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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

26卷

期数:

2020年05期

页码:

422-428

栏目:

基础医学

出版日期:

2020-09-20

文章信息/Info

Title:

Effect of insulin treatment on miR-126, VEGF and eNOS expression in penile tissues of diabetic rats

文章编号:

1006-8147(2020)05-0422-07

作者:

胡睿¹; 张威¹; 欧宁静¹; 梁震¹; 杨永姣²; 刘莉¹; 刘晓强¹

（1.天津医科大学总医院泌尿外科，天津300052；2.天津医科大学第二医院泌尿外科，天津市泌尿外科研究所，天津 300211）

Author(s):

HU Rui¹;  ZHANG Wei¹;  OU Ning-jing¹;  LIANG Zhen¹;  YANG Yong-jiao²;  LIU Li¹;  LIU Xiao-qiang¹

（1. Department of Urology, General Hospital, Tianjin Medical University, Tianjin 300052, China; 2. Department of Urology, The Second Hospital， Tianjin Medical University, Tianjin Institute of Urology, Tianjin 300211, China）

关键词:

糖尿病性勃起功能障碍; 胰岛素; miR-126; VEGF; eNOS

Keywords:

diabetes associated erectile dysfunction;  insulin;  miR-126;  VEGF;  eNOS

分类号:

R698+R587.2

DOI:

-

文献标志码:

A

摘要:

目的：研究胰岛素治疗对糖尿病大鼠阴茎组织中miR-126、VEGF、eNOS表达的影响。方法：8周龄雄性Sprague-Dawley（SD）大鼠30只，采用随机数字表将其分为正常对照组（NC组）、糖尿病组 （DM组）和胰岛素治疗组（Insulin组）。腹腔内注射链脲佐菌素（STZ）建立糖尿病（DM）大鼠模型，Insulin组于成模后开始注射胰岛素，连续8周。治疗结束后，检测各组大鼠的体重、血糖、阴茎海 绵体内压（ICP）、平均颈动脉内压（MAP）及ICP与MAP比值（ICP/MAP），阴茎海绵体中内皮型一氧化氮合酶（eNOS）、血管内皮生长因子（VEGF）及miR-126的表达量。结果：胰岛素治疗后，Insulin组 的体重较DM组明显增加（t=-10.481，P<0.001），血糖较DM组显著降低（t=4.356，P<0.001）；Insulin组的ICP/MAP较DM组明显增加（t=-14.349，P<0.001）；Western印迹结果显示，Insulin组阴茎海 绵体中eNOS及VEGF蛋白表达量均较DM组显著增加（t=-6.786，-21.177，均P<0.001）。RT-PCR结果显示，Insulin组阴茎海绵体中eNOS、VEGF和miR-126的表达量均较DM组显著增加（t=-6.994、- 8.238、-10.665，均P<0.001）。但上述指标均未达到NC组水平。结论：胰岛素治疗可增加阴茎组织中miR-126、VEGF及eNOS的表达水平。

Abstract:

Objective: To investigate the effect of insulin treatment on miR-126, VEGF and eNOS expression in penile tissues of diabetic rats. Methods: 30 male Sprague-Dawley (SD) rats aged 8 weeks were randomly divided into three groups by random number table: normal control group(NC group), diabetes mellitus group (DM group) and insulin treatment group(Insulin group). Intraperitoneal injection of streptozotocin(STZ) to establish a rat model of diabetes mellitus (DM). Insulin group were started to inject insulin for 8 weeks after the DM model established. When the treatment was finished, the body weight, blood glucose, intracorporeal pressure(ICP), mean arterial pressure(MAP) and the ratio of ICP and MAP (ICP/MAP) of rats in different groups were measured. The expressions of eNOS, VEGF and miR-126 in the penile tissues were detected. Results: After 8 weeks of insulin treatment, the body weight of Insulin group significantly increased compared with DM group (t=-10.481, P<0.001), while the blood glucose significantly decreased compared with DM group(t=4.356, P<0.001). The ICP/MAP of Insulin group significantly increased compared with DM group (t=-14.349, P<0.001).The expressions of eNOS and VEGF in Insulin group by Western blot were significantly increased compared with DM group(t=-6.786, -21.177, both P<0.001). The expressions of eNOS, VEGF and miR-126 in Insulin group by RT-PCR were significantly increased compared with DM group(t=-6.994, -8.238, -10.665, all P<0.001). However, the above results were not equal to the level of NC group. Conclusion: Insulin treatment can increase the expressions of miR-126, VEGF and eNOS in penile tissues.

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备注/Memo

备注/Memo:

基金项目 赵以成医学科学基金资助项目(ZYYFY2018031)
作者简介 胡睿(1993-)，男，硕士在读，研究方向：男科疾病的临床和基础研究；
通信作者：刘晓强，E-mial：liuxiaoqiangtjykdx@163.com。

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更新日期/Last Update: 2020-09-18