|本期目录/Table of Contents|

[1]吕晓婷,洪宇桁,牛文彦.短链脂肪酸对C2C12小鼠骨骼肌细胞AMPK的作用研究[J].天津医科大学学报,2019,25(01):1-4，23.
　LV Xiao-ting,HONG Yu-heng,NIU Wen-yan.Effect of short-chain fatty acids on AMPK in mouse C2C12 skeletal muscle cells[J].Journal of Tianjin Medical University,2019,25(01):1-4，23.
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短链脂肪酸对C2C12小鼠骨骼肌细胞AMPK的作用研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
25卷
期数:
2019年01期
页码:
1-4，23
栏目:
基础医学
出版日期:
2019-01-20

文章信息/Info

Title:
Effect of short-chain fatty acids on AMPK in mouse C2C12 skeletal muscle cells
文章编号:
1006-8147(2019)01-0001-04
作者:
吕晓婷1; 洪宇桁2; 牛文彦1
（1.天津医科大学免疫学系，天津300070；2.天津医科大学医学影像学院，天津300203）
Author(s):
LV Xiao-ting1;  HONG Yu-heng2;  NIU Wen-yan1
（1.Department of Immunology, Tianjin Medical University, Tianjin 300070, China; 2.School of Medical Imaging ,Tianjin Medical University, Tianjin 300203, China）
关键词:
骨骼肌; 短链脂肪酸; AMPK
Keywords:
skeletal muscle;  SCFAs;  AMPK
分类号:
R392.1
DOI:
-
文献标志码:
A
摘要:
目的：探讨短链脂肪酸对C2C12小鼠骨骼肌细胞AMPK的作用。方法：分别用不同浓度的乙酸钠、丙酸钠和丁酸钠孵育C2C12细胞24 h，MTS试验检测细胞活力，Western blot检测AMPK磷酸化水平。结果：与对照组相比，1 mmol/L和4 mmol/L乙酸钠，4 mmol/L丙酸钠以及4 mmol/L、8 mmol/L和16 mmol/L丁酸钠升高AMPK磷酸化水平，但不影响其总蛋白水平。结论：短链脂肪酸激活C2C12小鼠骨骼肌细胞AMPK。
Abstract:
Objective: To investigate the effect of SCFAs on AMPK in mouse C2C12 skeletal muscle cells. Methods: C2C12 cells were incubated with different concentrations of sodium acetate, sodium propionate and sodium butyrate for 24 hours, respectively. The cell viability was measured by MTS assay. The phosphorylation of AMPK was detected by western blot. Results: Compared with the control group, the phosphorylation of AMPK increased under treatments with 1 mmol/L and 4 mmol/L sodium acetate, 4 mmol/L propionate and 4 mmol/L, 8 mmol/L, and 16 mmol/L butyrate, respectively. The total protein level of AMPK was not affected. Conclusion: SCFAs phosphorylate AMPK in mouse C2C12 skeletal muscle cells.

参考文献/References:


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备注/Memo

备注/Memo:
基金项目 国家自然科学基金面上项目（81670731）；天津市科委应用基础研究重点项目（15JCZDJC35500）；天津市卫计委重点攻关项目（15KG102 ）
作者简介 吕晓婷（1992-），女，硕士在读，研究方向：免疫学；通信作者：牛文彦，E-mail:wniu@tmu.edu.cn。
更新日期/Last Update: 2019-03-01