|本期目录/Table of Contents|

[1]卢娜,方步武.蒿鳖养阴软坚方对刀豆球蛋白A诱导的肝纤维化Nrf2/HO-1信号通路影响[J].天津医科大学学报,2018,24(02):112-115,121.
　LU Na,FANG Bu-wu.Effect of Haobie yangyin ruanjian prescription on Nrf2/HO-1 signaling pathway in ConA-induced hepatic fibrosis[J].Journal of Tianjin Medical University,2018,24(02):112-115,121.
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蒿鳖养阴软坚方对刀豆球蛋白A诱导的肝纤维化Nrf2/HO-1信号通路影响(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
24卷
期数:
2018年02期
页码:
112-115,121
栏目:
出版日期:
2018-03-20

文章信息/Info

Title:
Effect of Haobie yangyin ruanjian prescription on Nrf2/HO-1 signaling pathway in ConA-induced hepatic fibrosis
作者:
卢娜; 方步武
天津医科大学药理学系，天津 300070
Author(s):
LU Na;  FANG Bu-wu
Department of Pharmacology, Tianjin Medical University, Tianjin 300070,China
关键词:
肝纤维化; 蒿鳖养阴软坚方; 刀豆球蛋白A; Nrf2; HO-1
Keywords:
hepatic fibrosis;  Haobie yangyin ruanjian prescription;  ConA;  Nrf2;  HO-1
分类号:
R575
DOI:
-
文献标志码:
A
摘要:
目的：探讨蒿鳖养阴软坚方对刀豆球蛋白A（ConA）诱导的肝纤维化Nrf2/HO-1信号通路的影响。方法：Balb/c小鼠随机分为正常、模型、秋水仙碱和蒿鳖养阴软坚方低、中、高剂量(1.16 g/kg、3.36 g/kg、11.58 g/kg)组。除正常组外，剩余各组均尾静脉注射1.5 mg/mL ConA，每周1次，持续10周。取小鼠肝脏作病理切片，观察肝脏的病理变化，检测肝组织羟脯氨酸浓度，免疫组化法检测肝组织Nrf2、HO-1蛋白的表达情况。结果：与模型组比较，蒿鳖养阴软坚方各剂量组能不同程度地减轻肝组织的炎症反应和降低肝组织羟脯氨酸的浓度，显著减轻肝纤维化程度（P＜0.05）；随着蒿鳖养阴软坚方浓度增加，Nrf2和HO-1的阳性表达逐渐增强。结论：蒿鳖养阴软坚方对Nrf2/HO-1信号通路具有激活作用，且该作用可能为其抗肝纤维化机制之一。
Abstract:
Objective: To explore the effect of Haobie yangyin ruanjian prescription (HYRP) on Nrf2/HO-1 signaling pathway in ConA-induced hepatic fibrosis. Methods: Balb/c mice were randomly divided into normal control group, model control group, colchicine group, and HYRP groups(1.16 g/kg，3.36 g/kg，11.58 g/kg). Except for the normal control group, 1.5 mg/mL ConA was given to each mouse through the tail vein once a week for 10 weeks. The concentration of hydroxyproline（Hyp）and observing liver pathological changes in liver tissues were measured, and the expression of Nrf2 and HO-1 protein in liver tissues were detected by immunohistochemistry. Results: Compared with model control group, groups of 1.16 g/kg，3.36 g/kg and 11.58 g/kg of HYRP could reduce the inflammatory reaction and decrease the concentration of Hyp in liver tissues, and significantly reduce the degree of hepatic fibrosis (P＜0.05). With the increase of HYRP concentration，the positive expressions of Nrf2 and HO-1 gradually increased. Conclusion: HYRP can activate the expression of Nrf2/HO-1 signaling pathway in liver fibrosis induced by ConA, which may be related the anti-fibrotic effects of HYRP.

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备注/Memo

备注/Memo:
文章编号 １００６－８１４７（２０18）０2-0112-04
作者简介 卢娜（1992-），女，硕士在读，研究方向：中药药理；通信作者：方步武，E-mail：fangdch@aliyun.com。
更新日期/Last Update: 2018-03-20