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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

24卷

期数:

2018年03期

页码:

188-191

栏目:

出版日期:

2018-05-20

文章信息/Info

Title:

Detection of microRNA-21 up-expression in tumor cells with DNA-templated silver nanocluster probe

作者:

侯肖肖¹; 孙丽范¹; 樊明玥²; 孙艳华¹

天津医科大学1. 药学院；2.公共卫生学院，天津300070

Author(s):

HOU Xiao-xiao¹;  SUN Li-fan¹;  FAN Ming-yue²;  SUN Yan-hua¹

1. School of Pharmacy; 2. School of Public Health, Tianjin Medical University, Tianjin 300070, China

关键词:

肿瘤标志物; 纳米银簇; microRNA-21; 荧光性质

Keywords:

tumor markers;  AgNCs;  microRNA-21;  fluorescence properties

分类号:

R9

DOI:

-

文献标志码:

A

摘要:

目的：设计由DNA模板引导制备的纳米银簇探针，实现对肿瘤细胞中高表达的microRNA-21的定性和定量检测。方法：利用荧光光谱、紫外-可见吸收光谱、圆二色谱以及高分辨电镜表征技术，探究DNA引导合成纳米银簇的性质。通过计算血清回收率，验证探针对microRNA-21的检测效率。结果：以DNA为模板合成的纳米银簇探针能成功检测肿瘤细胞高表达的microRNA-21。结论：建立了microRNA-21的检测方法，并为临床检测肿瘤标志物提供了思路。

Abstract:

Objective: To detect the up-expressed microRNA-21 in tumor cells qualitatively and quantitatively using designed DNA-templated silver nanoclusters(DNA/AgNCs) probe. Methods: Fluorescence spectroscopy, UV-Vis absorption spectroscopy, circular dichroism spectroscopy and high-resolution electron microscopy were employed to study properties of DNA/AgNCs probes. The detection efficiency for microRNA-21 was tested by the calculating serum recovery. Results: The up-expressed microRNA-21 in tumor cells was detected by DNA/AgNCs probe successfully. Conclusion: A method for the detection of microRNA-21 is established and it could be used for clinical detection of tumor biomarkers.

参考文献/References:

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[2] Volinia S, Calin G A, Liu C G, et al. A microRNA expression signature of human solid tumors defines cancer gene targets[J]. Proc Natl Acad Sci U S A, 2006, 103(7): 2257
[3] Wang Q, Liu S, Tang Y, et al. MPT64 protein from Mycobacterium tuberculosis inhibits apoptosis of macrophages through NF-kB-miRNA21-Bcl-2 pathway[J]. PLoS One, 2014, 9(7): e100949
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[8] Lee S Y, Hairul Bahara N H, Choong Y S, et al. DNA fluorescence shift sensor: a rapid method for the detection of DNA hybridization using silver nanoclusters[J]. J Colloid Interface Sci, 2014, 433: 183.
[9] He K, Liao R, Cai C, et al. Y-shaped probe for convenient and label-free detection of microRNA-21 in vitro[J]. Anal Biochem, 2016, 499: 8
[10] Lee J D, Cang J, Chen Y C, et al. Detection of adenosine 5'-triphosphate by fluorescence variation of oligonucleotide-templated Silver nanoclusters[J]. Biosens Bioelectron, 2014, 58: 266
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[12] Zhu Y, Hu X C, Shi S, et al. Ultrasensitive and universal fluorescent aptasensor for the detection of biomolecules (ATP, adenosine and thrombin) based on DNA/Ag nanoclusters fluorescence light-up system[J]. Biosens Bioelectron, 2016, 79: 205

相似文献/References:

[1]白惠惠,邓靖宇,梁 寒.血清肿瘤标志物对胃癌淋巴结转移的诊断价值[J].天津医科大学学报,2019,25(03):241.
　BAI Hui-hui,DENG Jing-yu,LIANG Han.Prognostic value of serum tumor biomarkers for lymph node metastasis of gastric cancer[J].Journal of Tianjin Medical University,2019,25(03):241.

备注/Memo

备注/Memo:

文章编号 1006-8147(2018)03-0188-04
基金项目 国家自然科学基金资助项目(31200753)
作者简介 侯肖肖(1989-)，女，硕士在读，研究方向:DNA调控纳米银簇应用于microRNA检测；通信作者：孙艳华,E-mail:sunyh@tmu.edu.cn。

常用功能

更新日期/Last Update: 2018-05-20