|本期目录/Table of Contents|

[1]李璐,李晓飞,孙悦,等.结直肠癌患者肠道菌群促进Apcmin/+小鼠肠道腺瘤进展的研究[J].天津医科大学学报,2019,25(06):551-555.
 LI Lu,LI Xiao-fei,SUN Yue,et al.Gut microbiota from patients with colorectal cancer promotes the progression of intestinal adenoma in Apcmin/+ mice[J].Journal of Tianjin Medical University,2019,25(06):551-555.
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结直肠癌患者肠道菌群促进Apcmin/+小鼠肠道腺瘤进展的研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
25
期数:
2019年06期
页码:
551-555
栏目:
基础医学
出版日期:
2019-11-20

文章信息/Info

Title:
Gut microbiota from patients with colorectal cancer promotes the progression of intestinal adenoma in Apcmin/+ mice
文章编号:
1006-8147(2019)06-0551-05
作者:
李璐李晓飞孙悦刘天宇王邦茂曹海龙姜葵
(天津医科大学总医院消化科,天津 300052)
Author(s):
LI Lu LI Xiao-fei SUN Yue LIU Tian-yu WANG Bang-mao CAO Hai-long JIANG Kui
(Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, Tianjin 300052, China)
关键词:
结直肠癌粪菌移植Apcmin/+小鼠
Keywords:
colorectal cancerfecal microbiota transplantation Apcmin/+ mice
分类号:
R574.6+R730
DOI:
-
文献标志码:
A
摘要:
目的:探讨结直肠癌患者的肠道菌群对Apcmin/+小鼠肠道腺瘤进展的作用。方法:将4周龄雌性Apcmin/+小鼠随机分为PBS组(灌服无菌磷酸盐缓冲液,PBS)、FMT-H组(灌服健康人菌液)和FMT-C组(灌服结直肠癌患者菌液),每组10只。给予3d抗生素鸡尾酒预处理后,进行为期8周的粪菌移植。光学显微镜下观察并记录每只小鼠肠道腺瘤负荷。收集肠道组织进行免疫组化评价肿瘤细胞增殖水平及Wnt信号通路活化情况。结果:与FMT-H组相比,FMT-C组腺瘤数量和肠道肿瘤组织中Ki-67阳性细胞百分比显著增加。FMT-C组中Wnt信号通路关键蛋白β-catenin出现异位表达,下游的细胞周期蛋白(cyclinD1)表达增多。结论:结直肠癌患者的肠道菌群可诱导肠道慢性低度炎症、活化Wnt信号通路、促进Apcmin/+小鼠肠道腺瘤进展。
Abstract:
Objective: To investigate the efficacy of gut microbiota from patients with colorectal cancer(CRC) on the progression of intestinal adenomas in Apcmin/+ mice. Methods: The female Apcmin/+ mice aged 4 weeks were divided into three groups: PBS group (gavage of sterile phosphate buffered solution,PBS), FMT-H group (gavage of mixed fecal samples from healthy people) and FMT-C group (gavage of mixed fecal samples from patients with CRC),10 mice in each group. After receiving antibiotic cocktails in drinking water for three days, the mice were inoculated with a total of 16 times transfer experiments over the subsequent 8 weeks. The intestinal adenoma burden of each mouse was observed and recorded under an optical microscope. Intestinal tissue was collected for immunohistochemistry to evaluate tumor cell proliferation and Wnt signaling pathway activation. Results: Compared with FMT-H group , the number of adenomas and the percentage of ki-67 positive cells in intestinal tumor tissues in the FMT-C group significantly increased. Heterotopic expression of β-catenin and expression of downstream cyclin (cyclinD1) were increased in the FMT-C group. Conclusion: Gut microbiota of CRC patients promotes intestinal adenoma progression in Apcmin/+ mice by inducing chronic low-grade intestinal inflammation and activating Wnt signaling pathway.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金(81570478,81741075);天津市自然科学基金(17JCYBJC24900)
作者简介 李璐(1993-),女,硕士在读,研究方向:肠道微生态;通信作者: 姜葵,E-mail: kjiang@tmu.edu.cn。
更新日期/Last Update: 2020-01-20