|本期目录/Table of Contents|

[1]辛小敏,王秀敏,黄宏春.miRNA-370沉默对酒精性肝病大鼠的保护作用及机制研究[J].天津医科大学学报,2021,27(02):108-111.
 XIN Xiao-min,WANG Xiu-min,HUANG Hong-chun.Study on the protective effect and mechanism of microRNA-370 silencing on rats with alcoholic liver disease[J].Journal of Tianjin Medical University,2021,27(02):108-111.
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miRNA-370沉默对酒精性肝病大鼠的保护作用及机制研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
27
期数:
2021年02期
页码:
108-111
栏目:
基础医学
出版日期:
2021-03-15

文章信息/Info

Title:
Study on the protective effect and mechanism of microRNA-370 silencing on rats with alcoholic liver disease
文章编号:
1006-8147(2021)02-0108-04
作者:
辛小敏王秀敏 黄宏春
(安阳市人民医院消化内科,安阳 455000)
Author(s):
XIN Xiao-minWANG Xiu-minHUANG Hong-chun
(Department of Gastroenterology, Anyang People′s Hospital,Anyang 455000,China)
关键词:
酒精性肝病miRNA-370沉默信息调节因子6核因子E2相关因子2
Keywords:
alcoholic liver disease microRNA-370 silent information regulator 6 nuclear factor E2 related factor 2
分类号:
R575
DOI:
-
文献标志码:
A
摘要:
目的:探究微小RNA(miRNA)-370沉默对酒精性肝病(ALD)大鼠的作用及机制。方法:32只Sprague-Dawley(SD)大鼠随机分为4组:正常组(普通饲料)、模型组(40%酒精灌胃+隔日高脂饲料饲养)、空载组(40%酒精灌胃+隔日高脂饲料饲养+尾静脉注射含空载质粒的腺病毒)和沉默组(40%酒精灌胃+隔日高脂饲料饲养+尾静脉注射含si-miRNA-370质粒的腺病毒),每组各8只。用40%酒精灌胃联合隔日高脂饲料饲养法构建ALD大鼠模型。酶联免疫吸附法检测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)表达水平;实时荧光定量PCR法检测miRNA-370、沉默信息调节因子6(SIRT6)mRNA和Nrf2 mRNA表达水平;蛋白质免疫印迹法检测SIRT6和Nrf2表达水平。结果:与正常组比较,模型组SOD、GSH-Px、SIRT6 mRNA、Nrf2 mRNA、SIRT6和Nrf2水平降低,丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、甘油三酯(TG)、MDA和miRNA-370水平升高,差异均有统计学意义(q=10.306、14.839、9.040、10.645、5.836、8.775、11.098、10.066、8.569、4.976、12.435,均P<0.05)。与空载组比较,沉默组SOD、GSH-Px、SIRT6 mRNA、Nrf2 mRNA、SIRT6和Nrf2水平升高,ALT、AST、TG和miRNA-370水平降低,差异均有统计学意义(q=5.731、9.537、14.524、29.569、8.888、16.233、8.144、6.818、5.329、21.317,均P<0.05)。结论:MiRNA-370沉默可改善ALD大鼠肝脏损伤,升高SOD和GSH-Px水平,其机制可能与调控SIRT6和Nrf2表达有关。
Abstract:
Objective: To explore the effect and mechanism of microRNA(miRNA)-370 silencing on rats with alcoholic liver disease (ALD). Methods:Thirty-two Sprague-Dawley(SD)rats were randomly divided into 4 groups: normal group (common feed),model group (40% alcohol gavage + high-fat feed feeding every other day),no-load group(40% alcohol gavage + high-fat feed feeding every other day + tail vein injection of adenovirus containing empty plasmid) and silent group(40% alcohol gavage + high-fat feed feeding every other day + tail vein injection of adenovirus containing si-miRNA-370 plasmid),8 rats in each group. An ALD rat model was constructed using 40% alcohol gavage combined with high-fat feed every other day. Enzyme-linked immunosorbent assay was used to detect the expression levels of superoxide dismutase (SOD),glutathione peroxidase(GSH-Px) and malondialdehyde(MDA). Real-time fluorescence quantitative PCR method was used to detect miRNA-370 and SIRT6 mRNA and Nrf2 mRNA expression levels. Western blotting was used to detect the expression levels of SIRT6 and Nrf2. Results:Compared with the normal group,the levels of SOD,GSH-Px,SIRT6 mRNA,Nrf2 mRNA,SIRT6 and Nrf2 in the model group were decreased,and the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglyceride(TG),MDA and miRNA-370 were increased,the differences were statistically significant (q=10.306, 14.839,9.040,10.645,5.836,8.775,11.098,10.066,8.569,4.976,12.435,all P<0.05). Compared with the no-load group,the levels of SOD,GSH-Px,SIRT6 mRNA,Nrf2 mRNA,SIRT6,and Nrf2 in the silent group increased,while the levels of ALT,AST,TG and miRNA-370 decreased,the differences were statistically significant (q=5.731,9.537,14.524,29.569,8.888,16.233,8.144,6.818,5.329,

