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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

20卷

期数:

2014年03期

页码:

181-183

栏目:

基础医学

出版日期:

2014-05-20

文章信息/Info

Title:

Effects of rapamycin on liver cold ischemia/reperfusion injury in rats

文章编号:

1006-8147(2014)03-0181-03

作者:

徐 倩¹  ;  杜洪印² ;  喻文立² ;  翁亦齐² ;  丁 梅¹ ;  王玉亮³

(1．天津医科大学一中心临床学院麻醉科,天津300192;2．天津市第一中心医院麻醉科,天津300192;3．天津市卫生部危重病急救医学重点实验室,天津300384)

Author(s):

XU qian¹; DU Hong-yin²; YU Wen-li²; WENG Yi-qi²; DING mei¹; WANG Yu-liang³

(1．Department of Anesthesiology，The First Central Clinica1 College，Tianjin Medical University，Tianjin 300192，China；2．Department of Anesthesiology，The First Center Hospital of Tianjin，Tianjin 300192，China；3．The Key laboratory of Critical care of Medicine Ministry of Health，tianjin 300384，China)

关键词:

雷帕霉素; 肝脏; 缺血再灌注损伤; 大鼠

Keywords:

 ; rapamycin; liver; ischemia/reperfusion injury; rat

分类号:

-

DOI:

-

文献标志码:

A

摘要:

 目的：评价雷帕霉素（RAPA）对大鼠肝脏冷缺血再灌注损伤的影响。方法: 健康雄性SD大鼠24只随机分为3组，每组8只。假手术组（A组）大鼠仅接受麻醉后开腹，游离肝叶及相关血管后关腹；对照组（B组）采用肝脏冷缺血再灌注模型，分别采用门静脉插管和肝下下腔静脉缺口作为灌注道和流出道，用4℃乳酸林格氏液对肝脏进行冷灌注20 min；实验组（C组）大鼠术前连续3 d给予10 mg/(kg?d)雷帕霉素灌胃，手术操作同B组。3组大鼠均于再灌注24 h后经下腔静脉采血离心获取血清，测定血清中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）浓度及天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）的含量，取血后处死大鼠获取肝脏标本，用于检测肝组织中丙二醛（MDA）的含量及超氧化物歧化酶（SOD）的活性，光镜下观察肝组织病理学改变。结果：B 、C组血清中TNF-α和IL-6的浓度、AST、ALT的含量以及肝组织MDA的水平高于A组，而SOD活性降低（P<0.05）；C组除SOD活性比B组有所升高外，其余指标均低于B组（P<0.05）。A组肝脏组织形态结构未见异常，B、C组均见肝细胞损伤，C组损伤较B组减轻。结论：术前给予RAPA能减轻大鼠肝脏冷缺血再灌注损伤。

Abstract:

 Objective: To investigate the protective effects of rapamycin(RAPA) on liver cold ischemia/reperfusion injury in rats. Methods: Twenty-four healthy male SD rats were randomly divided into 3 groups. In sham operation group(A group), laparotomy was operated on anesthetized rats which only freed lobe and associated; Hepatic cold ischemia/reperfusion was performed in control group (B group)， in which a pipe was put in the portal vein to perfuse the liver using 4℃common Lactate Ringer’s solution，and the perfusion was discharged through a gap of infrahepatic inferior vena cava. After 20 minutes，the interdiction was removed；Rats in RAPA group(C group) were continuously given rapamycin lavage with a does of 10mg/(kg?d) three days before surgery, the same operation with group B. Blood samples were obtained from the inferior vena cava at 24 h of repefusion to detect the levels of serum tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6)，AST and ALT. Livers of all rats in three groups were removed to determine malondialdehyde (MDA) and superoxide dismutase (SOD) contents and examine the pathological changes in liver tissues(by light microscopy). Results: Compared with A group, the levels of serum TNF-α, IL-6, AST and ALT, and contents of MDA increased statistically in Groups B and C, and the SOD activity decreased (P<0.05). The SOD activity in Group C increased while other indices were lower than those in Group B (P<0.05). No significant pathological changes were found in liver tissues in group A. The injuries in Groups B and C were obvious marked with the injury in Group C significantly mitigated compared to Group B. Conclusion: RAPA can attenuate liver cold ischemia/reperfusion injury.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目  天津市卫生行业重点攻关项目 (12KG101), 国家高技术研究发展计划（2012AA021001）
作者简介 徐倩（1986-），女，硕士在读，研究方向：缺血再灌注肺损伤；通信作者：杜洪印，E-mail：duhongyin@medmail.com.cn。

常用功能

更新日期/Last Update: 2014-05-12