|本期目录/Table of Contents|

[1]樊保佑,林伟,史桂东,等.探究激活态雪旺细胞与生物可降解聚己内酯支架的生物相容性[J].天津医科大学学报,2017,23(05):398-402.
 FAN Bao-you,LIN Wei,SHI Gui-dong,et al.Study on the biocompatibility of PCL scaffold with activated Schwann cells[J].Journal of Tianjin Medical University,2017,23(05):398-402.
点击复制

探究激活态雪旺细胞与生物可降解聚己内酯支架的生物相容性(PDF)
分享到:

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
23
期数:
2017年05期
页码:
398-402
栏目:
基础医学
出版日期:
2017-09-20

文章信息/Info

Title:
Study on the biocompatibility of PCL scaffold with activated Schwann cells
文章编号:
1006-8147(2017)05-0398-05
作者:
樊保佑林伟史桂东任一鸣魏志坚郝岩周先虎冯世庆
(天津医科大学总医院骨科,天津 300052)

?

Author(s):
FAN Bao-you LIN Wei SHI Gui-dong REN Yi-ming WEI Zhi-jian HAO Yan ZHOU Xian-hu FENG Shi-qing
(Department of Orthopedics, General Hospital ,Tianjin Medical University,Tianjin, China, 300052)
关键词:
激活态雪旺细胞PCL支架生物相容性
Keywords:
activatedschwann cells polycaprolactone scaffoldbiocompatibility
分类号:
R68
DOI:
-
文献标志码:
A
摘要:
目的 :探究激活态雪旺细胞(ASC)与非激活态雪旺细胞(SC)与生物可降解聚己内酯(PCL)支架的生物相容性。方法:实验组由结扎7 d Wistar大鼠坐骨神经提取激活态雪旺细胞,对照组由未结扎坐骨神经提取非激活态雪旺细胞。将细胞分别种植在PCL支架上,通过CCK-8检测细胞增殖活性,通过结晶紫观察细胞分布,激光共聚焦显微镜观察细胞形态和功能表达。结果: ASC与SC相比S-100阳性率无明显差异;PCL支架上ASC增殖率明显高于SC;ASC能够贴附PCL纤维生长,可沿纤维长轴分布,进入支架内部,并且表达髓鞘碱性蛋白(MBP)。结论:激活态雪旺细胞比非激活态雪旺细胞能够更好地在PCL支架上生长,PCL支架具有良好的生物相容性。
Abstract:
Objective: To study the biocompatibility of polycaprolactone scaffold with activated Schwann cells(ASC)and normal Schwann cells(SC). Methods:There were two groups: ASC extracted from ligated sciatic nerves experiment group; SC extracted from normal sciatic nerves control group. Proliferation activity was tested through the CCK- 8 test, and neurotrophic factors was dectected by real-time PCR and the distribution and shape of cells was observed by crystal violet staining and confocal laser scanning microscope. Results: There was no difference between ASC and SC in the positive rate of S-100; the proliferation rate of ASC was significantly higher than that of SC in PCL; ASC could attach PCL fiber and move along the long axis of the fiber into the scaffolds, and express myelin basic protein (MBP). Conclusion:Activated Schwann cells can grow better in PCL scaffold than non-activated Schwann cells, and PCL scaffold has good biocompatibility.

参考文献/References:

[1] Armati P J, Mathey E K.An update on Schwann cell biology--immunomodulation, neural regulation and other surprises [J].J NeurolSci, 2013, 333(1/2):68

[2] Webber C, Zochodne D. The nerve regenerative microenvironment: early behavior and partnership of axons and Schwann cells [J]. ExpNeurol, 2010, 223(1):51

[3] Allodi I, Udina E, Navarro X. Specificity of peripheral nerve regeneration: interactions at the axon level [J]. ProgNeurobiol, 2012, 98(1):16

[4] 冯世庆. 雪旺细胞移植治疗脊髓损伤研究进展[J]. 中国现代神经疾病杂志, 2004, 4(5):276

[5] 冯世庆, 孔晓红, 孙振辉,等. 大鼠神经干细胞与小鼠雪旺细胞混合培养的研究[J]. 中华实验外科杂志, 2006, 23(1):81

[6] 周先虎, 郝岩, 冯世庆,等. 激活态雪旺细胞源性神经营养因子对脐血间充质干细胞分化的影响[J]. 中华实验外科杂志, 2012, 29(4):719

[7]NagarajanR,LeN, MahoneyH, et al. Deciphering peripheral nerve myelination by using Schwann cell expression profiling [J]. ProcNatlAcadSci U S A, 2002, 99(13):8998

[8] Arthur-Farraj P, Latouche M, Wilton D, et al. C-Jun reprograms Schwann cells of Injured nerves to generate a repair cell essential for regeneration[J]. Neuron, 2012, 75(4):633

[9] Barrette B, Calvo E, Vallières N, et al. Transcriptional profiling of the injured sciatic nerve of mice carrying the Wld(S) mutant gene: identification of genes involved in neuroprotection, neuroinflammation, and nerve regeneration [J]. Brain BehavImmun, 2010, 24(8):1254

[10] Middleton J C, Tipton A J. Synthetic biodegradable polymers as orthopedic devices [J]. Biomaterials, 2000, 21(21):2335

[11] Ng K W, Achuth H N, Moochhala S, et al. In vivo evaluation of an ultra-thin polycaprolactone film as a wound dressing [J]. J BiomaterSciPolym Ed, 2007, 18(7):925

[12] Woodruff M A, Hutmacher D W.The return of a forgotten polymer—Polycaprolactone in the 21st century [J].ProgpolymSci, 2010, 35(10):1217

[13] Puppi D, Chiellini F, Dash M, et al. Biodegradable Polymers for Biomedical Applications[M]// Optical Detection Theory for Laser Applications /. Wiley-Interscience, 2002:424

[14] Stoll G, Müller H W. Nerve injury, axonal degeneration and neural regeneration: basic insights [J]. Brain Pathol, 1999, 9(2):313

[15] Jessen K R, Mirsky R, Lloyd A C. Schwann cells: Development and role in nerve repair [J]. CshPerspectBiol, 2015, 7(7) :a020487

[16] Bolaina-Lorenzo E, Martinez-Ramos C, Monleon-Pradas M, et al. Electrospunpolycaprolactone/chitosan scaffolds for nerve tissue engineering: physicochemical characterization and Schwann cell biocompatibility[J]. Biomed Mater, 2016, 12(1): 015008

[17] Jang C H, Lee H, Kim M, et al. Effect of polycaprolactone/collagen/hUCS microfiber nerve conduit on facial nerve regeneration [J]. Int J BiolMacromol, 2016, 93(PtB):1575

[18] Sharifi F, Patel B B, Dzuilko A K, et al. Polycaprolactonemicrofibrous scaffolds to navigate neural stem cells [J]. Biomacromolecules, 2016, 17(10):3287

相似文献/References:

备注/Memo

备注/Memo:
基金项目国家自然科学基金重点项目基金资助(81330042);国家自然科学基金面上项目(81371957);国家科技部中俄合作专项项目(2014DFR31210);国家自然科学基金国际合作与交流项目(81620108018);天津市科委重点项目(14ZCZDSY00044,13RCGFSY19000)

作者简介樊保佑(1990-),男,硕士在读,研究方向:脊髓损伤;

通信作者:冯世庆, E-mail:sqfeng@tmu.edu.cn。

更新日期/Last Update: 2017-09-20