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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

29卷

期数:

2023年05期

页码:

514-520

栏目:

基础医学

出版日期:

2023-09-20

文章信息/Info

Title:

Mechanical loading promotes angiogenesis by up-regulating Piezo1 in disuse osteoporosis mice

文章编号:

1006-8147（2023）05-0514-07

作者:

孟尧¹; 王雪彤²; 李心乐¹; 张平¹; 3; 4

（1.天津医科大学基础医学院人体解剖与组织胚胎学系，天津 300070；2.天津医科大学基础医学研究中心，天津 300070；3. 卫生部激素与发育重点实验室，天津市代谢性疾病重点实验室，天津 300134；4. 天津市脊柱脊髓重点实验室，天津 300052）

Author(s):

MENG Yao¹; WANG Xue-tong²; LI Xin-le¹; ZHANG Ping¹; ³; ⁴

（1. Department of Anatomy and Histology，School of Basic Medical Sciences，Tianjin Medical University，Tianjin 300070，China; 2.Basic Medical Research Center，Tianjin Medical University，Tianjin 300070，China；3.Key Laboratory of Hormones and Development （Ministry of Health），Tianjin Key Laboratory of Metabolic Diseases，Tianjin 300134，China; 4.Tianjin Key Laboratory of Spine and Spinal Cord，Tianjin 300052，China）

关键词:

机械加载; 废用性骨质疏松; 血管生成; H血管; Piezo1

Keywords:

mechanical loading; disuse osteoporosis; angiogenesis; H vessels; Piezo1

分类号:

R68

DOI:

-

文献标志码:

A

摘要:

目的：探究机械加载通过调控Piezo1对废用性骨质疏松（DOP）小鼠血管形成的影响。方法：63只SPF级雌性C57BL/6小鼠按照随机数字表法分成假手术组（Sham组，n=21）、废用性骨质疏松组（DOP组，n=21）和废用性骨质疏松机械加载组（DOPL组，n=21）。通过鼠尾悬吊方式建立DOP小鼠模型，并使用本课题组自行研制的机械加载装置对小鼠进行为期2周的力学刺激治疗（条件为 1 N，5 Hz，6 min/d）。墨汁灌注和细胞免疫荧光实验观察骨血管生成情况，免疫组织化学染色和Western印迹方法检测DOP小鼠骨组织内Piezo1表达情况。通过沉默细胞内Piezo1来验证其对H血管生成的影响。结果：与 Sham组相比，DOP组骨量减少（t=9.553，P<0.001），而 DOPL组骨量增加（t=- 9.365，P<0.001）。同 Sham组相比，DOP 组 骨组织内血管下降（t=9.764，P<0.001）；而与DOP组相比，DOPL组骨组织内血管增加（t=10.001、9.764，均P<0.001），CD31和Emcn的表达升高（t=4.453、9.223，均P<0.01）。免疫组化显示，与DOP组相比，DOPL组Piezo1表达升高（t=6.557，P<0.01）；Western印迹分析结果表明，与DOP组相比，DOPL 组Piezo1表达增加（t=4.803，P<0.01）。沉默Piezo1降低了机械加载对H血管相关因子CD31和Emcn表达的促进作用。结论：机械加载可以通过促进Piezo1表达促进血管生成，抑制废用性骨质疏松小鼠骨量丢失。

Abstract:

Abstract Objective：To explore the effect of mechanical loading on angiogenesis in disuse osteoporosis（DOP） mice by regulating Piezo1. Methods：According to the method of random grouping，sixty-three SPF female C57BL/6 mice were divided into sham operation group（Sham group，n=21），disuse osteoporosis group（DOP group，n=21），and disuse osteoporosis mechanical loading group（DOPL group，n= 21）. The DOP mouse model was established by tail suspension，and the mechanical loading equipment which designed by our research group was used to treat the mice for two weeks（1 N，5 Hz，6 min/d）. The bone angiogenesis was observed by ink perfusion and cell immunofluorescence. The expression of Piezo1 was detected by immunohistochemical staining and Western blotting in the bone tissue of DOP mice. The effect of Piezo1 on H vessels was further verified by silencing intracellular Piezo1. Results：Compared with Sham group， bone mass in DOP group was decreased（t=9.553，P<0.001），while the bone mass of the DOPL group mice was increased（t=-9.365，P<0.001）. Compared with Sham group，the blood vessels in bone tissue of DOP group was decreased （t=9.764，P<0.001）. Compared with DOP group，DOPL group improved the decrease of blood vessel increase in bone tissue（t=10.001，9.764，both P<0.001），and increased the expressions of CD31 and Emcn（t=4.453，9.223，both P<0.01）. Compared with the DOP group，immunohistochemistry showed that the DOPL group enhances Piezo1 expression（t=6.557，P<0.01）. Western blotting analysis showed that，compared with the DOP group，the DOPL group further validated the increased Piezo1 expression（t=4.803，P<0.01）. Silencing Piezo1 reduced the promotion of mechanical loading on the expression of H vascular related factors CD31 and Emcn. Conclusion：Mechanical loading can promote angiogenesis and inhibit bone loss in disuse osteoporosis mice through Piezo1.

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金资助项目（81772405，81572100）
作者简介 孟尧（1997-），女，硕士在读，研究方向：骨质疏松的治疗机制研究；通信作者：张平，E-mail：pizhang2008@163.com。

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更新日期/Last Update: 2023-09-25