|本期目录/Table of Contents|

[1]潘 杰,戴学文,李 晗,等. 4－氨基安替比林分光光度法测定苯酚滴耳液中苯酚含量[J].天津医科大学学报,2015,21(01):87-89.
　PAN Jie,DAI Xue-wen,LI Han,et al. Determination of phenol in phenol ear drops by 4–amino-antipyrine spectrophotometry[J].Journal of Tianjin Medical University,2015,21(01):87-89.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
21卷
期数:
2015年01期
页码:
87-89
栏目:
技术与方法
出版日期:
2015-01-20

文章信息/Info

Title:
 Determination of phenol in phenol ear drops by 4–amino-antipyrine spectrophotometry
文章编号:
1006-8147（2015）01-0087-03
作者:
 潘 杰1; 戴学文2; 李 晗2; 房志仲2
（1.天津市中心妇产科医院药剂科，天津 300100；2.天津医科大学药学院药剂学教研室，天津市临床药物关键技术重点实验室，天津 300070）
Author(s):
 PAN Jie1; DAI Xue-wen2; LI Han2; FANG Zhi-zhong2
 (1.Department of Pharmacy,Tianjin Center Hospital of Obstetrics and Gynecology,Tianjin 300100,China;2. Department of Pharmacy, College of Pharmacy, Tianjin Medical University, Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics)，Tianjin 300070,China)
关键词:
苯酚; 滴耳液; 4－氨基安替比林; 分光光度法; 含量测定
Keywords:
 ; phenol; ear drops; 4 - amino- antipyrine;  spectrophotometry;  content ;  determination

分类号:
R927.2
DOI:
-
文献标志码:
A
摘要:
目的：探讨一种更为简便、快速的测定苯酚滴耳液中苯酚含量的方法。方法：苯酚与4－氨基安替比林在铁氰化钾的作用下，溶液显橙红色，在520 nm处呈最大吸收，可直接用分光光度法测定苯酚的含量。结果：在520 nm测定波长下，苯酚浓度在1.0、1.5、2.0、2.5、3.0、3.5、4.0和4.5 μg/mL范围内呈良好的线性关系。回归方程：Y=0.1408X－4.4762×10-3(r=0.999 9,n=3)，测定方法的平均回收率99.72%，平均日内差和日间差RSD分别为0.44%和0.63%；3批样品的含量较为均匀，平均含量为0.196 g/mL，平均RSD为0.63%。结论：该方法较为简便，灵敏，结果准确可靠。
Abstract:
Objective: To develop a simple, effective method for content determination of phenol in phenol ear drops. Methods: Under the effect of potassium ferricyanide, the solution containing phenol and 4-aminoantipyrene showed orange red and the maximum absorption wavelength was 520 nm. The contents was directly determined by spectrophotometric method. Results: With the maximum absorption wavelength being 520 nm , the linear range of phenol was 1.0-4.5 μg/mL (r＝0.999 9,n=3),and the regression equation was Y=0.1408X－4.4762×10-3. The average recovery rate was 99.72%, and RSD of intra-day and inter-day precision were 0.44% and 0.63%, respectively. No significant difference was found in the contents of the samples determined by our method), with the average content being 0.196 g/mL and RSD 0.63%. Conclusion：The established method is simple, efficient and accurate.

参考文献/References:

[1] 中华人民共和国卫生部药政局.《中国医院制剂规范》[M].北京：中国医药科技出版社，1995:155-155

[2] 药典委员会.《中国药典》2010年版（二部）[M].北京：化学工业出版社，2010，325-326

[3]卢今，程钢，屈建，等. 炉甘石洗剂的质量控制[J]. 中国药师, 2013,16(7):1012

[4]朱红旗，薛子坤. 紫外分光光度法测定苯酚甘油滴耳液中苯酚的含量[J]. 齐鲁药事, 2006,25(6):344

[5]朱铁梁，杨波，郝素云. 紫外分光光度法测定苯酚滴耳液的含量[J]. 武警医学院学报, 2010,19(8):605

[6]陈晓峰，沈花. 高效液相色谱法测定消炎止痒醑中苯酚的含量[J]. 抗感染药学, 2011,8(4):268

[7] 国家环境保护局.水质挥发酚的测定-蒸馏后4-氨基安替比林分光度法[S].《中华人民共和国国家标准》，GB 7409-87，1987：130-138

相似文献/References:

[1]潘 杰,戴学文,李 晗,等.4－氨基安替比林分光光度法测定苯酚滴耳液中苯酚含量[J].天津医科大学学报,2015,21(03):87.
　PAN Jie,DAI Xue-wen,LI Han,et al.Determination of phenol in phenol ear drops by 4–amino-antipyrine spectrophotometry[J].Journal of Tianjin Medical University,2015,21(01):87.

备注/Memo

备注/Memo:
 作者简介 潘杰（1969-），男，主管药师，研究方向：临床药学；通信作者，房志仲，E-mail:fangzhizhong@tijmu.edu.cn.
更新日期/Last Update: 2015-06-11