|本期目录/Table of Contents|

[1]刘瑞琪,刘凡,贾智.维生素C对牙周膜干细胞中HDAC1和HDAC6表达的影响[J].天津医科大学学报,2021,27(02):105-107,146.
 LIU Rui-qi,LIU Fan,JIA Zhi.Vitamin C affects the expression of HDAC1 and HDAC6 in periodontal ligament stem cells[J].Journal of Tianjin Medical University,2021,27(02):105-107,146.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
27
期数:
2021年02期
页码:
105-107,146
栏目:
基础医学
出版日期:
2021-03-15

文章信息/Info

Title:
Vitamin C affects the expression of HDAC1 and HDAC6 in periodontal ligament stem cells
文章编号:
1006-8147(2021)02-0105-04
作者:
刘瑞琪刘凡贾智
(天津医科大学口腔医院综合科,天津 300070)
Author(s):
LIU Rui-qiLIU FanJIA Zhi
(Department of Endodontics,Stomatology Hospital, Tianjin Medical University,Tianjin 300070,China)
关键词:
组蛋白去乙酰化酶人牙周膜干细胞维生素CHDAC1HDAC6
Keywords:
histone deacetylasehuman periodontal ligament stem cellsvitamin CHDAC1HDAC6
分类号:
R781.4
DOI:
-
文献标志码:
A
摘要:
目的:探究维生素C对牙周膜干细胞组蛋白去乙酰化酶(HDAC)1和HDAC6表达的影响。方法:收取临床上因正畸需要拔除的无龋坏前磨牙,分离并培养人牙周膜干细胞。流式细胞仪测定牙周膜干细胞表面标志物。qPCR检测添加5、20、80、320 mmol/L维生素C后,人牙周膜干细胞中HDAC1和HDAC6 mRNA的表达情况。结果:维生素C促进HDAC1(F=45.76,P<0.01)、HDAC6(F=119, P<0.01)的表达,且随着浓度的增加,HDAC1、HDAC6表达显著增加。结论:维生素C促进牙周膜干细胞中HDAC1和HDAC6表达。
Abstract:
Objective: To reveal the effects of vitamin C on the expression of HDAC1 and HDAC6 mRNA in periodontal ligament stem cells. Methods: Human periodontal ligament stem cells were isolated and cultured from caries free premolars. Determination of surface markers of periodontal ligament stem cells by flow cytometry. qPCR was used to analyze the expression of HDAC1 and HDAC6 mRNA in human periodontal ligament stem cells treated with different concentrations of vitamin C(5,20,80,320 mmol/L). Results:Vitamin C promoted the expression of HDAC1 and HDAC6,and the expression of HDAC1(F=45.76,P<0.01) and HDAC6(F=119,P<0.01) increased significantly with the increase of vitamin C concentration. Conclusion: The results of the experiment indicate that vitamin C promotes the expression of HDAC1 and HDAC6 in periodontal ligament stem cells.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介 刘瑞琪(1994-),硕士在读,研究方向:口腔内科学及口腔细胞分子生物学;通信作者:贾智,E-mail: jiazhi1962@tmu.edu.cn。
更新日期/Last Update: 2021-03-10