|本期目录/Table of Contents|

[1]刘 媛,袁 杰,张 洁,等.Ebp1的表达对乳腺癌细胞增殖和侵袭的影响[J].天津医科大学学报,2015,21(01):14-17.
 LIU Yuan,YUAN Jie,ZHANG Jie,et al.Influence of the expression of Ebp1 on the proliferation and invasion of breast cancer cells[J].Journal of Tianjin Medical University,2015,21(01):14-17.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
21
期数:
2015年01期
页码:
14-17
栏目:
基础医学
出版日期:
2015-01-20

文章信息/Info

Title:
Influence of the expression of Ebp1 on the proliferation and invasion of breast cancer cells
文章编号:
1006-8147(2015)0-0014-04
作者:
刘 媛袁 杰张 洁赵元元牛瑞芳
(天津医科大学肿瘤医院肿瘤研究所,国家肿瘤临床医学研究中心,天津市肿瘤防治重点实验室,天津300060 )
Author(s):
LIU Yuan YUAN Jie ZHANG Jie ZHAO Yuan-yuan NIU Rui-fang
(Cancer Institute and Hospital, Tianjin Medical University ,National Cancer Clinical Medicine Research Center ,Key Laboratory of Breast Cancer Prevention and Therapy ,Tianjin 300060,China)
关键词:
Ebp1 乳腺癌 增殖 侵袭
Keywords:
Ebp1 breast cancer proliferation invasion
分类号:
R737.9
DOI:
-
文献标志码:
A
摘要:
目的:研究降低Ebp1的基因表达水平对乳腺癌细胞T47D野生型增殖能力和侵袭能力的影响。方法:转染并筛选Ebp1降表达的单克隆细胞株clone1,clone2,clone3,阴性对照组命名为control。应用小干扰RNA技术降低Ebp1的表达,采用Western blotting技术检测Ebp1蛋白水平的变化,噻唑蓝(MTT)比色法和克隆形成实验观察其对T47D野生型细胞增殖能力和克隆形成能力的影响;采用Matrigel侵袭实验研究其对细胞侵袭能力的影响。结果:Western blotting检测表明siRNA干扰后T47D野生型细胞中Ebp1的蛋白表达水平明显下降,且与亲本和control组细胞比较,Ebp1降表达的细胞克隆的增殖能力和侵袭能力明显增强,差异有统计学意义(P<0.05)。结论:Ebp1表达下降可以明显促进乳腺癌细胞的增殖和侵袭能力,提示Ebp1可能是乳腺癌增殖和侵袭的抑制性因子。
Abstract:
Objective: To study the influence of the expression of Ebp1 on the proliferation and invasion of breast cancer cells. Methods: Western blotting and siRNA were applied to inspect and down-regulate the expression of Ebp1. The effect of proliferation and invasion of T47D wild type cells was examined by MTT assay, colony formation assay and transwell assay respectively. Results: The proliferation and invasion of T47D wild type could be promoted by Knock-down of Ebp1 as compared to wild type group and control group (P<0.05). Conclusion: Down-regulation of Ebp1 facilitates the proliferation and invasion of breast cancer cells which suggest that Ebp1 may be an inhibiting factor for breast cancer.

参考文献/References:

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备注/Memo

备注/Memo:

作者简介 刘媛(1987-),女,硕士在读,研究方向:乳腺癌增殖和侵袭的分子机制研究;

通信作者: 牛瑞芳,E- mail: niurfl982@yahoo.com.cn



 

更新日期/Last Update: 2015-06-10