«上一篇/Previous Article|本期目录/Table of Contents|下一篇/Next Article»

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

21卷

期数:

2015年03期

页码:

29-34

栏目:

基础医学

出版日期:

2015-05-20

文章信息/Info

Title:

Magnetic mesoporous silica nanoparticles for drug delivery and photodynamic therapy

文章编号:

1006-8147（2015）0-0029-06

作者:

汪文媚¹; 吴伯岳²; 要 旸²; 高卫真¹; 2

(1.天津医科大学基础医学院药理学教研室,天津 300070;2.天津医科大学医学检验学院,天津 300203)

Author(s):

WANG Wen-mei¹;  WU Bo-yue²;  YAO Yang²;  GAO Wei-zhen¹; 2

(1.Department of Pharmacology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China; 2. School of Medical Laboratory, Tianjin Medical University, Tianjin 300203, China)

关键词:

介孔二氧化硅; 磁靶向性; 刺激响应; 药物传输; 光动力治疗

Keywords:

mesoporous silica nanoparticles;  magnetic targeting ;  stimuli response;  drug delivery;  photodynamic therapy

分类号:

R9

DOI:

-

文献标志码:

A

摘要:

目的：制备温度和pH双重响应的核壳结构磁性荧光介孔二氧化硅纳米粒子用于抗肿瘤药物传输以及协同光动力治疗。方法：采用溶剂热法、反相胶束法制备实心硅包覆的Fe₃O₄核，以改良的溶胶凝胶法制备介孔硅中间层，再以种子沉淀聚合法在介孔硅表面修饰温敏聚合物壳层，得到Fe₃O₄@SiO₂(F)@mSiO₂(P)@P(NIPAM-co-AA)纳米粒子。利用透射电子显微镜(TEM)对其形貌进行了表征。以盐酸阿霉素为模型药考察了该纳米粒子对药物的负载与释放行为，并采用MTT比色法对其进行了体外细胞活性评价。结果：TEM表征结果显示，该纳米粒子平均粒径约为300 nm。药物负载与释放结果表明，该纳米粒子不仅具有较高的载药量(206.75±17.59) μg/mg和包封率(68.91±5.86) wt%，药物释放也呈现明显的温度和pH依赖性。MTT结果表明，载药的纳米粒子在680 nm LED 灯照射条件下与单用化学治疗和光动力治疗相比，对细胞的毒性明显增大(P<0.01)。结论：Fe₃O₄@SiO₂(F)@mSiO₂(P)@P(NIPAM-co-AA)纳米粒子可作为一个抗肿瘤药物载体，实现肿瘤化疗和光动力治疗的协同研究。

Abstract:

Objective: To prepare temperature-pH responsive and core-shell-structured magnetic fluorescent mesoporous silica nanoparticles for antitumor drug delivery and photodynamic therapy. Methods: First, the core of nonporous silica coated Fe₃O₄ was prepared via solvothermal reaction and reverse micelle method, then mesoporous silica as the middle layer was further coated on the core by modified sol-gel process, and finally the Fe₃O₄@SiO₂(F)@mSiO₂(P)@P(NIPAM-co-AA) nanoparticles were prepared with the polymer shell modified on the middle layer through seed precipitation polymerization. Transmission electron microscopy (TEM) was carried out to characterize the morphology of the nanoparticles. Doxorubicin hydrochloride (DOX) was used as a model drug to investigate the drug loading and releasing behavior. And MTT assay was adopted to evaluate the biocompatibility and cytotoxicity of the nanoparticles. Results: The results of TEM showed that the average diameter of the nanoparticles was 300 nm. The drug loading and releasing results indicated that the nanoparticles exhibited an excellent drug loading content of (206.75±17.59) μg/mg and encapsulation efficiency of (68.91±5.86) wt%, and controlled drug releasing could be obtained by changing the temperature or pH values. MTT assay showed that the cytotoxic effect of DOX-loaded nanoparticles irradiated with a 680 nm LED lamp was significantly higher than that achieved by chemotherapy and photodynamic therapy alone. Conclusion: Fe₃O₄@SiO₂(F)@mSiO₂(P)@P(NIPAM-co-AA) nanoparticles could be used as an antitumor drug carrier to achieve a synergistic effect by combining chemotherapy and photodynamic therapy.

