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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

24卷

期数:

2018年02期

页码:

93-96

栏目:

出版日期:

2018-03-20

文章信息/Info

Title:

Identification of DAMPs and PAMPs by Siglec-G in macrophages and its regulatory effect on DAMPs and PAMPs in inflammatory response

作者:

樊亚童; 郑鉴; 肖俊; 张学军

天津医科大学免疫学系，天津300070

Author(s):

FAN Ya-tong;  ZHENG Jian;  XIAO Jun;  ZHANG Xue-jun

Department of Immunology, Tianjin Medical University, Tianjin 300070, China

关键词:

DAMPs; PAMPs; 巨噬细胞; Siglec-G

Keywords:

PAMPs;  DAMPs;  macrophage;  Siglec-G

分类号:

R392.1

DOI:

-

文献标志码:

A

摘要:

目的：探究巨噬细胞中唾液酸结合性免疫球蛋白样凝集素（Siglecs）家族的成员Siglec-G参与损伤相关分子模式（DAMPs）和病原相关分子模式（PAMPs）识别及其在炎症反应中的调节作用。方法：提取并纯化野生型小鼠腹腔巨噬细胞, 用DAMPs中的高迁移率族蛋白Bl（HMGB1）和PAMPs中的脂多糖（LPS）分别刺激24 h，Real-time PCR方法检测细胞中Siglec-G基因的表达，ELISA方法检测野生型和Siglec-G基因敲除小鼠腹腔巨噬细胞培养上清中肿瘤坏死因子α （TNF-α）和白细胞介素6（IL-6）的分泌情况。结果：HMGB1能够使巨噬细胞中Siglec-G的表达明显升高，而LPS作用后Siglec-G的表达没有明显改变，同时发现，HMGB1作用于Siglec-G敲除小鼠的巨噬细胞后，TNF-α和IL-6的分泌明显增加，而LPS刺激后炎症因子的分泌没有明显改变。结论：巨噬细胞通过表达Siglec-G来识别DAMPs并发挥免疫抑制作用，而其对于PAMPs缺乏识别及调控作用。

Abstract:

Objective: To identify the PAMPs and DAMPs by Siglec-G, a member of the Sialic acid-binding immunoglobulin-like lectin (Siglecs) family, in macrophages and to explore its regulatory effect on PAMPs and DAMPs in inflammatory response. Methods: Peritoneal macrophages harvested and purified from wild type mice were stimulated with high mobility group protein Bl (HMGB1) in DAMPs and lipopolysaccharide (LPS) in PAMPs for 24 h, respectively. Real-time PCR was performed to analyze the Siglec-G mRNA expression in macrophages. ELISA was used to detect the secretion of TNF-α and IL-6 in the culture supernatants of peritoneal macrophages in wild type and Siglec-G knockout mice. Results: HMGB1 could significantly increase the expression of Siglec-G in macrophages, while the expression of Siglec-G was not altered after the stimulation of LPS. And it was found that the secretion of TNF-α and IL-6 increased significantly after HMGB1 acted on macrophages of Siglec-G knockout mice, but the secretion of inflammatory factors did not change markedly after LPS stimulation. Conclusion: Macrophages could recognize DAMPs by Siglec-G , and play a critical role in immune suppression, but it may not recognise or regulate PAMPs.

参考文献/References:

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相似文献/References:

备注/Memo

备注/Memo:

文章编号 １００６－８１４７（２０18）０2-0093-04
基金项目 国家自然科学基金资助项目（31370891，31240036）；天津市自然科学基金重点项目（13JCZDJC30000）
作者简介 樊亚童（1990-），女，硕士在读，研究方向：免疫学；通信作者：张学军，E-mail:xjzhimmunology@163.com。

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更新日期/Last Update: 2018-03-20