|本期目录/Table of Contents|

[1]余胡燕,刘颖,顾秋华,等.健康成人尿源性干细胞的分离、培养与鉴定[J].天津医科大学学报,2021,27(01):1-5,21.
　YU Hu-yan,LIU Ying,GU Qiu-hua,et al.Isolation, cultivation and identification of healthy adults urine-derived stem cells[J].Journal of Tianjin Medical University,2021,27(01):1-5,21.
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健康成人尿源性干细胞的分离、培养与鉴定(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
27卷
期数:
2021年01期
页码:
1-5,21
栏目:
基础医学
出版日期:
2021-01-20

文章信息/Info

Title:
Isolation, cultivation and identification of healthy adults urine-derived stem cells
文章编号:
1006-8147（2021）01-0001-06
作者:
余胡燕; 刘颖; 顾秋华; 连宇航; 鲁振宇; 李栋
天津医科大学总医院肾脏内科，天津300052
Author(s):
YU Hu-yan; LIU Ying; GU Qiu-hua; LIAN Yu-hang; LU Zheng-yu; LI Dong
Department of Nephrology，General Hospital，Tianjin Medical University，Tianjin 300052，China
关键词:
尿源性干细胞; 体外培养; 分离; 鉴定
Keywords:
urine-derived stem cell; in vitro culture; isolation; identification
分类号:
R329.2
DOI:
-
文献标志码:
A
摘要:
目的：分离培养和鉴定健康成人尿源性干细胞（USCs）。方法：收集3名健康成人新鲜尿液，采用贴 壁培养法分离培养细胞。进一步观察细胞形态，对细胞进行染色体核型分析，流式细胞术检测细胞表面标志物 CD73、CD90、CD105、CD34和CD45的表达情况，采用茜素红及油红O染色分别检测钙结节和脂质的形成以验证细 胞成骨和成脂分化能力。结果：成功从健康成人新鲜尿液中分离到USCs，该细胞贴壁生长，其外观呈长梭形， 具有较强的体外增殖能力，且传代过程中其染色体核型并未改变；细胞表面标志物CD73、CD90、CD105的阳性 表达率均达到99%，CD34和CD45均呈阴性，阳性率均低于2%；茜素红和油红O染色均呈阳性。结论：从健康成人 尿液中成功分离培养出了USCs，并且鉴定了其具有较强的稳定增殖和分化能力。
Abstract:
Objective: To isolate，culture，and identify the urine-derived stem cells（USCs） from healthy adults. Methods: Three fresh urine samples from healthy adults were collected and cells were isolated and cultured by adherent culture. Further，we observed the cell morphology， analyzed the chromosome karyotype，detected the expression of cell surface markers CD73，CD90， CD105，CD34 and CD45 by flow cytometry. Alizarin red and oil red O staining were used to detect the formation of calcium nodules and lipid to verify the ability of osteogenic and adipogenic differentiation. Results: USCs were successfully obtained from the fresh urine of healthy adults. The cells adhered to the plastic，had a long fusiform appearance and a strong ability of proliferation in vitro，and had no change in chromosome karyotype during subculture. The positive expression rates of cell surface markers CD73，CD90 and CD105 were 99%. CD34 and CD45 are negative and positive rates were both less than 2%. Alizarin red and oil red O staining were positive. Conclusion: USCs are successfully isolated and cultured from the urine of healthy adults，and its strong ability of stable proliferation and differentiation is identified.

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备注/Memo

备注/Memo:
基金项目 赵以成医学科学基金青年孵育项目（ZYYFY2018002）
作者简介 余胡燕（1995-），女，硕士在读，研究方向：肾病学；通信作者：李栋，E- mail:lidong430@126.com。
更新日期/Last Update: 2021-01-10