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[1]乌日吉木斯,刘啸轩,苏悦,等.白细胞介素-18在小鼠缺血性脑卒中的损伤作用及其机制[J].天津医科大学学报,2021,27(06):603-607.
　WU Ri-jimusi,LIU Xiao-xuan,SU Yue,et al.The injury effect of interleukin-18 in the mice with ischemic stroke and its mechanism[J].Journal of Tianjin Medical University,2021,27(06):603-607.
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白细胞介素-18在小鼠缺血性脑卒中的损伤作用及其机制(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
27卷
期数:
2021年06期
页码:
603-607
栏目:
基础医学
出版日期:
2021-11-15

文章信息/Info

Title:
The injury effect of interleukin-18 in the mice with ischemic stroke and its mechanism
文章编号:
1006-8147（2021）06-0603-05
作者:
乌日吉木斯1; 刘啸轩1; 苏悦1; 阎涛1; 2
（天津医科大学总医院1.神经病学研究所；2.神经内科，天津300052）
Author(s):
WU Ri-jimusi1; LIU Xiao-xuan1; SU Yue1; YAN Tao1; 2
（1. Tianjin Neurological Institute；2. Department of Neurology，General Hospital，Tianjin Medical University, Tianjin 300052, China）
关键词:
缺血性脑卒中; 白细胞介素-18; 炎症; 小胶质细胞; 小鼠
Keywords:
ischemic stroke; interleukin-18; inflammation; microglia; mice
分类号:
R743.3
DOI:
-
文献标志码:
A
摘要:
目的：探讨白细胞介素（IL）-18在小鼠缺血性脑卒中的损伤作用及其机制。方法：采用C57BL/6J成年雄性小鼠构建光化学法诱导局灶性大脑皮层缺血性脑卒中模型，随机分为对照组、脑梗死组、IL-18分泌型结合蛋白（IL-18BP）治疗组，每组18只。术后14 d进行神经功能评分并进行HE染色测量小鼠脑梗死面积。术后3 d实时荧光定量PCR检测小鼠脑组织中肿瘤坏死因子-α（TNF-α）、IL-1β、IL-6和IL-10的表达，流式细胞术检测小鼠脑组织中小胶质细胞表型。结果：与脑梗死组相比，IL-18BP治疗组神经功能显著改善并且脑梗死面积显著降低（t=2.750、2.235，均P<0.05）。IL-18BP治疗组脑组织中TNF-α、IL-1β和IL-6的表达显著降低（t=3.091、2.328、3.443，均P<0.05），IL-10的表达升高（t=4.997，P<0.05）。与脑梗死组相比，IL-18BP治疗组脑组织中M1型小胶质细胞数量明显降低（t=4.779，P<0.05），M2型小胶质细胞数量明显升高（t=2.619，P<0.05）。结论：在缺血性脑卒中，IL-18可介导免疫炎性损伤并发挥调节作用。
Abstract:
Objective:To investigate the injury effect of interleukin-18（IL-18） in mice with ischemic stroke and its mechanism. Methods: C57BL/6J adult male mice were used to construct a photochemically induced ischemic stroke model，and they were randomly divided into sham group，stroke group，and IL-18BP （IL-18 secretion bound protein） treatment group，18 mice in each group. Neurological function score was performed 14 days after operation，and cerebral infarct size was measured by H&E staining. The expression of inflammatory factors such as tumor necrosis factor-α（TNF-α），interleukin-1β（IL-1β），interleukin-6（IL-6） and interleukin-10（IL-10）in the brain tissues of mice was detected by Quantitative Real-time PCR 3 days after operation，and the microglial cell phenotype in the brain tissues of mice was detected by flow cytometry. Results: Compared with stroke group，neurological function was significantly improved and cerebral infarct size was significantly reduced（t=2.235，P<0.05）in the IL-18BP treatment group.The expression of TNF-α，IL-1β and IL-6 in the IL-18BP treatment group was significantly decreased（t=3.091，2.328，3.443，all P<0.05），while the expression of IL-10 was increased（t=4.997，P<0.05）.Compared with stroke group，the number of M1-type microglia was significantly decreased（t=4.779，P<0.05） and the number of M2 microglia was significantly increased（t=2.619，P<0.05） in the IL-18BP treatment group. Conclusion: In ischemic stroke，IL-18 mediates immune inflammatory injury and plays a key regulatory role.

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备注/Memo

备注/Memo:
基金项目 天津市自然科学基金重点项目（17JCZDJC36100） 作者简介 乌日吉木斯（1996-），女，硕士在读，研究方向：神经病学；通信作者：阎涛，E-mail：taoyan@tmu.edu.cn。
更新日期/Last Update: 2021-11-15