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[1]李 青,郭 刚,Bilan Philip,等.电刺激促进大鼠骨骼肌细胞GLUT4转位并激活Akt信号通路[J].天津医科大学学报,2014,20(02):102-104.
　LI Qing,GUO Gang,Bilan Philip,et al.Electric pulse stimulation-induced GLUT4 translocation and activated Akt signaling pathway in L6-GLUT4myc-AS160 myotubes[J].Journal of Tianjin Medical University,2014,20(02):102-104.
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电刺激促进大鼠骨骼肌细胞GLUT4转位并激活Akt信号通路(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
20卷
期数:
2014年02期
页码:
102-104
栏目:
基础医学
出版日期:
2014-03-20

文章信息/Info

Title:
Electric pulse stimulation-induced GLUT4 translocation and activated Akt signaling pathway in L6-GLUT4myc-AS160 myotubes
文章编号:
1006-8147(2014)02-0102-03
作者:
李 青1; 郭 刚1; Bilan Philip2;  Klip Amira2
（1.天津医科大学代谢病医院内分泌研究所，卫生部激素与发育重点实验室，天津300070；2.加拿大病童医院细胞生物学实验室，多伦多 M5G1X8）
Author(s):
LI Qing1;  GUO Gang1;  Bilan Philip2;  Klip Amira 2
(1. Institute of Endocrinology, Metabolic Diseases Hospital, Tianjin Medical University, Key Laboratory of Hormone and Development, Ministry of Health, Tianjin 300070, China; 2. The Hospital for Sick Children, Program in Cell Biology, Toronto M5G 1X8, Canada)
关键词:
电刺激; 骨骼肌; 葡萄糖转运子4; 蛋白激酶B; AS160; 胰岛素
Keywords:
electrical pulse stimulation;  skeletal muscle;  glucose transporter 4;  PKB;  AS160;  insulin
分类号:
R58
DOI:
-
文献标志码:
A
摘要:
目的：利用L6细胞模型研究电脉冲刺激对骨骼肌细胞GLUT4转位及其信号通路的影响。方法：将稳定过表达GLUT4myc-AS160的L6-GLUT4myc-AS160大鼠骨骼肌细胞体外培养于6孔培养板中，用含2% 胎牛血清的细胞分化液诱导分化为肌管，将6孔板随机分成对照组、胰岛素组和电脉冲刺激（EPS）组，用终浓度为100 nmol/L的胰岛素或电刺激仪对细胞刺激，对照组不做任何处理。ELISA测定细胞膜上的GLUT4myc含量，免疫印记法测定蛋白激酶B(Akt) 和Rab-GTPase 激活蛋白AS160的磷酸化。结果：与对照组相比，1h电刺激显著增加细胞膜表面GLUT4myc水平并磷酸化Akt及Rab-GTPase 激活蛋白AS160。结论：电刺激促进L6骨骼肌细胞GLUT4myc转位，激活Akt信号通路。
Abstract:
Objective: To study the electric pulse stimulation (EPS)-stimulated signal transduction and GLUT4myc translocation in L6-GLUT4myc-AS160 myotubes. Methods: L6-GLUT4myc-AS160 myoblasts were incubated in 6-well plates, differentiated to myotubes with 2% FBS differentiation media, after which they were divided into 3 groups (EPS, insulin, control) with the first two treated with 100 nmol/L insulin or contract cell and the control group receiving no treatment. The amount of GLUT4myc on the cell surface was measured by ELISA and the phosphorylation of protein kinase B(Akt)and Rab-GTPase AS160 were obtained by immunoblotting. Results: Compared to the control group, EPS stimulated GLUT4myc translocation significantly in 1h and the Akt and AS160 were both phosohorylted by EPS. Conclusion: EPS-induced GLUT4myc can be translocated to the cell surface and activate the Akt signaling pathway.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介 李青（1987—），女，硕士在读，研究方向：生物化学与分子生物学；通信作者：郭刚 ，E-mail: guogangtj@126.com。
更新日期/Last Update: 2014-03-30