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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

25卷

期数:

2019年06期

页码:

605-608+614

栏目:

临床医学

出版日期:

2019-11-20

文章信息/Info

Title:

Relationship between Tim-3, IL-17 and recurrent spontaneous abortion

文章编号:

1006-8147(2019)06-0605-05

作者:

李萌萌; 杨小风; 刘 芳; 武明莉; 张 芳

（郑州大学附属郑州中心医院妇产科, 郑州 450000）

Author(s):

LI Meng-meng;  YANG Xiao-feng;  LIU Fang;  WU Ming-li;  ZHANG Fang

（Department of Obstetrics and Gynecology， Zhengzhou Central Hospital Affiliated ,Zhengzhou University，Zhengzhou 450000，China)

关键词:

TIM-3; RSA; IL-17; 单核细胞

Keywords:

TIM-3;  RSA;  IL-17;  monocyte

分类号:

R714.21

DOI:

-

文献标志码:

A

摘要:

目的：探讨T细胞免疫球蛋白黏蛋白分子-3（Tim-3）在复发性流产（RSA）发病机制中的作用。方法：选取2017年12月-2018年7月郑州市中心医院妇产科收治的复发性流产患者36例为RSA组，同期23例健康早孕妇女作为正常早孕组（NP组），29例仅有少量阴道出血的早孕妇女为先兆性流产组（TA组）。提取分离外周血单核细胞，流式细胞术检测3组研究对象外周血中CD4+Tim-3+T细胞；ELISA检测3组研究对象血清中IL-17的表达；Pearson 相关分析TA组和RSA组患者CD4+Tim-3+T细胞与 IL-17 的相关性；Western blot检测RSA组和NP组自愿人工流产患者绒毛组织中滋养细胞Tim-3蛋白的含量。结果：与NP组相比，TA组和RSA组外周血单核细胞上Tim-3表达降低，外周血IL-17表达增高（P<0.05）；相关性分析提示TA组及RSA组外周血单核细胞上 Tim-3与IL-17之间呈负相关；NP组滋养细胞Tim-3蛋白的含量高于RSA组。结论：外周血单核细胞上 Tim-3可能通过调节 IL-17间接调节Th17/Treg细胞参与先兆流产和RSA的发生。

Abstract:

Objective: To investigate the role of T cell immunoglobulin mucin-3 (Tim-3) in the pathogenesis of recurrent spontaneous abortion (RSA). Methods: From December 2017 to July 2018, 36 patients with recurrent spontaneous abortion admitted to the Department of Obstetrics and Gynecology in Zhengzhou Central Hospital were included in the RSA group. In the same period, 23 healthy early pregnant women were used as the normal early pregnancy group, and 29 patients who had only a small amount of vaginal bleeding are a threatened abortion group. Peripheral blood mononuclear cells were isolated by density gradient centrifugation. CD4+Tim-3+ T cells were detected by flow cytometry in peripheral blood of the three groups. The expression of IL-17 in serum of the three groups was detected by ELISA. Pearson correlation analysis correlation between CD4+Tim-3+ T cells and IL-17 in RSA patients; Western Blot was used to determine the contents of Tim-3 protein in villus tissue of RSA group and NP group. Results: Compared with the NP group, the expression of Tim-3 decreased in peripheral blood mononuclear cells of the TA group and RSA group, and the expression of IL-17 in peripheral blood increased(P<0.05). Correlation analysis showed that there was a negative correlation between Tim-3 and IL-17 in peripheral blood mononuclear cells of TA group and RSA group. The content of Tim-3 protein in villus cells of induced abortion patients with early pregnancy was higher than that of RSA group. Conclusion: Tim-3 may regulate the involvement of Th17/Treg cells in TA and RSA by regulating the IL-17 in peripheral blood mononuclear cells.

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备注/Memo

备注/Memo:

基金项目 河南省教育厅2018年度重点科研项目（18B310036）
作者简介 李萌萌（1987-），女，住院医师，硕士，研究方向：生殖内分泌；通信作者：杨小风，E-mail：ta57di@163.com。

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更新日期/Last Update: 2020-01-20