|本期目录/Table of Contents|

[1]黄金灿,张磊,常永生.KLF9基因腺病毒载体的构建及其脂肪酸氧化功能[J].天津医科大学学报,2020,26(03):193-198.
　HUANG Jin-can,ZHANG Lei,CHANG Yong-sheng.KLF9 adenoviral vector construction and it’s function in fatty acid oxidation[J].Journal of Tianjin Medical University,2020,26(03):193-198.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
26卷
期数:
2020年03期
页码:
193-198
栏目:
基础医学
出版日期:
2020-06-10

文章信息/Info

Title:
KLF9 adenoviral vector construction and it’s function in fatty acid oxidation
文章编号:
1006-8147(2020)03-0193-06
作者:
黄金灿; 张磊; 常永生
（天津医科大学基础医学院生理与病理生理学系，天津300070）
Author(s):
HUANG Jin-can; ZHANG Lei; CHANG Yong-sheng
（Department of Pathology and Physiology， School of Basic Medicine，Tianjin Medical University，Tianjin 300070，China)
关键词:
KLF9; 脂肪酸氧化; PGC1-α; 腺病毒; 肝脏
Keywords:
KLF9; fatty acid oxidation; PGC1-α; adenovirus; liver
分类号:
R363
DOI:
-
文献标志码:
A
摘要:
目的：构建Krüppel样转录因子9（KLF9）过表达的腺病毒，初步探讨KLF9在肝脏脂肪酸氧化中的功能。方法：根据分子克隆的原理，构建融合表达3×Flag的KLF9过表达的腺病毒，并在293A细胞中验证KLF9腺病毒感染效率。将融合表达3×Flag的KLF9过表达的腺病毒分别感染肝原代细胞、正常饮食小鼠以及高脂小鼠，通过Q-PCR检测过氧化物酶体增殖物活化受体-γ协同刺激因子PGC 1-α等脂肪酸氧化相关基因的表达情况。结果：Q-PCR以及 Western blot 结果证明融合表达3×Flag的KLF9过表达腺病毒载体包装成功，通过荧光显微镜观察到感染效率达90%以上。经Q-PCR检测，感染Ad-KLF9的肝原代细胞和肝脏组织中，PGC1-α等脂肪酸氧化相关基因表达明显高于对照组。结论：初步认定KLF9可促进脂肪酸氧化，改善高脂诱导的非酒精性脂肪肝。
Abstract:
Objective: To construct Krüppel-like transcription factor 9(KLF9) overexpressed adenovirus and explore the role of KLF9 in liver fatty acid oxidation. Methods：KLF9 fusion with 3×Flag overexpressed adenovirus was constructed, according to the principle of molecular cloning, and the infection efficiency of adenovirus was tested in 293A cells. The Ad-KLF9 was infected into primary liver cells, chow and high-fat diet induced mice respectively, and the expression of fatty acid oxidation related genes such as PGC1-α were detected by Q-PCR. Results：Q-PCR and Western blot results showed that KLF9 fusion with 3×Flag overexpressed adenovirus was successfully packaged, and more than 90% of the infection efficiency was observed by fluorescence microscopy. The expression of fatty acid oxidation related genes such as PGC1-α in primary liver cells and liver tissues infected with Ad-KLF9 were significantly higher than those in the control group by Q-PCR. Conclusion：It is preliminarily determined that KLF9 can promote fatty acid oxidation and improve the non-alcoholic fatty liver disease.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金资助项目（817300024）
作者简介 黄金灿（1994-），女，硕士在读，研究方向：医学生理学；通信作者：常永生，E-mail:changy@ibms.pumc.en。
更新日期/Last Update: 2020-06-13