|本期目录/Table of Contents|

[1]程雅静,刘莹,王菲.基于生物信息学方法筛选散发性克雅氏病神经炎症相关关键基因[J].天津医科大学学报,2022,28(01):1-7.
　CHENG Ya-Jing,LIU Ying,WANG Fei.Identification of hub genes related to neuroinflammation of sporadic Creutzfeldt-Jakob disease by integrated bioinformatics analysis[J].Journal of Tianjin Medical University,2022,28(01):1-7.
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基于生物信息学方法筛选散发性克雅氏病神经炎症相关关键基因(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
28卷
期数:
2022年01期
页码:
1-7
栏目:
生物信息学专题
出版日期:
2022-01-20

文章信息/Info

Title:
Identification of hub genes related to neuroinflammation of sporadic Creutzfeldt-Jakob disease by integrated bioinformatics analysis
文章编号:
1006-8147（2022）01-0001-07
作者:
程雅静; 刘莹; 王菲
（天津医科大学总医院神经内科，天津300052）
Author(s):
CHENG Ya-Jing; LIU Ying; WANG Fei
（Department of Neurology，The General Hospital，Tianjin Medical University，Tianjin 300052，China）
关键词:
散发性克雅氏病; 神经炎症; 生物信息学; 关键基因
Keywords:
sporadic Creutzfeldt-Jakob disease; neuroinflammation; bioinformatics; key genes
分类号:
R742.8+9
DOI:
-
文献标志码:
A
摘要:
目的：鉴定散发性克雅氏病（SCJD）神经炎症相关的关键基因。方法:用GEO2R工具筛选GSE160208数据集中的SCJD脑组织（n=27）和正常脑组织（n=20）的差异表达基因（DEGs）。用R语言的“cluster Profiler”和“DOSE”包对DEGs进行富集分析。通过STRING数据库和Cytoscape 软件构建蛋白质相互作用（PPI）网络并筛选关键基因。用GSE124571数据集验证关键基因的表达，绘制关键基因的受试者工作特征（ROC）曲线鉴定hub genes。结果：本研究有127个DEGs，富集分析结果提示DEGs与吞噬体、核因子-κB信号通路和B细胞受体信号通路等有关。经过PPI 网络的构建、hub基因RNA表达的验证和ROC曲线的绘制，最终TLR2、 C1QA、 ITGAM、 C3AR1在SCJD中表达升高（t=2.12、3.197、3.107、3.284，均P＜0.05），对SCJD有较高的诊断效能（曲线下面积＞0.9），被筛选为SCJD hub基因。结论：TLR2、 C1QA、 ITGAM、 C3AR1在SCJD中有关键作用，可以作为SCJD发展过程中神经炎症相关的hub基因。
Abstract:
Objective：To investigate the key genes related to neuroinflammation of sporadic Creutzfeldt-Jakob disease（SCJD）. Methods: GEO2R was used to screen the differentially expressed genes（DEGs） between SCJD brain tissue （n=27） and normal brain tissue（n=20） from the GSE160208 dataset，and functional enrichment analysis of DEGs was performed using "cluster Profiler" and "DOSE" packages in the R language. Then，a protein-protein interaction（PPI） network of the DEGs was established by the STRING database and Cytoscape to screen key genes. The GSE124571 dataset was used to verify the expression of key genes，and ROC curves of key genes were drawn to identify hub genes of SCJD. Results: The results of enrichment analysis of 127 DEGs suggest that DEGs were related to phagosome，neuclear factor-κB signal pathway and B cell receptor signal pathway. After constructing of PPI network，verifying of GSE124571 dataset and drawing ROC curves，TLR2，C1QA，ITGAM，and C3AR1 are highly expressed in SCJD（t=2.12，3.197，3.107， 3.284，all P＜0.05） and had highly diagnostic efficiency for SCJD（area under curve＞0.9），so they were selected as hub genes of SCJD. Conclusion: TLR2，C1QA，ITGAM and C3AR1 play key roles in SCJD and can be used as hub genes related to neuroinflammation in the development of SCJD.

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金（81671054，81771135）；基因工程教育部重点实验室开放基金（201503）；天津市应用基础与前沿技术重点项目（19JCZDJC35600）；天津市中青年骨干创新人才培养计划（303078100407）
作者简介 程雅静（1995-），女，硕士在读，研究方向：神经病学；通信作者:王菲，E-mail: feiwang@tmu.edu.cn。
更新日期/Last Update: 2022-01-20