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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

28卷

期数:

2022年02期

页码:

140-144

栏目:

基础医学

出版日期:

2022-03-20

文章信息/Info

Title:

Palmitic acid-induced insulin resistance in mouse skeletal muscle cells reversed by dihydrotestosterone and its mechanism

文章编号:

1006-8147(2022)02-0140-05

作者:

齐睿; 牛文彦

（天津医科大学基础医学院免疫学系，天津300070）

Author(s):

QI Rui; NIU Wen-yan

（Department of Immunology，School of Basic Medical Sciences，Tianjin Medical University，Tianjin 300070，China）

关键词:

双氢睾酮; 骨骼肌; 胰岛素抵抗; 炎症; 糖尿病

Keywords:

dihydrotestosterone; skeletal muscle; insulin resistance; inflammation; diabetes mellitus

分类号:

R392.1

DOI:

-

文献标志码:

A

摘要:

目的：探讨双氢睾酮（DHT）对棕榈酸（PA）诱导的C2C12小鼠骨骼肌细胞胰岛素抵抗的影响及其分子机制。方法：分别用牛清蛋白（BSA）、PA或PA+DHT孵育C2C12细胞24 h，Western印迹检测胰岛素信号通路蛋白激酶B（Akt）、Akt底物AS160以及炎症相关蛋白核因子κB抑制蛋白（IκB）激酶（IKK）、核因子κB（NF-кB）的磷酸化水平、促炎细胞因子白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）蛋白水平，qPCR检测IL-6和TNF-α mRNA水平。结果：Western印迹结果显示，PA降低胰岛素刺激的Akt、AS160磷酸化水平（均P＜0.05）；DHT逆转PA对胰岛素刺激的Akt和AS160磷酸化的抑制作用（F=59.29、7.829；均P＜0.05）。PA升高IKK和NF-κB磷酸化水平（均P＜0.05）及IL-6和TNF-α蛋白表达（均P＜0.01）；DHT降低PA升高的IKK、NF-κB磷酸化水平（F=13.94、10.65，均P＜0.05）和IL-6和TNF-α蛋白表达（F=10.82、15.14，均P＜0.05）。qPCR结果显示，PA可升高IL-6、TNF-α mRNA水平（均P＜0.01）；DHT降低IL-6和TNF-α mRNA的表达（F=13.71、12.77；均P＜0.05）。结论：DHT可能通过抑制PA诱导的C2C12骨骼肌细胞炎症信号，改善PA诱导的胰岛素抵抗。

Abstract:

Objective： To investigate the effect of dihydrotestosterone （DHT） on palmitic acid（PA）-induced insulin resistance in C2C12 murine skeletal muscle cells and its potential molecular mechanism. Methods：C2C12 cells were incubated with bovine albumin （BSA），PA or PA+DHT for 24 hours. The phosphorylation levels of insulin signaling pathway related protein Akt，Akt substrate AS160 and inflammatory related protein IκB kinase（IKK），nuclear factor κB（NF-кB），pro-inflammatory cytokine interleukin-6（IL-6） and tumor necrosis factor-α（TNF-α） were detected by Western blotting.The mRNA levels of IL-6 and TNF-α were detected by qPCR. Results：Western blotting results showed that PA reduced insulin-stimulated Akt and AS160 phosphorylation levels（all P＜0.05）. DHT reversed the inhibitory effect of PA on insulin-stimulated Akt and AS160 phosphorylation（F=59.29，7.829；all P＜0.05）.PA increased the phosphorylation levels of IKK and NF-κB（all P＜0.05） and the protein expression of IL-6 and TNF-α（all P＜0.01）. DHT decreased the phosphorylation levels of IKK and NF-κB（F=13.94，10.65；all P＜0.05） and the expression of IL-6 and TNF-α（F=10.82，15.14；all P＜0.05）. The qPCR results showed that PA increased IL-6 and TNF-α mRNA level（both P <0.01）；DHT reduced the mRNA levels of IL-6 and TNF-α（F=13.71，12.77；both P＜0.05）. Conclusion：DHT may reverse PA-induced insulin resistance by inhibiting inflammation signal in C2C12 skeletal muscle cells.

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备注/Memo

备注/Memo:

基金项目 天津市教委科研计划项目（2019ZD028）
作者简介 齐睿（1997-），女，硕士在读，研究方向：免疫学；通信作者：牛文彦，E-mail：wniu@tmu.edu.cn。

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更新日期/Last Update: 2022-03-20