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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

22卷

期数:

2016年04期

页码:

347-351

栏目:

技术与方法

出版日期:

2016-07-19

文章信息/Info

Title:

Aqueous synthesis of CdTe quantum dots with fluorescence spectrophotometric for gentamicin sulfate detection

文章编号:

1006-8147（2016）04-0347-05

作者:

??庄布军¹; 黄 琛²; 张 莉²; 吴伯岳²; 高卫真¹; 2

(1.天津医科大学基础医学院药理学系，天津 300070；2.天津医科大学医学检验学院，天津 300203)

Author(s):

ZHUANG Bu-jun¹;  HUANG Chen²;  ZHANG Li²;  WU Bo-yue²;  GAO Wei-zhen¹; 2

(1.Department of Pharmacology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China; 2.School of Medical Laboratory, Tianjin Medical University, Tianjin 300203, China)

关键词:

line-height: 150%; " align="left">量子点; 水相合成; 硫酸庆大霉素; 荧光检测

Keywords:

quantum dots' target="_blank" rel="external">"> quantum dots;  aqueous synthesis;  gentamicin sulfate;  fluorescence detection

分类号:

R9

DOI:

-

文献标志码:

A

摘要:

目的：基于CdTe量子点的荧光淬灭现象，建立一种简便、经济和高灵敏度的检测硫酸庆大霉素的新方法。方法：以巯基乙酸（TGA）为稳定剂，在水相中合成CdTe量子点，并用荧光分光光度计、红外光谱仪、透射电镜对其进行表征。实验过程中，优化了CdTe量子点TGA、镉、碲三者之间的比例，考察了不同pH与回流时间对量子点荧光性质的影响。通过测定CdTe量子点荧光强度的下降，实现了对硫酸庆大霉素注射液浓度的测定。结果：合成了荧光发射峰在607 nm、粒径在4.0 nm左右的CdTe量子点。硫酸庆大霉素对CdTe 量子点的荧光有淬灭作用，在pH=7.4的磷酸盐缓冲体系中，硫酸庆大霉素的浓度在0.10～2.00 mg/L范围时，量子点荧光强度比值与硫酸庆大霉素浓度符合Stern -Volmer方程且相关系数R²=0.9989。结论：该方法灵敏度较高，快速简便，可用于硫酸庆大霉素浓度的测定。

Abstract:

Objective: To prepare a facile and cost efficient method for the determination of gentamicin sulfate . Methods: The thiogycolic acid capped CdTe quantum dots were synthesized in aqueous phase. Transmission electron microscopy, infrared spectroscopy and fluorescence spectroscopy were used to characterize the particle size and shape, the surface chemical group and the fluorescence property of the quantum dots. During the experiment, proportion of TGA, Cd, Te among CdTe quantum synthesis process were optimized, and the optical stability and the effect of pH on the fluorescence intensity of CdTe quantum dots were also evaluated. Results: The fluorescence emission peak at 607 nm and the TEM images displayed CdTe at about 4.0 nm were synthesized. The fluorescence quenching effect of gentamicin sulfate on CdTe quantum dots was researched, and a new method for the detection of gentamicin sulfate using CdTe quantum dots as fluorescent probes was established. In the optimal conditions, CdTe quantum dots were successfully applied to the detection of gentamicin sulfate in water, and a linear relationship was obtained to cover the concentration range of 0.10～2.00 mg/L, with a correlation coefficient of 0.9989, which was best described by a Stern–Volmer equation. Conclusion: The proposed method can be used for the determination of gentamicin sulfate with satisfactory results.

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金青年基金资助项目(21205087)；天津医科大学科学基金资助项目（2011KY11）

作者简介 庄布军(1991-)，男，硕士在读，研究方向：纳米材料在药物检测方面的应用；

通信作者：高卫真，E-mail：weizhengao33@163.com。

常用功能

更新日期/Last Update: 2016-07-13