|本期目录/Table of Contents|

[1]马晓霞,孙 旸,马 瑞,等.IL-6诱导卵巢癌细胞对他莫西芬产生耐药的实验研究[J].天津医科大学学报,2019,25(05):436-439.
 MA Xiao-xia,SUN Yang,MA Rui,et al.IL-6 induces ovarian cancer cell resistance to tamoxifen[J].Journal of Tianjin Medical University,2019,25(05):436-439.
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IL-6诱导卵巢癌细胞对他莫西芬产生耐药的实验研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
25
期数:
2019年05期
页码:
436-439
栏目:
基础医学
出版日期:
2019-09-20

文章信息/Info

Title:
IL-6 induces ovarian cancer cell resistance to tamoxifen
文章编号:
1006-8147(2019)05-0436-04
作者:
马晓霞12孙 旸3马 瑞2崔志坤2王 越2郭小芹2邓为民1
(1.天津医科大学免疫学系,天津300070;2.武警后勤学院病原生物学教研室,天津300309;3.武警特色医学中心妇产科,天津 300163)
Author(s):
MA Xiao-xia12 SUN Yang3 MA Rui2 CUI Zhi-kun2 WANG Yue2 GUO Xiao-qin2 DENG Wei-min1
(1.Department of Immunology, Tianjin Medical University, Tianjin 300070, China; 2. Logistics University of People’s Armed Police Force, Tianjin 300309, China; 3.Department of Gynecology and Obstetrics, Armed Police Characteristic Medical Center, Tianjin 300163, China)
关键词:
IL-6卵巢癌细胞基因稳定转染与克隆筛选抗雌激素治疗耐药
Keywords:
interleukin-6 ovarian cancer cells gene stable transfection and clonal screening anti-estrogen treatment resistance
分类号:
R737.31
DOI:
-
文献标志码:
A
摘要:
目的:研究IL-6在诱导卵巢癌细胞对他莫西芬(TAM)产生耐药中的作用。方法:选择A2780(不分泌IL-6,但表达其受体,且对TAM敏感)和CAOV3(高表达IL-6及其受体,且对TAM耐药)细胞作为研究模型,应用重组IL-6短期处理或将ss/as IL-6基因稳定转染至A2780(该数据已有文章发表)及CAOV3细胞,通过MTT、RT-PCR、ELISA等观察内源性及外源性IL-6是否影响卵巢癌细胞对TAM的敏感性。结果:经稳定转染得到IL-6中、高抑制表达的2个CAOV3/asIL-6细胞克隆,与空载体转染细胞CAOV3/pcDNA3.1+相比,IL-6分泌水平分别下降了63.82%和75.69%(P<0.05),其IL-6 mRNA水平与其分泌水平相似;外源性IL-6处理及内源性过表达IL-6均可诱导A2780 细胞对TAM产生耐药,而内源性抑制IL-6表达则可恢复CAOV3细胞对TAM的敏感性。结论:IL-6可诱导卵巢癌细胞对TAM产生耐药,IL-6有可能是卵巢癌抗雌激素治疗耐药中的重要一环,有望成为逆转卵巢癌抗雌激素治疗耐药的重要靶点。
Abstract:
Objective: To explore the effect of tamoxifen resistance caused by exogenous and endogenous IL-6 in ovarian cancer cells. Methods: A2780 and CAOV3 cells were used as research models; we retreated with exogenous IL-6 to obtain A2780/preIL-6 cells. The antisense IL-6 gene was stably transfected into CAOV3 cells, and the IL-6 mRNA and IL-6 secretion levels of stably transfected cells were detected by RT-PCR and ELISA. Ovarian cancer cell activity was measured by MTT assay. Results: CAOV3/asIL-6 with different IL-6 secretion levels were stably transfected, and their IL-6 secretion levels were decreased by 63.82% and 75.69%, respectively, compared with their untransfected cells CAOV3(P<0.05), and its IL-6 mRNA level was consistent with its secretion level;Exogenous IL-6, endogenous overexpression of IL-6 induced resistance to tamoxifen in A2780 cells, whereas inhibition of IL-6 expression restored the sensitivity of CAOV3 cells to tamoxifen. Conclusion: IL-6 can induce tamoxifen resistance in ovarian cancer cells. IL-6 may be an important part of anti-estrogen resistance in ovarian cancer, and it is expected to be an important factor in reversing the anti-estrogen resistance of ovarian cancer.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金资助项目(81502256,81572852);天津市自然科学基金重点项目 (18JCZDJC33200,18YFZCSY00040)
作者简介 马晓霞(1993-),女,硕士在读,研究方向:肿瘤免疫;通信作者:邓为民,E-mail:dengweimin@tmu.edu.cn;郭小芹,E-mail: guoxiaoqinlet@163.com。
更新日期/Last Update: 2019-10-11