|本期目录/Table of Contents|

[1]李俊强,靳美娜,李冰洁,等.HPLC-DAD法同时测定SHF抗抑郁皂苷部位中5个指标成分含量[J].天津医科大学学报,2017,23(05):464-468.
 LI Jun-qiang,JIN Mei-na,LI Bing-jie,et al.HPLC-DAD simultaneous determination of contents of 5 constituents in SHF saponin[J].Journal of Tianjin Medical University,2017,23(05):464-468.
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HPLC-DAD法同时测定SHF抗抑郁皂苷部位中5个指标成分含量(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
23
期数:
2017年05期
页码:
464-468
栏目:
技术与方法
出版日期:
2017-09-20

文章信息/Info

Title:
HPLC-DAD simultaneous determination of contents of 5 constituents in SHF saponin
文章编号:
1006-8147(2017)05-0464-05
作者:
李俊强靳美娜李冰洁王冉冉乔 卫
(天津医科大学药学院, 天津市临床药物关键技术重点实验室,天津 300070)
Author(s):
LI Jun-qiang JIN Mei-na LI Bing-jie WANG Ran-ran QIAO Wei
(College of Pharmacy, Tianjin Medical University,Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics,? Tianjin 300070, China)
关键词:
酸枣仁合欢方皂苷HPLC-DAD含量
Keywords:
suanzaorenhehuanfang saponin HPLC-DAD content
分类号:
R927.2
DOI:
-
文献标志码:
A
摘要:
目的:应用高效液相-二极管阵列法(HPLC-DAD)建立同时测定酸枣仁合欢方(SHF)抗抑郁皂苷部位中氧化芍药苷、芍药内酯苷、芍药苷、(-)-丁香树脂酚-4-O-β-D-呋喃芹糖基-(1→2)-β-D-吡喃葡萄糖苷、白桦酯酸等5个指标成分含量的分析方法,并指认5个指标成分的来源,以建立SHF抗抑郁有效部位的质量控制方法。方法:色谱柱:Agilent TC-C18柱(规格:4.6 mm × 250 mm,5 mm);流动相:乙腈(A)-0.1%磷酸水;梯度洗脱,运行时间:85 min,洗脱程序为:0~9 min,7%~14%A;9~20 min,14%A;20~31 min,14%~20%A;31~32 min,20%~23%A;32~44 min,23%~32%A;44~52 min,32%~36%A;52~65 min,36%~63%A; 65~85 min,63%~100%A。流速:1 mL×min-1;检测波长:204 nm;柱温:30 ℃;进样量:20 mL。通过比较各单味药材皂苷部位与缺单味药的阴性皂苷部位、混合标准品、全方皂苷部位的HPLC图,指认出5个指标成分的来源结果:在上述色谱条件下各成分峰均能达到分离,分离度良好。在各自的测定浓度范围内,SHF抗抑郁皂苷部位中5个指标成分均呈现良好的线性关系 (R2>0.999),其回收率均不小于97.0%。5个指标成分中氧化芍药苷、芍药内酯苷、芍药苷均来源于白芍;(-)-丁香树脂酚-4-O-β-D-呋喃芹糖基-(1→2)-β-D-吡喃葡萄糖苷全部来源于合欢皮;白桦酯酸在四味药材中均有体现。结论:HPLC-DAD测定方法快速、灵敏、重现性良好,实现了一次进样同时测定SHF抗抑郁皂苷部位中5种指标成分含量,为研制简单有效、质量可控的创新中药奠定了基础。
Abstract:
Objective: To establish an HPLC method for the simultaneous determination of oxypaeoniflora, albiflorin, paeoniflorin, (-)-syringaresnol-4-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside and betulinic acid in suanzaorenhehuanfang (SHF) Saponin with HPLC-DAD analysis and identify the sources of the 5 constituents. Furthermore, the quality control standard of SHF Saponin was established, which has anti-depression effect. Methods: HPLC-DAD analysis was performed on an Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm). The mobile phase: acetonitrile (A) -0.1% phosphoric acid aqueous solution. The elution mode was gradient elution: 0~9 min, 7%~14% A; 9~20 min, 14% A; 20~31 min, 14%~20% A; 31~32 min, 20%~23% A; 32~44 min, 23%~32% A; 44~52 min, 32% A~36%; 52~65 min, 36%~63% A; 65~85 min, 63%~100% A. The flow rate: 1 mL×min-1. The detection wavelength: 204 nm. The column temperature: 30 ℃. The injection volume: 20 μL. The sources of the 5 constituents were identified by comparing the HPLC chromatogram of the saponin of a single herb, the negative saponin lack of 1 herb, mixture reference substance and SHF Saponin. Results: In this condition, each peak was well separated and the 5 constituents had good linear relation with their determination of the concentration (R2>0.999). The recovery rate was not less than 97.0%. Among 5 constituents, oxypaeoniflora, albiflorin and paeoniflorin belonged to Radix Paeoniae Alba (dried root of Ranunculaceae peony Paeonia lactiflora Pall). (-)-Syringaresnol-4-O-β-D-apiofuranosyl-(1→2) -β- D-glucopyranoside belonged to Cortex Albiziae (dried stem bark of the leguminous plant Albizzia julibrissin Durazz). Besides, betulinic acid belonged to 4 herbs of SHF. Conclusion: The method is fast, sensitive and reproducible, could make simultaneous determination of 6 constituents of SHF Saponin possible. It also provides a theoretical basis and experimental basis for the development of effective, quality-controllable and innovative Traditional Chinese Medicine (TCM).

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金资助项目(81173530),天津市自然科学基金重点项目(12YFJZJC08100)

作者简介 李俊强(1990-),男,硕士在读,研究方向:中药分析及网络药理学;通信作者:乔卫, E-mail:qiaowei@tmu.edu.cn。

更新日期/Last Update: 2017-09-21