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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

19卷

期数:

2013年06期

页码:

441-444

栏目:

基础医学

出版日期:

2013-11-20

文章信息/Info

Title:

Effects of fenofibrate on insulin resistance in severely scalded mice

作者:

张 建¹; 张明谏²;  刘 曼¹; 陈宗南¹; 王玉亮³; 李小兵²; 刘子健²; 李 钢²

1.天津医科大学一中心临床学院整形与烧伤科，天津300192；2.天津市第一中心医院整形与烧伤科，天津300192；3.卫生部危重病急救医学重点实验室，天津300384

Author(s):

ZHANG Jian¹;  ZHANG Ming-jian²;  LIU Man¹;  CHEN Zong-nan¹;  WANG Yu-liang³;  LI XIAO-bing²;  LIU ZI -jian²;  LI Gang²

１．Ｄepartment of Plastic and Burn, The First Central Clinical College ,Tianjin Medical University, Tianjin 300192, China; 2.Department of Plastic and Burn, The First Center Hospital of Tianjin，Tianjin 300192, China; 3.The Key Laboratory of Critical Care of Medi cine Ministry of Health, Tianjin 300384, Chｉｎａ

关键词:

非诺贝特; 烫伤; 小鼠; 应激反应; 胰岛素抵抗

Keywords:

-

分类号:

R644

DOI:

-

文献标志码:

A

摘要:

目的：观察非诺贝特对严重烫伤小鼠应激状态下胰岛素抵抗的作用。方法：130只SPF级昆明小鼠，随机分为：低剂量组（Ⅰ）、高剂量组（Ⅱ）和对照组（Ⅲ）各40只,正常组10只。将小鼠制成Ⅲ°30%全身体表面积（TBSA）烫伤模型，正常组不烫伤，烫伤后Ⅰ、II组分别予以30 mg·kg-1·d-1、100 mg·kg-1·d-1非诺贝特灌胃，ＩＩI组予以0.5 mL·d-1生理盐水灌胃。各组分别于第1、3、7、14天随机抽取10只小鼠（正常组一次性处死），处死收集血清，测定空腹血糖、血清胰岛素、TNF-α、游离脂肪酸（ＦＦＡ）水平，运用稳态模式法（HOMA）计算胰岛素抵抗（IR）指数，同时切取肝脏组织光镜下观察其病理变化。 结果：烫伤后各组小鼠在第1、3、7天HOMA-IR值均高于正常组，I组和II组HOMA-IR值均低于III组。Ⅰ、Ⅱ、Ⅲ各组小鼠血清TNF-α、FFA水平在伤后不同时间点均高于正常组，I组和II组均低于III组（P<0.05或P<0.01），但I组和II组在同一时间点均值比较差异无统计学意义（P＞0.05）。各组不同时间点比较差异均有统计学意义（P＜0.05）。烫伤后小鼠肝脏有不同程度的脂肪变性，给予诺贝特干预后，肝脏脂肪变性有所改善。结论：非诺贝特可以改善烧（烫）伤后小鼠应激状态下IR，其可能与降低严重烫伤小鼠血清TNF-α和FFA水平，减轻肝脏脂肪变性有关。

Abstract:

Objective: To observe the effect of fenofibrate on insulin resistance in severely scalded mice. Methods: 130 SPF KM mice were divided into 4 groups randomly: low dose group ( I ) (40), high dose group (II) (40), control group (III) (40) and normal group (10). Healthy mice were subjected to a 30% TBSA third-degree scald injury to develop a mice model of scald injury (The normal group was not given any burn). Mice in group I and II were taken fenofibrate 30 mg·kg-1·d-1, 100 mg·kg-1·d-1 respectively. Mice in the III group were taken physiological saline 0.5 mL·d-1 .10 mice in each group were killed in fasting condition on days 1, 3, 7, 14 d (the normal group were all killed in one time), after collecting the blood samples, testing FBG , serum insulin (FINS), TNF-alpha level, and serum free fatty acid (FFA) levels, calculating IR value by method of the steady-state model (HOMA). the liver tissue and observe the pathological changes of the liver tissue by the light microscope were also collected. Results: In each group, the number of mice after burn at days 1, 3 and 7, HOMA-IR values was higher than that of normal group. Compared with group III, group I and II had significantly lower number. The serum TNF-alpha and the FFA levels in mice of each group after burn was higher than that of the normal group. Compared with group III, group I and II were significantly lower in number at the same point (P＜0.01 or P<0.05), but the difference between group I and group II was not statistically significant (P>0.05). The differences were statistically significant (P<0.05) in each group at different time noints. Fenofibrate could improve the different level of fatty degeneration of mice liver after severe scald. Conclusion: Fenofibrate can

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备注/Memo

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更新日期/Last Update: 2013-12-20