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[1]王玉华,张 峰,王亚林,等.基于网络药理学及分子对接探讨栀子治疗良性前列腺增生症的作用机制[J].天津医科大学学报,2026,32(02):153-159.[doi:10.20135/j.issn.1006-8147.2026.02.0153]
　WANG Yuhua,ZHANG Feng,WANG Yalin,et al.Exploring the mechanism of Gardeniae Fructus in the treatment of benign prostatic hyperplasia based on network pharmacology and molecular docking[J].Journal of Tianjin Medical University,2026,32(02):153-159.[doi:10.20135/j.issn.1006-8147.2026.02.0153]
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基于网络药理学及分子对接探讨栀子治疗良性前列腺增生症的作用机制(PDF)

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
32卷
期数:
2026年02期
页码:
153-159
栏目:
基础医学
出版日期:
2026-03-20

文章信息/Info

Title:
Exploring the mechanism of Gardeniae Fructus in the treatment of benign prostatic hyperplasia based on network pharmacology and molecular docking
文章编号:
1006-8147(2026)02-0153-07
作者:
王玉华; 张 峰; 王亚林; 李 军
（新乡医学院第三附属医院泌尿外科，新乡 453000）
Author(s):
WANG Yuhua;  ZHANG Feng;  WANG Yalin;  LI Jun
（Department of Urology， The Third Affiliated Hospital of Xinxiang Medical College， Xinxiang 453000，China）
关键词:
栀子; 良性前列腺增生症; 网络药理学; 分子对接
Keywords:
Gardeniae Fructus;  benign prostatic hyperplasia;  network pharmacology;  molecular docking
分类号:
R697+.32
DOI:
10.20135/j.issn.1006-8147.2026.02.0153
文献标志码:
A
摘要:
目的：通过网络药理学及分子对接探讨栀子治疗良性前列腺增生症的作用机制。方法：查询TCMSP筛选栀子的药物成分，使用Swiss Target Prediction预测药物成分的作用靶点；运用GeneCards获取良性前列腺增生症的疾病靶点，利用venny 2.1获取交集靶点。使用STRING进行蛋白质-蛋白质相互作用（PPI）分析并使用Cytoscape构建网络图，利用Metascape进行基因个体（GO）和京都基因与基因组百科全书（KEGG）分析。使用Cytoscape软件构建“药物-靶点-通路”网络图。选择 10 只雄性Sprague-Dawley大鼠，建立良性前列腺增生大鼠模型，按照随机数字表法随机分为对照组及治疗组，每组 5 只。治疗组以栀子溶液10 mL/（kg·d）（浓度为0.056 g/mL）进行灌胃治疗，对照组给予生理盐水灌胃，治疗后应用ELISA法检测两组动物模型丝裂原活化蛋白激酶（MAPK）1、丝氨酸/苏氨酸激酶（AKT）1、B淋巴细胞瘤2（BCL2）、前列腺素内过氧化物2（PTGS2）蛋白的表达。结果：研究发现，栀子的12个有效成分通过多条通路直接作用于67个疾病靶点，治疗良性前列腺增生症，其中槲皮素、山奈酚、β谷甾醇、豆甾醇等是核心成分，MAPK1、AKT1、BCL2、PTGS2是至关重要的靶点。基因功能注释分析结果显示，最可能与交集基因相关的生物过程主要涉及对激素的反应、细胞对脂质的反应、腺发育等，细胞组分主要涉及小窝、转录调控复合体、质膜筏等，分子功能主要涉及配体激活的转录因子活性、DNA结合转录因子结合、转录因子结合等。通路富集分析结果提示栀子主要通过晚期糖基化终末产物（AGE）-晚期糖基化终末产物受体（RAGE）、磷脂酰肌醇3激酶（PI3K）-AKT、低氧诱导因子（HIF）-1、MAPK等信号通路调节良性前列腺增生症。动物实验验证结果显示，与对照组相比，治疗后治疗组核心蛋白MAPK1、AKT1、BCL2、PTGS2表达均降低（t=18.910、12.585、20.056、14.569，均P<0.01）。结论：栀子主要通过调节AGE-RAGE、PI3K-AKT、HIF-1、MAPK等信号通路和MAPK1、AKT1、BCL2、PTGS2等疾病靶点，调节酶类活性、细胞死亡等生物过程而治疗良性前列腺增生症。
Abstract:
Objective： To explore the mechanism of Gardeniae Fructus in the treatment of benign prostatic hyperplasia （BPH） through network pharmacology and molecular docking. Methods： TCMSP was queried to screen the drug constituents of Gardenia and Swiss Target Prediction was used to predict the action targets of drug constituents. GeneCards was used to obtain disease targets for BPH， and venny 2.1 was used to obtain intersection targets. Protein-protein interaction（PPI） analysis was performedby STRING and network diagram was constructed by Cytoscape. Gene Ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） analysis were performed with Metascape. A drug-target-pathway network map was constructed using Cytoscape software. Ten male Sprague-Dawley rats were selected to establish a BPH model and were randomly assigned to either the control group or the treatment group， with five animals in each group. The treatment group received intragastric administration of gardenia solution at a dose of 10 mL/（kg·d）（concentration： 0.056 g/mL）， while the control group was administered normal saline via the same route. Following the intervention period， protein expression levels of mitogen-activated protein kinase（MAPK）1， serine/threonine-protein kinase （AKT）1， B-cell lymphoma 2 （BCL2）， and prostaglandin-endoperoxide synthase 2 （PTGS2） were quantified in both groups using enzyme-linked immunosorbent assay （ELISA）. Results： The study found that 12 active components of Gardeniae Fructus directly acted on 67 disease targets through multiple pathways to treat BPH. Querceti， kaempferol， β-sitosterol， and Stigmasteroare the core components， while MAPK1， AKT1， BCL2， PTGS2 were crucial targets. The results of gene function annotation analysis showed that the biological processes most likely related to crossover genes mainly involved the response to hormones， cell response to lipids， glandular development，etc. The cellular components mainly involved fossa， transcriptional regulatory complex， plasma membrane raft， etc. The molecular functions mainly involved ligand-activated transcription factor activity， DNA-binding transcription factor binding， transcription factor binding， etc. The results of pathway enrichment analysis suggested that Gardeniae Fructus mainly regulated BPH through advanced glycation end products （AGE）-receptor for advanced glycation end products （RAGE）， phosphatidylinositol 3-kinase （PI3K）-AKT， hypoxia inducible factor（HIF）-1， MAPK and other signaling pathways. The animal experimental validation results showed that compared with the control group，the expression of core proteins MAPK1， AKT1， BCL2， and PTGS2 were lower in the treatment group after treatment（t=18.910， 12.585， 20.056， 14.569， all P<0.01）. Conclusion： Gardeniae Fructus mainly regulates the signaling pathways of AGE-RAGE， PI3K-AKT， HIF-1， MAPK， disease targets such as MAPK1， AKT1， BCL2， PTGS2， enzyme activity， cell death and other biological processes to treat BPH.

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备注/Memo

备注/Memo:
作者简介 王玉华（1987-），主治医师，硕士，研究方向：泌尿系统肿瘤、泌尿道结石及前列腺疾病；通信作者：李军，E-mail：15836085628@ 139.com。
更新日期/Last Update: 2026-03-20