatment possibilities for infertility[J]. Reprod Biol Endocrinol,2014,12:111.
[2] TAKAHASHI T,IGARASHI H,AMITA M,et al. Molecular mechanism of poor embryo development in postovulatory aged oocytes:mini review[J]. J Obstet Gynaecol Res,2013,39(10):1431-1439.
[3] VAN BLERKOM J,DAVIS P. Mitochondrial signaling and fertilization[J].Mol Hum Reprod,2007,13(11):759- 770.
[4] SMEETS H J. Preventing the transmission of mitochondrial DNA disorders:selecting the good guys or kicking out the bad guys[J].Reprod Biomed Online,2013,27:599-610.
[5] SUTOVSKY P,MORENO RD,RAMALHO-SANTOS J,et al. Ubiquitin tag for sperm mitochondria[J]. Nature,1999,402(6760):371-372.
[6] VAN BLERKOM J,COX H,DAVISP.Regulatory roles for mitochondria in the peri-implantation mouse blastocytes:possible origins and developmental significance of differential DeltaPsim[J]. Reproduction,2006,131(5):961-976.
[7] ALPHA SCIENTISTS IN REPRODUCTIVE MEDICINE AND ESHRE SPECIAL INTEREST GROUP OF EMBRYOLOGY. The Istanbul consensus workshop on embryo assessment:proceedings of an expert meeting[J]. Hum Reprod,2011,26(6):1270-1283.
[8] SANTOS T A,EL SHOURBAGY S,ST JOHN J C.Mitochondrial content reflects oocyte variability and fertilization outcome[J]. Fertil Steril,2006,85 (3):584-591.
[9] KUJJO L L,PEREZ G I. Ceramide and mitochondrial function in aging oocytes:joggling a new hypothesis and old players[J]. 2012, 143(1):1-10.
[10] VAN BLERKOM J. Mitochondrial function in the human oocyte and embryo and their role in developmental competence[J]. Mitochondrion,2011,11(5):797-813.
[11] JANSEN R P,BURTON G J.Mitochondrial dysfunction in reproduction [J]. Mitochondrion,2004,4(5-6):577-600.
[12] CHIARATTI M R,MEIRELLES F V. Mitochondrial DNA copy number,a marker of viability for oocytes[J]. Biol Reprod,2010,83( 1) :1-2.
[13] CHAN C C,LIU V W,LAU E Y,et al.Mitochondrial DNA content and 4977 bp deletion in unfertilized oocytes[J]. Mol Hum Reprod,2005,11( 12) :843-846.
[14] MAY-PANLOUP P,CHR?魪TIEN M F,JACQUES C,et al. Low oocyte mitochondrial DNA content in ovarian insufficiency[J]. Hum Reprod,2005,20(3):593-597.
[15] SANTOS T A,EL SHOURBAGY S,ST JOHN J C. Mitochondrial content reflects oocyte variability and fertilization outcome[J]. Fertil Steril,2006,85(3):584-591.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金项目(81701444);河南卫健委科技创新杰出人才培养项目(YXKC2020021);河南省医学科技攻关省部共建重点项目(SBGJ202102016)
作者简介: 张英(1970-),女,主管技师,学士,研究方向:PCR检测;通信作者:赵华,E-mail:417692121@qq.com 。
更新日期/Last Update: 2023-02-01