参考文献/References:

[1] Kresge C T, Leonowicz M E, Roth W J, et al. Ordered mesoporous molecular sieves synthesized by a liquid-crystal template mechanism[J]. Nature, 1992, 359(6397): 710
[2] Slowing I I, Vivero-Escoto J L, Wu C W, et al. Mesoporous silica nanoparticles as controlled release drug delivery and gene transfection carriers[J]. Adv Drug Deliv Rev, 2008, 60(11): 1278
[3] Chen Y Y, Ma P A, Yang D M, et al. Multifunctional core-shell structured nanocarriers for synchronous tumor diagnosis and treatment in vivo[J]. Chem Asian J, 2014, 9(2): 506
[4] Liu J L, Li C Y, Li F Y, et al. Fluorescence turn-on chemodosimeter-functionalized mesoporous silica nanoparticles and their application in cell imaging[J]. J Mater Chem, 2011, 21(20): 7175
[5] Kang X J, Cheng Z Y, Yang D M, et al. Design and synthesis of multifunctional drug carriers based on luminescent rattle-type mesoporous silica microspheres with a thermosensitive hydrogel as a controlled switch[J]. Adv Funct Mater, 2012, 22(7): 1470
[6] Teng I T, Chang Y J, Wang L S, et al. Phospholipid-functionalized mesoporous silica nanocarriers for selective photodynamic therapy of cancer[J]. Biomaterials, 2013, 34(30): 7462
[7] Wu S H, Hung Y, Mou C Y, et al. Mesoporous silica nanoparticles as nanocarriers[J]. Chem Commun, 2011, 47(36): 9972
[8] Celli J P, Spring B Q, Rizvi I, et al. Imaging and photodynamic therapy: mechanisms, monitoring, and optimization[J]. Chem Rev, 2010, 110(5): 2795
[9] Verma S, Watt G M, Mai Z M, et al. Strategies for enhanced photodynamic therapy effects[J]. Photochem & Photobio, 2007, 83(5): 996
[10] Tu J, Wang T X, Shi W, et al. Multifunctional ZnPc-loaded mesoporous silica nanoparticles for enhancement of photodynamic therapy efficacy by endolysosomal escape[J]. Biomaterials, 2012, 33(31): 7903
[11] Hilger I, Kaiser W A. Iron oxide-based nanostructures for MRI and magnetic hyperthermia[J]. Nanomedicine, 2012, 7(9): 1443
[12] Vallet-Regi M, Ramila A, del Real R P, et al. A new property of MCM-41: Drug delivery system[J]. Chem Mater, 2001, 13(2): 308
[13] Gerweck L E, Seetharaman K. Cellular pH gradient in tumor versus normal tissue: potential exploitation for the treatment of cancer[J]. Cancer Res, 1996, 56(6): 1194
[14] 刘聪颖, 胡建华, 杨东,等. 多重响应性介孔二氧化硅纳米微球的制备及载药研究[J]. 化学学报 , 2009, 67(8): 843
[15] Wu H X, Tang L H, An L, et al. pH-responsive magnetic mesoporous silica nanospheres for magnetic resonance imaging and drug delivery[J]. React Funct Polym, 2012, 72(5): 329

相似文献/References:

[1]汪文媚,吴伯岳,要 旸,等.磁性介孔二氧化硅用于药物传输和光动力治疗[J].天津医科大学学报,2015,21(01):29.
　WANG Wen-mei,WU Bo-yue,YAO Yang,et al.Magnetic mesoporous silica nanoparticles for drug delivery and photodynamic therapy[J].Journal of Tianjin Medical University,2015,21(03):29.

备注/Memo

备注/Memo:

基金项目 国家自然科学基金青年基金资助项目(21205087)

作者简介 汪文媚(1989-)，女，硕士在读，研究方向：纳米材料在药物传输方面的应用；

通信作者：高卫真，E-mail：weizhengao33@163.com。

常用功能

更新日期/Last Update: 2015-01-22