参考文献/References:

[1] 王烁,卿笃信. 白细胞介素-22在酒精性肝病中作用的研究进展[J].国际消化病杂志,2020,40(1):8
[2] Rehm J,Mathers C,Popova S,et al. Global burden of disease and injury and economic cost attributable to alcohol use and alcohol-use disorders[J]. Lancet, 2009,373(9682): 2223
[3] Li Y M,Fan J G,National Workshop on Fatty Liver and Alcoholic Liver Disease,et al. Guidelines of prevention and treatment for alcoholic liver disease (2018,China)[J]. J Dig Dis,2019,20(4):174
[4] 夏婷,张瑾,姚佳慧,等. 氧化应激在酒精性肝病中作用机制的研究进展[J].中国药理学通报,2017,33(10):1353
[5] Kim H G,Huang M,Xin Y,et al. The epigenetic regulator SIRT6 protects the liver from alcohol-induced tissue injury by reducing oxidative stress in mice[J]. J Hepatol,2019,71(5):960
[6] 邢会杰,宋琳亮,方梅霞,等. Nrf2在酒精性肝损伤模型中的研究进展[J].中国比较医学杂志,2014,24(4):74
[7] Ruan Z F,Xie M,Gui S J,et al. MiR-370 accelerated cerebral ischemia reperfusion injury via targeting SIRT6 and regulating Nrf2/ARE signal pathway[J]. Kaohsiung J Med Sci,2020,36(9):741
[8] 陈柚伶,魏嵋,汪静,等. 葛黄颗粒对酒精性肝病大鼠抗氧化应激及调节脂质代谢的作用机制[J]. 西南医科大学学报,2017,40(1):43
[9] Su Y,Li Q,Zheng Z,et al. Integrative bioinformatics analysis of miRNA and mRNA expression profiles and identification of associated miRNA-mRNA network in aortic dissection[J]. Medicine (Baltimore), 2019,98(24): e16013
[10] Ye G,Qin Y,Wang S,et al. Lamc1 promotes the Warburg effect in hepatocellular carcinoma cells by regulating PKM2 expression through AKT pathway[J]. Cancer Biol Ther,2019,20(5): 711
[11] 赵雪珂,穆茂,程明亮. 酒精性肝病与氧化应激[J].临床肝胆病杂志,2014,30(2):118
[12] 吴展,钟伏弟,袁观斗,等. 酒精性肝病与微小RNAs关系的研究进展[J].中华实验外科杂志,2020,37(1):194
[13] 周珂. MicroRNA-29b在酒精性肝病中的作用及机制研究[D].安徽医科大学,2019
[14] Brandon-Warner E,邵爽,王中峰. 肝星状细胞中的miR17-92基因簇可促进酒精性肝损伤的肝脏纤维化进程[J]. 临床肝胆病杂志,2016,32(9):1652
[15] 钱丽丽,闫秀明,张慧,等. miR-370靶向FoxO1抑制TNF-α诱导的肝癌细胞增殖、迁移和侵袭[J]. 中国老年学杂志,2020,40(9):1957
[16] Li X,Xu X,Chen K,et al. miR-370 Sensitizes TMZ response dependent of MGMT status in primary central nervous system lymphoma[J]. Pathol Oncol Res,2020,26(2):707
[17] 许思思,朱冬梅,李晓华. miR-370通过FoxM1相关通路调控人视网膜母细胞瘤细胞凋亡机制研究[J]. 眼科新进展,2020,40(3):221
[18] Zare M A,Zare A,Azarpira N,et al. The protective effect of bone marrow-derived mesenchymal stem cells in liver ischemia/reperfusion injury via down-regulation of miR-370[J]. Iran J Basic Med Sci,2019,22(6):683
[19] Zhaohui C,Shuihua W. Protective effects of SIRT6 against inflammation,oxidative stress,and cell apoptosis in spinal cord injury[J]. Inflammation,2020,43(5):1751
[20] 伏有为. 基于Nrf2/ARE通路探究黄精速溶粉对酒精性肝病小鼠的保护作用及可能机制[D]. 安徽医科大学,2019
[21] 刘建,聂广宁,李杨,等. MAPKs和Nrf2/ARE通路调控卵巢颗粒细胞氧化应激的机制研究[J]. 广东医学,2018,39(7):966

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备注/Memo

备注/Memo:
作者简介 辛小敏(1987-),女,主治医师,硕士,研究方向:消化内科;E-mail: y0a8i8@163.com。
更新日期/Last Update: 2021-03-10